Amersham ecl chemiluminescence system

Manufactured by GE Healthcare

Sourced in United Kingdom

The Amersham ECL chemiluminescence system is a laboratory equipment used for the detection and quantification of proteins in Western blot analysis. It utilizes chemiluminescent substrates to generate a light signal that is proportional to the amount of target protein present in the sample.

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Lab products found in correlation

β actin, by Merck Group (3 mentions) Hybond polyvinylidene difluoride transfer membranes, by GE Healthcare (2 mentions) Hybond polyvinylidene difluoride membrane, by GE Healthcare (1 mentions) Hybond p, by GE Healthcare (1 mentions) α sma, by Abcam (1 mentions) Horseradish peroxidase conjugated antibodies against rabbit igg or mouse igg, by GE Healthcare (1 mentions) E cadherin, by Cell Signaling Technology (1 mentions) Vimentin, by Cell Signaling Technology (1 mentions) Anti β actin antibody, by Merck Group (1 mentions)

Close spelling variants of this product

ECL system (381 citations) Amersham ECL (119 citations) Chemiluminescence system (44 citations) ECL chemiluminescence (39 citations) ECL chemiluminescence system (32 citations) Amersham ECL system (12 citations) AmershamTM ECLTM (3 citations) Amersham ECL chemiluminescence detection system (2 citations) Amersham enhanced chemiluminescence (ECL) detection system (2 citations)

5 protocols using amersham ecl chemiluminescence system

Western Blot Protein Analysis

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Proteins extracted from cells were electrophoresed on 10% SDS-polyacrylamide gels and transferred to Hybond-polyvinylidene difluoride membranes (GE Healthcare UK Ltd., Amersham, UK). The membranes were incubated overnight at 4 °C with primary antibodies against AIF (1:1000, 5318; Cell Signaling Technology, Danvers, MA, USA), E1A (1:1000, 554155; BD Pharmingen, San Jose, CA, USA), p53 (1:1000, 18032; Cell Signaling Technology), and β-actin (1:5000, A-5441; Sigma-Aldrich, St. Louis, MO, USA), followed by incubation with secondary antibodies for 1 h at room temperature. The Amersham ECL chemiluminescence system (GE Healthcare UK Ltd.) was used to detect peroxidase activity of the bound antibody.

Hashimoto M., Kuroda S., Kanaya N., Kadowaki D., Yoshida Y., Sakamoto M., Hamada Y., Sugimoto R., Yagi C., Ohtani T., Kumon K., Kakiuchi Y., Yasui K., Kikuchi S., Yoshida R., Tazawa H., Kagawa S., Yagi T., Urata Y, & Fujiwara T. (2024). Long-term activation of anti-tumor immunity in pancreatic cancer by a p53-expressing telomerase-specific oncolytic adenovirus. British Journal of Cancer, 130(7), 1187-1195.

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Adenovirus E1A Protein Detection

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Proteins extracted from whole-cell lysates were electrophoresed on 10 % SDS–polyacrylamide gels and were transferred to Hybond-polyvinylidene difluoride transfer membranes (GE Healthcare UK Ltd.). The membranes were incubated with primary antibodies against adenovirus type 5 E1A (BD Biosciences, Franklin Lakes, NJ) and β-actin (Sigma-Aldrich), followed by peroxidase-linked secondary antibody. The Amersham ECL chemiluminescence system (GE Healthcare UK Ltd.) was used to detect the peroxidase activity of the bound antibody. Equal loading of samples was confirmed by β-actin analysis.

Aoyama K., Kuroda S., Morihiro T., Kanaya N., Kubota T., Kakiuchi Y., Kikuchi S., Nishizaki M., Kagawa S., Tazawa H, & Fujiwara T. (2017). Liposome-encapsulated plasmid DNA of telomerase-specific oncolytic adenovirus with stealth effect on the immune system. Scientific Reports, 7, 14177.

