2 4 6 tripiridyl s triazine

Essential and non-essential amino-acid standards came from Sigma–Aldrich Co. (St. Louis, MO, USA). Sigma–Aldrich provided the Folin–Ciocalteu reagent, 2,2-diphenyl-1-picrylhydrazyl (DPPH),2,4,6-tripiridyl-s-triazine (TPTZ), 2,2′-azino-bis (3-thylbenzthiazoline- 6-sulphonic acid) (ABTS), α-amylase (from Aspergillus oryzae), α -Glucosidase (from Bacillus stearothermophilus), p-nitrophenyl—d-glucopyranoside (PNP-G), bovine serum al-bumin (BSA), sodium azide, and protease (from Bacillus licheniformis). Reagents for the HPLC came from Merck, Darmstadt, Germany.

All standards were obtained from Sigma–Aldrich Co. (St. Louis, MO, USA), including the essential amino acids: phenylalanine, valine, tryptophan, threonine, isoleucine, methionine, histidine, arginine, lysine and leucine. The non-essential amino acids studied were glycine, glutamic acid, aspartic acid, glutamine, serine, tyrosine, alanine and asparagine. The antioxidant activity determination reagents were obtained from Sigma–Aldrich Co: Folin–Ciocalteu reagent, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,4,6-tripiridyl-s-triazine (TPTZ), 2,2′-azino-bis (3-thylbenzthiazoline- 6-sulphonic acid) (ABTS). The solvents and reagents used in the HPLC analysis were purchased from Merck (Darmstadt, Germany). The α-amylase (from Aspergillus oryzae), α-Glucosidase (from Bacillus stearothermophilus), p-nitrophenyl-α-D-glucopyranoside (PNP-G), bovine serum albumin (BSA) and sodium azide were purchased from Sigma–Aldrich Co.