The high purity (≥97%) standards, including ascorbic acid, vanillic acid, syringic acid, epicatechin, daidzin, glycitin, ferulic acid, genistin, daidzein, glycitein, quercetin, and genistein were purchased from Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Other reagents of analytical grade. Soybeans (Liaodou, yellow seed coat, mainly planted in northern China, weigh 21.6 g of one hundred seeds) were obtained from a local market. R. oligosporus RT-3 isolated, screened and identified from Tempeh in our laboratory was used for soybean fermentation (11 (link), 12 ). The strain was cultivated on potato dextrose agar at 30°C for 3 days. Then, spores produced were scraped in water (1 × 107 spores/mL).
Genistin
Manufactured by Yuanye Bio-Technology
Sourced in China
Genistin is a laboratory reagent used in biochemical and molecular biology research. It is a plant-derived isoflavone compound that can be used as a standard or reference material in various analytical techniques. Genistin serves as a core component in specific assays and experiments, but its detailed applications should be determined by the research objectives and experimental protocols.
Automatically generated - may contain errors
Lab products found in correlation
Daidzin, by Yuanye Bio-Technology (10 mentions)
Daidzein, by Yuanye Bio-Technology (8 mentions)
Genistein, by Yuanye Bio-Technology (8 mentions)
Glycitin, by Yuanye Bio-Technology (6 mentions)
Glycitein, by Yuanye Bio-Technology (5 mentions)
Ferulic acid, by Yuanye Bio-Technology (3 mentions)
Acetonitrile, by Thermo Fisher Scientific (3 mentions)
Ononin, by Yuanye Bio-Technology (3 mentions)
Epicatechin, by Yuanye Bio-Technology (2 mentions)
Quercetin, by Yuanye Bio-Technology (2 mentions)
Rutin, by Yuanye Bio-Technology (2 mentions)
P coumaric acid, by Yuanye Bio-Technology (2 mentions)
Formic acid, by Thermo Fisher Scientific (2 mentions)
Biochanin a, by Yuanye Bio-Technology (2 mentions)
Formononetin, by Yuanye Bio-Technology (2 mentions)
Calycosin 7 o β d glucoside, by Yuanye Bio-Technology (2 mentions)
Syringic acid, by Yuanye Bio-Technology (1 mentions)
Ascorbic acid, by Yuanye Bio-Technology (1 mentions)
Vanillic acid, by Yuanye Bio-Technology (1 mentions)
Scopolamine scop, by Merck Group (1 mentions)
12 protocols using genistin
1
Soybean Fermentation with R. oligosporus
2
Soy Isoflavones as Alzheimer's Therapy
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Soy isoflavones (SI) (purify > 80% by HPLC) was purchased from Yuanye Biological Technology Co., Ltd. (Shanghai, China) and its chemical composition mainly included genistin 53.82%, daidzin 15.49% and glycitin 12.63%. Scopolamine (SCOP) was purchased from Sigma Chemical Co., Ltd. (St. Louis, MO, USA) and donepezil hydrochloride (Aricept) (DNP) was obtained from Eisai (Ibaraki, Japan). Carboxy methyl cellulose sodium (CMC-Na) was purchased from Shenggong Biological Engineering Co., Ltd. (Shanghai, China). Acetylcholinesterase (AChE), choline acetyltransferase (ChAT), malondialdehyde (MDA) and glutathione (GSH) commercial kits were obtained from Jiancheng Biological Technology Co., Ltd. (Nanjing, Jiangsu, China).
3
Phytochemical Analysis of Pueraria Extracts
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Folin-Ciocalteu's reagent, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), 2,2′-Azobis(2-methylpropionamidine) dihydrochloride (AAPH), fluorescein sodium salt, 2′,7′-dichlorofluorescin diacetate (DCFH-DA), Tris(hydroxymethyl)methyl aminomethane, β-Nicotinamide Adenine Dinucleotide (β-NAD), β-Mercatoethanol (β-Me) and α-D-glucopyranoside (pNPG) were obtained from Sigma-Aldrich (St. Louis, MO, USA). The enzymes used, including alcohol dehydrogenase (ADH) from saccharomyces cerevisiae, potassium-activated aldehyde dehydrogenase (ALDH) from yeast, α-glucosidase from saccharomyces cerevisiae, and α-amylase from porcine pancreas were all purchased from Sigma-Aldrich (St. Louis, MO, USA). Standard phenolic compounds including 3′-hydroxypuerarin, 6″-O-xylosidepuerarin, puerarin, mirificin, 3″-methyoxypuerarin, daidzin, rutin, ferulic acid, genistin, phlorizin, ononin, daidzein, genistein, quercitrin, and p-coumaric acid were obtained from Yuanye Biotechnology Co., Ltd. (Shanghai, China). HPLC-grade acetic acid and acetonitrile were purchased from Fisher Scientific (Waltham, MA, USA). All other reagents used were of analytical grade.
