Inveon micro ct spect pet system

Micro computed tomography (microCT) was performed with a multi-modal Siemens Inveon micro-CT/SPECT/PET system (Inveon, Siemens Inc., Knoxville, TN, USA) to image implants within an intact head preparation following perfusion with PBS and 4% PFA, but before explantation for histology. The following parameters were used: 360 projection, 2×2 binning, 31 μm pixel size/isotropic resolution, 45.23 mm transaxial field of view (FOV), 2880 transaxial pixels, 32.16 mm axial FOV, 2048 axial pixels, 80 KV voltage, 500 μA current, and 560 ms exposure time.

Micro computed tomography (microCT) of explanted muscles with implanted SW or SS wire was obtained with a multi-modal Siemens Inveon micro-CT/SPECT/PET system (Inveon, Siemens Inc., Knoxville, TN, USA). The following parameters were used: 360° projections, 2 × 2 binning, 39 μm pixel size/ isotropic resolution, 80 KV voltage, 500 μA current, and 560 ms exposure time. Magnetic resonance (MR) images of explanted muscles with implanted SW or SS were acquired using a 7-tesla human MRI system (MEG-NETOM, Siemens). The MR pulse sequence used was Turbo spin echo imaging (TSE), with an echo time (TE) of 74 ms, and a repetition time (TR) of 11,210 ms. MRI images were visualized using Horos DICOM (https://horosproject.org).

To compare and contrast these murine findings with higher order animals, we examined the ex vivo meningeal collagen around a 10×10 shank Utah style array (Blackrock Microsystems, Salt Lake City, UT) that had been implanted in the motor cortex of a rhesus macaque for ~2.5 years (tissue was donated by the Motorlab, directed by Dr. Andrew Schwartz, at University of Pittsburgh). Following transcardial perfusion with 4% paraformaldehyde, the brain was removed with the Utah array and surrounding bone left intact. After post-fixation in 10% paraformaldehyde, micro computed tomography (microCT) was performed with a multi-modal Siemens Inveon micro-CT/SPECT/PET system (Inveon, Siemens Inc., Knoxville, TN, USA) as previously described[37 (link)]. The Utah array and surrounding tissue (>5mm margin) was dissected out of the brain. Brain tissue adjacent to one side of the Utah array was sectioned away with a Vibratome until the shanks of one edge of the device were visible. These shanks were imaged for second harmonic generation imaging as described in section 2.3.