Laminin α4

Manufactured by Merck Group

Laminin-α4 is a glycoprotein component of the extracellular matrix. It is a structural element that provides support and attachment for cells within tissues.

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Lab products found in correlation

α actinin, by Merck Group (2 mentions) Laminin α2, by Merck Group (2 mentions) Laminin α1, by R&D Systems (2 mentions) Laminin β1γ1, by Merck Group (2 mentions) Gapdh, by Cell Signaling Technology (2 mentions) Laminin α5, by Merck Group (1 mentions) F4 80, by Abcam (1 mentions)

Close spelling variants of this product

Laminin (1233 citations)

2 protocols using laminin α4

Immunostaining and Western Blot Antibodies

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For immunostaining and western blot analysis, the following antibodies were used: α-actinin (Sigma, catalog no. A7732; 1:5,000), agrin [for detection of mag; produced in-house (44 (link)); 1:5,000 for western blots and 1:200 for immunostainings or R&D System catalog no. AF550; 5 µg/mL for immunostainings], laminin-α1 (for detection of αLNNd by western blot; R&D Systems, catalog no. AF4187; 1:2,000), αLNNd [for detection of αLNNd by immunostainings, previously described (21 (link)); 1:100], laminin-α2 (Sigma, catalog no. L0663, clone 4H8-2; 1:500), laminin-α4 [previously described (21 (link)); 1:1,000 for western blots and 1:200 for immunostainings], laminin-β1γ1 (Sigma, catalog no. L9393; 1:100), and GAPDH (Cell Signaling, catalog no. 2118, 1:1,000).

Reinhard J.R., Porrello E., Lin S., Pelczar P., Previtali S.C, & Rüegg M.A. (2023). Nerve pathology is prevented by linker proteins in mouse models for LAMA2-related muscular dystrophy. PNAS Nexus, 2(4), pgad083.

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Immunostaining and Western Blot Antibody Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

For immunostaining and Western blot analysis, the following antibodies were used: α-actinin (1:5000; Sigma, catalog no. A7732), agrin [chicken, for detection of mag, produced in-house (56 (link)), 1:1000 for Western blots, 1:200 for immunostainings], eMHC (1:100 for Western blot, 1:1200 for immunostainings; developed by H. Blau, obtained from Developmental Studies Hybridoma Bank, catalog no. F1.652), F4/80 (1:100; Abcam, catalog no. ab6640), laminin-α1 (for detection of αLNNd by Western blot, 1:2000; R&D Systems, catalog no. AF4187), αLNNd [for detection of αLNNd by immunostainings, 1:100 (21 (link))], laminin-α2 [for Western blot analysis (57 (link)), 1:500], laminin-α2 (N-terminal, for immunostainings of human samples, clone 4H8-2, 1:400; Sigma, catalog no. L0663), laminin-α2 (C-terminal, for immunostainings of human samples, clone 5H2, 1:5000; Merck Millipore, catalog no. MAB1922), laminin-α4 (21 (link)) (1:1000 for Western blots, 1:200 for immunostainings), laminin-α5 [clone 504, gift from L. Sorokin (58 (link)), 1:1000], laminin-β1γ1 (1:1000 for Western blots, 1:100 for immunostainings; Sigma, catalog no. L9393), laminin-γ1 (1:200; Millipore, catalog no. MAB1914), Na⁺/K⁺-ATPase (1:1000; Cell Signaling, catalog no. 3010), GAPDH (1:1000; Cell Signaling, catalog no. 2118), and CD31 (1:100; Abcam, catalog no. ab9498).

Reinhard J.R., Lin S., McKee K.K., Meinen S., Crosson S.C., Sury M., Hobbs S., Maier G., Yurchenco P.D, & Rüegg M.A. (2017). Linker proteins restore basement membrane and correct LAMA2-related muscular dystrophy in mice. Science translational medicine, 9(396), eaal4649.

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