The largest database of trusted experimental protocols

Antibody anti cd147

Manufactured by Santa Cruz Biotechnology
Sourced in United States

The Antibody anti-CD147 is a laboratory research tool used for the detection and study of the CD147 protein. CD147, also known as Basigin or EMMPRIN, is a cell surface glycoprotein that plays a role in various cellular processes. This antibody can be used in techniques such as Western blotting, immunohistochemistry, and flow cytometry to identify and quantify the expression of CD147 in different cell types and tissues.

Automatically generated - may contain errors

3 protocols using antibody anti cd147

1

Antibody-based SARS-CoV-2 Spike Protein Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibody anti-CD147 was from Santa Cruz Biotechnology (Dallas, TX, USA). Antibody anti-Spike protein antibody was from Genetex (Alton Pkwy Irvine, CA, USA). Antibodies anti-ACE2, anti-GAPDH, and anti-CyPA were from Abcam (Cambridge, Cambridgeshire, UK). HRP-conjugated secondary antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA) unless otherwise stated. Hoechst 33542, Alexa 488-, and Alexa 546-conjugated secondary antibodies were obtained from Molecular Probes (Life Technologies by Thermo Scientific, Waltham, MA, USA). CsA, DMSO, HEPES, MTT, Tris, Glycine, SDS, Tween 20, saponin, NH4Cl, and bovine serum albumin (BSA) were purchased from Sigma (by Merck, Kenilworth, NJ, USA). All cell culture reagents were from Thermo Scientific. All chemical reagents were of analytical grade or higher, and purchased from Sigma unless otherwise specified.
+ Open protocol
+ Expand
2

Antibody-based Protein Detection Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibody anti-CD147 was from Santa Cruz Biotechnology (Dallas, TX, USA). Antibody anti-Spike protein antibody was from Genetex (Alton Pkwy, Irvine, CA, USA). Antibodies anti-ACE2, anti-GAPDH and anti-CyPA were from Abcam (Cambridge, UK). Blocking anti-CD147 (MEM-M6/6) antibody was from Bio-Rad Laboratories (Hercules, CA, USA). Mouse IgG were purchased from Jackson ImmunoResearch Laboratories (West Grove, PA, USA). HRP-conjugated secondary antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Hepes, Tris, Glycine, SDS, tween 20, saponin, NH4Cl, and bovine serum albumin (BSA) were purchased from Sigma (by Merck, Kenilworth, NJ, USA). All cell culture reagents were from Thermo Scientific (Waltham, MA, USA). All chemical reagents were of analytical grade or higher, and purchased from Sigma unless otherwise specified.
+ Open protocol
+ Expand
3

Antibody-based SARS-CoV-2 Spike Protein Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibody anti-CD147 was from Santa Cruz Biotechnology (Dallas, TX, USA). Antibody anti-Spike protein antibody was from Genetex (Alton Pkwy Irvine, CA, USA). Antibodies anti-ACE2, anti-GAPDH, and anti-CyPA were from Abcam (Cambridge, Cambridgeshire, UK). HRP-conjugated secondary antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA) unless otherwise stated. Hoechst 33542, Alexa 488-, and Alexa 546-conjugated secondary antibodies were obtained from Molecular Probes (Life Technologies by Thermo Scientific, Waltham, MA, USA). CsA, DMSO, HEPES, MTT, Tris, Glycine, SDS, Tween 20, saponin, NH4Cl, and bovine serum albumin (BSA) were purchased from Sigma (by Merck, Kenilworth, NJ, USA). All cell culture reagents were from Thermo Scientific. All chemical reagents were of analytical grade or higher, and purchased from Sigma unless otherwise specified.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!