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Slc9a1 sirna

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Slc9a1 siRNA is a small interfering RNA (siRNA) molecule designed to target and silence the expression of the Slc9a1 gene. The Slc9a1 gene encodes the sodium/hydrogen exchanger 1 (NHE1) protein, which plays a role in cellular pH regulation. The Slc9a1 siRNA can be used to study the function of the Slc9a1 gene and the NHE1 protein in various experimental models.

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Lab products found in correlation

Accell sirna delivery medium, by Horizon Discovery (2 mentions) Acell smart pool of sirna targeting mouse, by Horizon Discovery (2 mentions) Alpha enac scnn1a, by Horizon Discovery (2 mentions)

Close spelling variants of this product

SiRNAs

2 protocols using slc9a1 sirna

1

Splenic DCs siRNA Knockdown Assay

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Mouse splenic DCs were isolated using MACS. Four million cells were re-suspended in 1 mL of Accell siRNA delivery medium (GE Healthcare Dharmacon) supplemented with 2.5% FBS, 1 mM sodium pyruvate and 25 mM HEPES. Cells were incubated for 72 hr with 1 µM Acell SMART pool of siRNA targeting mouse NHE1 (Slc9a1 siRNA [E-048336-00-0005], or Alpha ENaC (Scnn1a [E-040678-00-0005] GE Healthcare Dharmacon). ON-TARGETplus Non-targeting Control siRNA (D-001810-01-05) was used as a negative control. Sodium chloride (40 mM) was added for additional 24 hr. The knockdown levels of Alpha ENaC and NHE1 were analyzed by western blot.
Barbaro N.R., Foss J.D., Kryshtal D.O., Tsyba N., Kumaresan S., Xiao L., Mernaugh R.L., Itani H.A., Loperena R., Chen W., Dikalov S., Titze J.M., Knollmann B.C., Harrison D.G, & Kirabo A. (2017). Dendritic Cell Amiloride-Sensitive Channels Mediate Sodium-Induced Inflammation and Hypertension. Cell reports, 21(4), 1009-1020.
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2

Splenic DCs siRNA Knockdown Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse splenic DCs were isolated using MACS. Four million cells were re-suspended in 1 mL of Accell siRNA delivery medium (GE Healthcare Dharmacon) supplemented with 2.5% FBS, 1 mM sodium pyruvate and 25 mM HEPES. Cells were incubated for 72 hr with 1 µM Acell SMART pool of siRNA targeting mouse NHE1 (Slc9a1 siRNA [E-048336-00-0005], or Alpha ENaC (Scnn1a [E-040678-00-0005] GE Healthcare Dharmacon). ON-TARGETplus Non-targeting Control siRNA (D-001810-01-05) was used as a negative control. Sodium chloride (40 mM) was added for additional 24 hr. The knockdown levels of Alpha ENaC and NHE1 were analyzed by western blot.
Barbaro N.R., Foss J.D., Kryshtal D.O., Tsyba N., Kumaresan S., Xiao L., Mernaugh R.L., Itani H.A., Loperena R., Chen W., Dikalov S., Titze J.M., Knollmann B.C., Harrison D.G, & Kirabo A. (2017). Dendritic Cell Amiloride-Sensitive Channels Mediate Sodium-Induced Inflammation and Hypertension. Cell reports, 21(4), 1009-1020.
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