C myc tag

Manufactured by Santa Cruz Biotechnology

Sourced in United States, Canada

The C-myc tag is a short amino acid sequence that can be added to a protein of interest to facilitate its detection and purification. It is a widely used tool in molecular biology and biochemistry research.

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C-Myc (386 citations) Myc-tag (22 citations)

3 protocols using c myc tag

Investigating EA.hy926 Endothelial Cells

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EA.hy926 cells (American Type Culture Collection, ATCC) were grown in 60-mm Petri dishes in Dulbecco’s Modified Eagle Medium (DMEM, with 4.5 g/L glucose, L-glutamine, and sodium pyruvate) with 10% FBS. Cells were cultured at 37 °C in 5% CO₂ and passaged with trypsin-EDTA. Then, the cell culture medium was changed to DMEM with 1% fatty acid-free Bovine Serum Albumin (BSA) and cultured for 24 h before the experiment. ApoM antibody was purchased from GeneTex (CA, USA), and FVIII, S1PR2, S1PR4, S1PR5, and C-myc tag antibodies were obtained from Santa Cruz (CA, USA). S1PR1, S1PR3, and His tag antibodies and FITC-conjugated secondary antibodies were purchased from Abcam (Cambridge, UK). Cy3-conjugated secondary antibody was purchased from Bethyl (TX, USA). Deep-sea fish gelatin was obtained from Sigma (CA, USA). 4'6-diamidino-2-phenylindole (DAPI) dye and anti-fade solution were sourced from Boster Biological Engineering Company (Wuhan, China). Cell culture plates (6-well and 24-well) were purchased from Costar (NY, USA).

Shi Y., Liang Y., Zhang J., Yu M., Wang M., Zheng L., Di D., Zhang X., Luo G, & Xu N. (2020). Non-negligible factors in studying the ApoM-S1P axis using EA.hy926 cells. Annals of Translational Medicine, 8(6), 383.

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Antibody Catalog for Cellular Signaling

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Rabbit antibodies against TLR7, MyD88, NF-κB p65, and phosphorylated IκBα (p-IκBα, Ser32) were purchased from Abcam (Cambridge, United Kingdom). Mouse antibodies against HRS, IRAK1, and c-Myc Tag, goat antibodies to TAB1, and normal rabbit IgG were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Rabbit antibodies against phosphorylated NF-κB p65 (p-p65, Ser536), phosphorylated p38 MAPK (p-p38, Thr180/Tyr182), p38 MAPK, Clathrin, Calnexin, EEA1, Rab5, Rab7, Rab11, LAMP1, Rcas1, and TRAF6 were purchased from Cell Signaling Technology (Beverly, MA). Rabbit antibodies against GFP, PKCζ, β-actin, and GAPDH were purchased from ProteinTech Group (Chicago, IL). Mouse antibody to EV71 VP1 was from Abnova Company (Taipei). Rabbit antibody to EV71 3C was raised against residues 76–88 of 3C protein (Abgent, Suzhou, China). Rat antibodies to FLAG, and anti-mouse CD68 were purchased from BioLegend (San Diego, CA).

Luo Z., Ge M., Chen J., Geng Q., Tian M., Qiao Z., Bai L., Zhang Q., Zhu C., Xiong Y., Wu K., Liu F., Liu Y, & Wu J. (2017). HRS plays an important role for TLR7 signaling to orchestrate inflammation and innate immunity upon EV71 infection. PLoS Pathogens, 13(8), e1006585.

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Immunofluorescence Assay for Bordetella

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B. bronchiseptica harboring pIVET-IP vector or pIVET-PBB0324 was grown to mid-log phase, pelleted by centrifugation and resuspended in D-PBS(À). The resuspended cells were immobilized on a cover glass that had been previously treated with 0.1% poly-L-lysine. Unbound bacteria were removed by three washes with D-PBS(À), after which the remaining bacteria were probed with mouse monoclonal antibodies against c-Myc tag (Santa Cruz) and FITC-conjugated goat antimouse antibody (Invitrogen). Between each step, the cover glass was washed three times with D-PBS(À) containing 0.1% BSA. The bacteria were observed under a fluorescence microscope (BX51; Olympus, Tokyo, Japan).

Abe H., Kamitani S., Fukui-Miyazaki A., Shinzawa N., Nakamura K, & Horiguchi Y. (2015). Detection of genes expressed in Bordetella bronchiseptica colonizing rat trachea by in vivo expressed-tag immunoprecipitation method. Microbiology and immunology, 59(5).

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