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MEIS1 is a laboratory equipment product offered by Thermo Fisher Scientific. It is a specialized instrument designed for scientific research and analysis purposes. The core function of MEIS1 is to provide precise and reliable measurements or data for researchers and scientists, but a detailed description of its intended use or capabilities cannot be provided while maintaining an unbiased and factual approach.

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Lab products found in correlation

Hs00153408 m1, by Thermo Fisher Scientific (2 mentions) Hoxa9, by Thermo Fisher Scientific (2 mentions) Hs00365956 m1, by Thermo Fisher Scientific (2 mentions) Hs00180020 m1, by Thermo Fisher Scientific (2 mentions) Bcl 2, by Thermo Fisher Scientific (1 mentions) Dnmt3a, by Thermo Fisher Scientific (1 mentions)

3 protocols using meis1

1

In Vivo Evaluation of Compound Efficacy Against MV4;11 Tumor Xenografts

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Female Balb/c nude mice (6–8 weeks, Beijing Anikeeper Biotech. Ltd) were inoculated subcutaneously on the right flank with MV4;11 tumor cells (1 × 107) in 0.1 mL of 1:1 v/v of IMDM:Matrigel for tumor development. Compound treatment was initiated when the mean tumor volume reached about 300 to 400 mm3. Mice were randomly assigned to respective treatment groups such that the average starting tumor size and body weight were the same for each treatment group. Mice were dosed with vehicle (5% DMSO:95% of 10% w/v Kleptose HPB, pH 4.0) or compound at 100 or 200 mg/kg p.o., b.i.d. at 12 hours intervals. For PD readouts (tumor volume and gene analysis), five animals per group were used. Animals were checked daily and body weights measured every 2 days throughout the study. Tumor volume was estimated using the formula: TV = a × b2/2 throughout the study, where “a” and “b” are the long and short diameters of a tumor, respectively. Individual tumor samples for gene analysis were taken at 2 hours after dose 8 days of dosing, and the following genes were analyzed: MYC (Hs00153408_m1, Thermo Fisher), HOXA9 (Hs00365956_m1, Thermo Fisher), MEIS1 (Hs00180020_m1, Thermo Fisher), and MYB (see Supplementary Table S4).
Liu Y., Li Q., Alikarami F., Barrett D.R., Mahdavi L., Li H., Tang S., Khan T.A., Michino M., Hill C., Song L., Yang L., Li Y., Pokharel S.P., Stamford A.W., Liverton N., Renzetti L.M., Taylor S., Watt G.F., Ladduwahetty T., Kargman S., Meinke P.T., Foley M.A., Shi J., Li H., Carroll M., Chen C.W., Gardini A., Maillard I., Huggins D.J., Bernt K.M, & Wan L. (2022). Small-Molecule Inhibition of the Acyl-Lysine Reader ENL as a Strategy against Acute Myeloid Leukemia. Cancer Discovery, 12(11), 2684-2709.
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2

Balb/c Nude Mice Xenograft Model

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Female Balb/c nude mice (6–8 weeks, Beijing Anikeeper Biotech. Ltd) were inoculated subcutaneously on the right flank with MV4–11 tumor cells (1 × 107) in 0.1 mL of 1:1 v/v of IMDM: Matrigel for tumor development. Compound treatment was initiated when the mean tumor volume reaches about 300~400 mm3. Mice were randomly assigned to respective treatment groups such that the average starting tumor size and body weight was the same for each treatment group. Mice were dosed with vehicle (5% DMSO & 95% (10% HPB in water) v/v adjusted to pH4.0) or compound at 100 or 200mg/kg P.O., BID at 12 hours intervals. For PD readouts (tumor volume ad gene analysis), 5 animals per group were used. Animals were checked daily and body weights measure every 2 days throughout the study. Tumor volume was estimated using the formula: TV = a × b2/2 throughout the study, where “a” and “b” are the long and short diameters of a tumor, respectively. Individual tumor samples for gene analysis were taken at 2 hours post-dose 8 days of dosing and the following genes were analyzed: MYC (Hs00153408_m1, ThermoFisher), HOXA9 (Hs00365956_m1, Thermofisher), MEIS1 (Hs00180020_m1, Thermofisher), MYB (see Supplementary Table S4).
Liu Y., Li Q., Alikarami F., Barrett D.R., Mahdavi L., Li H., Tang S., Khan T.A., Michino M., Hill C., Song L., Yang L., Li Y., Pokharel S.P., Stamford A.W., Liverton N., Renzetti L.M., Taylor S., Watt G.F., Ladduwahetty T., Kargman S., Meinke P.T., Foley M.A., Shi J., Li H., Carroll M., Chen C.W., Gardini A., Maillard I., Huggins D.J., Bernt K.M, & Wan L. (2022). Small-Molecule Inhibition of the Acyl-Lysine Reader ENL as a Strategy against Acute Myeloid Leukemia. Cancer Discovery, 12(11), 2684-2709.
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3

Gene Expression Analysis in Tumors

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RNA and cDNA were prepared from tumors and expression analysis performed using standard procedures with the following probes: DNMT3A (Hs01027166), HoxA11 (Hs00194149), Meis1 (Hs01017441), Bcl2 (Hs00236329), MYC (Hs00153408, all Thermo Fisher) with ABL as control.
Bäumer N., Scheller A., Wittmann L., Faust A., Apel M., Nimmagadda S.C., Geyer C., Grunert K., Kellmann N., Peipp M., Kailayangiri S., Gutierrez Suburu M.E., Strassert C.A., Schenk M., Greune L., Rüter C., Dersch P., Hartmann W., Rossig C., Neri D., Müller-Tidow C., Schwöppe C., Schliemann C., Khandanpour C., Lenz G., Berdel W.E, & Bäumer S. (2022). Electrostatic anti-CD33-antibody–protamine nanocarriers as platform for a targeted treatment of acute myeloid leukemia. Journal of Hematology & Oncology, 15, 171.
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