4
Phenolic Compound Extraction and Characterization
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Gallic acid, p-hydroxybenzoic acid, ferulic acid, trans-cinnamic acid, p-coumaric acid, benzoic acid, ellagic acid, protocatechuic acid, sinapic acid, caffeic acid, quercetin, rutin, taxifolin, isoquercitrin, catechin, gallocatechin, epicatechin, vitexin, genistin, daidzin, phloroglucinol, quinic acid, and 3,4-dihdyroxyphenylaceticacid were obtained from Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Procyanidin B1, procyanidin B2, and kaempferol-3-O-rutinoside were obtained from Chengdu Pusi Biotechnology Co., Ltd. (Chengdu, China). 2,20-azinobis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,4,6tri(pyridin-2-yl)-1,3,5-triazine (TPTZ), (±)-6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), fluorescein, 3′,6′-dihydroxyspiro [isobenzofuran-1 (3 H),9′-(9 H) xanthene]-3-one (FL), 2,2′-azobis (2-amidinopropane) dihydrochloride (AAPH), and EDTA-2Na were purchased from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Thermo Fisher Scientific (Waltham, MA, USA) provided the methanol (HPLC quality), formic acid (99.9%, LC-MS grade), and acetonitrile (LC-MS grade). Cellulase (T richoderma Vride G, 400 u/mg), hemicellulase (Aspergillus niger, 20 u/mg), and pectinase (Aspergillus niger, 500 u/mg) were obtained from Yuanye Bio-Technology Co., Ltd. (Shanghai, China).
5
Comprehensive Analysis of Fructus Puerariae
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A total of 13 batches of FP were collected from Zhejiang (S1), Guangdong (S2 and S3), Guangxi (S4, S5 and S6), Hebei (S8), Hunan (S9, S10, S11 and S12) and Sichuan (S13) provinces of China. Besides, the control herbs (S7) were purchased from the National Institutes for Food and Drug Control (Beijing, China). Reference standards (purity >98%) including rutin, daidzin, daidzein, and quercetin were obtained from the National Institutes for Food and Drug Control (Beijing, China). Glycitin, genistin, tectoridin, glycitein, genistein, tectorigenin, and biochanin A with the purities over 98% were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Nicotiflorin and ononin with the purities over 98% were bought from Chengdu Alfa Biotechnology Co., Ltd. (Chengdu, China). Meanwhile, formononetin and puerarin were bought from Chengdu Must Bio-Technology Co., Ltd.(Chengdu, China). HPLC grade acetonitrile (ACN) and methanol (MeOH) were obtained from Fisher Scientific (Fair Lawn, NJ, USA). Formic acid of HPLC grade was provided by Kermel Chemical Reagent Co., Ltd. (Tianjin, China) and pure water was obtained from Wahaha (Hangzhou, China). All the other reagents were of analytical grade.
6
Extraction and Quantification of Astragalus Compounds
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Radix astragali was purchased from Hunan Sanxiang Chinese Medicine Pieces Co., Ltd. (Changsha, China) BSA and glutaraldehyde (25% w/v aqueous solution) was purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Acetonitrile was HPLC grade from Tedia Company Inc. (Phoenix, AZ, USA). Ultrapure water (18.2 MΩ·cm resistivity) was obtained from a Milli-Q water purification system (Millipore, Bedford, MA, USA). All of the other chemicals were analytical grade and purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Genistin, calycosin-7-O-β-d -glucoside, ononin and formononetin were purchased from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China). The purity of each compound was determined to be ≥98% by HPLC analysis.
