Flagellin from salmonella typhimurium tlrl stfla

Recombinant murine IL-1β (#211-11B), IL-6 (#216–16), IL-10 (#210–10), and IL-22 (#210–22) were purchased from PeproTech (Cranbury, NJ, USA). Recombinant mouse IL-23 (#1877-ML) and anti-SAA1 goat antibody (#AF2948) were purchased from R&D Systems (Minneapolis, MN, USA). Flagellin from Salmonella typhimurium (#tlrl-stfla) was purchased from InvivoGen (San Diego, CA, USA). LPS was purchased from Sigma-Aldrich (St. Louis, MO, USA). TNF-α (#203–14261) was purchased from Fujifilm Wako Pure Chemical Corporation (Osaka, Japan). Anti-p-NF-κB rabbit antibody (#3033) and inhibitor kappa Bα (IκBα) antibody (#9242) were purchased from Cell Signaling Technology (Danvers, MA, USA). 5-aminosalicylic acid (5-ASA) was provided by Kyorin Pharmaceutical Co. (Tokyo, Japan), and was dissolved in culture medium at 40 mmol/L (pH 7.2 adjusted with NaOH) as described previously [14 (link)]. The NF-κB inhibitor BAY11-7082 (#T2846) was purchased from Tokyo Kasei (Tokyo, Japan).