The chemicals and solvents used in this study were purchased from various chemical vendors: Sigma-Aldrich (Pty) Ltd. (Johannesburg, South Africa), Merck (Pty) Ltd. (Johannesburg, South Africa) and were used without purification. The progress of the reactions was monitored by thin-layer chromatography (TLC) using Merck 60F254 silica gel plates (Merck, Johannesburg, South Africa) supported on aluminium, and the plates were visualized under ultraviolet (UV254 and 366 nm) light or stained with iodine vapour. 1H and 13C NMR spectra were recorded on Bruker Biospin 600 MHz spectrometer, and the chemical shifts are given in values referenced to deuterated DMSO-d6 and are reported in parts per million (ppm). Chemical shifts for deuterated DMSO-d6 appear at 2.5 ppm for 1H and 39.5 ppm for 13C NMR spectra. Proton coupling patterns are abbreviated as follows: s (singlet), d (doublet), dd (doublet of doublet), t (triplet), q (quartet), and m (multiplet). Coupling constants (J) are reported in Hz. NMR data were analysed using MestReNova Software, version 5.3.2–4936. Melting points (mp) were established with a Büchi melting point B-545 instrument and were uncorrected. The High-resolution mass spectra (HRMS) were recorded using a Bruker micrOTOF-Q II mass spectrometer using atmospheric pressure chemical ionization (APCI) in positive ion mode.
Biospin 600 mhz spectrometer
Manufactured by Bruker
The Bruker Biospin 600 MHz spectrometer is a high-resolution nuclear magnetic resonance (NMR) instrument designed for advanced analytical applications. It operates at a magnetic field strength of 600 MHz, providing high-quality data for the study of molecular structures and dynamics. The Biospin 600 MHz spectrometer is a core analytical tool for researchers and scientists across various fields, including chemistry, biochemistry, and material science.
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2 protocols using biospin 600 mhz spectrometer
1
Analytical Characterization of Organic Compounds
2
Analytical Techniques for Biomolecular Characterization
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Liquid chromatography high-resolution mass spectrometry (LC-HRMS) analysis was performed on an Agilent Technologies 6520 Accurate-Mass Q-TOF LC-MS instrument with an Agilent Eclipse Plus C18 column (4.6 × 100 mm). A linear gradient of 2–95% CH3CN (vol/vol) over 20 min in H2O with 0.1% formic acid (vol/vol) at a flow rate of 0.2 mL/min was used. High-performance liquid chromatography (HPLC) analysis was carried out with Nexera XR HPLC apparatus using an Agilent 5 TC-C18 (2) (250 × 4.6 mm). Nuclear magnetic resonance (NMR) spectra were recorded on a Bruker Biospin 600 MHz spectrometer. Polymerase chain reaction (PCR) primers were synthesized by Tsingke Biological Technology. Kits for plasmid preparation and DNA extraction were from Omega. Standard molecular biology methods were used for all other DNA manipulations. NG-hydroxyl-L -arginine was purchased from Sigma. 8-AZG was purchased from Aladdin.
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