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Western Blot Protein Detection

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Proteins were extracted from whole-cell lysates or nuclear proteins, electrophoresed on polyacrylamide gels and transferred onto membranes. The membranes were incubated with primary antibodies overnight at 4 °C, followed by secondary antibodies 60 min at RT, and then visualized using the Amersham ECL chemiluminescence system (GE Healthcare, IL, USA). Equal loading of the samples was confirmed using β-actin.

Nishiwaki N., Noma K., Ohara T., Kunitomo T., Kawasaki K., Akai M., Kobayashi T., Narusaka T., Kashima H., Sato H., Komoto S., Kato T., Maeda N., Kikuchi S., Tanabe S., Tazawa H., Shirakawa Y, & Fujiwara T. (2023). Overcoming cancer-associated fibroblast-induced immunosuppression by anti-interleukin-6 receptor antibody. Cancer Immunology, Immunotherapy, 72(7), 2029-2044.

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Immunoblotting Analysis of Cellular Markers

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Cells were washed with cold phosphate-buffered saline (PBS) and lysed with the SDS buffer. Whole-cell lysates were loaded into each lane of an 8% SDS-polyacrylamide gel and electrophoretically transferred to polyvinylidene difluoride membranes (Hybond-P; GE Health Care, Buckinghamshire, UK). Membranes were incubated with primary antibodies against CD80 (EPR1157; Abcam, Cambridgeshire, UK), CD68 (EPR1392Y; Abcam), CD204 (MSR-A; TransGenic), E-cadherin (Cell Signaling Technology, Danvers, MA, USA), vimentin (Cell Signaling Technology), α-SMA (Abcam), and β-actin (Sigma-Aldrich) overnight at 4 °C. The secondary antibodies used were horseradish peroxidase-conjugated antibodies against rabbit IgG or mouse IgG (GE Healthcare). The blots were visualized using an Amersham ECL chemiluminescence system (GE Healthcare) according to the manufacturer’s protocol.

Kuwada K., Kagawa S., Yoshida R., Sakamoto S., Ito A., Watanabe M., Ieda T., Kuroda S., Kikuchi S., Tazawa H, & Fujiwara T. (2018). The epithelial-to-mesenchymal transition induced by tumor-associated macrophages confers chemoresistance in peritoneally disseminated pancreatic cancer. Journal of Experimental & Clinical Cancer Research : CR, 37, 307.

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HER2-ECD Expression in Gastric Cancer Cells

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MKN1, MKN45 and N87 cells (2×10⁵ cells) were seeded into 6-well plates. Cells were washed with cold phosphate buffered saline (PBS) and lysed with the sodium dodecyl sulfate (SDS) buffer. Equivalent amounts of protein from whole cell lysates were loaded into each lane of an 8% SDS-polyacrylamide gel and electrophoretically transferred to Hybond-polyvinylidene difluoride transfer membranes (GE Healthcare UK Ltd.). Membranes were incubated with primary antibodies against HER2-ECD (Thermo Scientific., Yokohama, Japan) overnight at 4°C and visualized using an Amersham ECL chemiluminescence system (GE Healthcare UK Ltd.) according to the manufacturer’s protocol. Equal loading of samples was confirmed by stripping each blot and reprobing with anti-β actin antibody (Sigma-Aldrich). In this experiment, MKN1 and MKN45 cells were infected with Ad/HER2-ECD at a different multiplicity of infection (MOI) (0, 20, 50 and/or 100) for 24 and/or 48 h. Ad/GFP was used as a control adenovirus.

Ishida M., Kagawa S., Shimoyama K., Takehara K., Noma K., Tanabe S., Shirakawa Y., Tazawa H., Kobayashi H, & Fujiwara T. (2016). Trastuzumab-based photoimmunotherapy integrated with viral HER2 transduction inhibits peritoneally disseminated HER2-negative cancer. Molecular cancer therapeutics, 15(3), 402-411.

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