7
Isoflavone Mixture Effects on PC12 Cells
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An SI mixture containing 51.81% daidzin, 19% glycitin, 7.94% genistin, 2.45% daidzein, 0.81% glycitein, and 0.52% genistein, and their standards, were obtained from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China) [15 (link)]. Penicillin/streptomycin, fetal bovine serum (FBS), and Dulbecco’s modified Eagle medium (DMEM) were purchased from Gibco (CA, USA). PC12 cell lines were purchased from Nanjing Keygen Biotech. Co., Ltd. (Nanjing, China). The kits for ROS, apoptosis, the cell cycle, and the protein content were obtained from the Shanghai Beyotime Institute of Biotechnology (Shanghai, China). Antibodies for β-tubulin, Bax, cyclin A, p21, Bcl-xL, hypoxia-inducible factor-1 alpha (HIF-1α), LC3, p62, Nrf2, p-Akt, Akt, p-mTOR, mTOR, p-p38 MAPK, p38 MAPK, and LamB were obtained from Shanghai Abmart Inc. (Shanghai, China).
8
UPLC-MS/MS Analysis of Isoflavonoids
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An Ultimate 3000 Ultra High Performance Liquid Chromatography (UPLC) system coupled with LTQ-Orbitrap velos pro mass spectrometer was used (Thermo-Fisher, Waltham, MA, USA), as well as an Acquity UPLC I-Class UPLC system coupled with Xevo TQ-S micro triple quadrupole mass spectrometer (Waters, Milford, MA, USA).
The reference compounds of formononetin, ononin, daidzein, daidzin, genistin, genistein, calycosin-7-O-β-d -glucoside, biochanin A, and chlorogenic acid (purity > 98% for all) were all purchased from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China). Acetonitrile and formic acid were purchased from Fisher Scientific, San Jose, CA, USA Company (mass spec-grade). Deionized water was prepared by Milli-Q ultrapure water preparation system (Millipore Co., Ltd., Billerica, MA, USA). All other chemicals were purchased from Beijing Chemical Works (Beijing, China, analytical grade).
The reference compounds of formononetin, ononin, daidzein, daidzin, genistin, genistein, calycosin-7-O-β-
9
Extraction and Purification of Soybean Compounds
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The stachyose (pure>80%) was extracted from soybean residue [22 (link)]. Soybean isoflavones (pure>95%) includes 50% of daidzin, 6% of daidzein, 26% of glycitin, 3% of glycitein, 10% of genistin and 2% of genistein for animal experiments and the analytical standards of daidzein, glycitein and genistein (pure>98%) were bought from Shanghai Yuanye Biotechnology co., Ltd. (Shanghai, China). Assay kits of total cholesterol (TC), total triglyceride (TG), high-densitylipoprotein-cholesterol (HDL-C), low-density lipoprotein-cholesterol (LDL-C), glutamic-oxal(o)acetic transaminase (GOT) and glutamicpyruvic transaminase (GPT) were purchased from HuiliBiotechnology Co. Ltd. (Changchun, China). Additionally, enzyme linked immunosorbent assay ELISA kits of interleukin-1 (IL-1), tumor necrosis factor (TNF-α), nuclear factor kappa-B (NF-κB), acetyl CoA carboxylase (ACC), fatty acid synthetase (FAS), glucose, insulin, leptin and lipopolysaccharide (LPS) were purchased from Jiancheng Bioengineering Institute (Nanjing, China). All other reagents and chemicals were analytical grade or higher.
10
Fatty Acid and Sterol Profiling
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The sesame oil, rapeseed oil, and soybean oil were purchased from local markets (Wuhan, Hubei, China). Before being used for analysis, all samples were stored in the dark. The standard samples of fatty acid methyl ester (FAME), brassicasterol, ergosterol, campesterol, campestanol, stigmasterol, β-sitosterol, Δ5-avenasterol, cycloartanol, cycloartenol, 24-methylene-cycloartenol, and cholestanol were purchased from the Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Daidzin, daidzein, genistin, and genistein were purchased from the Shanghai Yuanye Biotechnology Co. (Shanghai, China). Methyl sterculate (C20H36O2, Purity ≥ 98 %) and methyl malvalate (C19H34O2, purity ≥ 95 %) were purchased from the Matreya Lipid and Biochemistry Technology Co., Ltd., USA. All solvents used were of analytical grade, and all standards were stored at −20 °C in darkness.
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