Anti chc

A-769662 was obtained from Abcam (Cambridge, MA), AICAR was obtained from Cell Signaling Technology (Danvers, MA). Sulfo-NHS-SS-biotin was obtained from Pierce (Thermo Fisher Scientific, Rockford, IL). Antibodies used for immunoblotting were as follows: anti-EGFR from Genetex (Irvine, CA), anti-CHC from Santa Cruz Biotechnology (Santa Cruz, CA), anti-pACC, anti-AMPK (α1/2), anti- actin, and anti-Erk from Cell Signaling Technology (Danvers, MA), and anti-ZNF142 antibodies from Aviva Systems Biology (San Diego, CA). Antibodies used for immunofluorescence microscopy were as follows: anti-β1-integrin from EMD Millipore, Darmstadt, Germany), and anti-TfR from Santa Cruz Biotechnology (Santa Cruz, CA).

Lysates were prepared with an NP40 buffer, as previously described [25 (link)]. We used a Tris-Acetate PAGE system to analyze the samples [26 (link)], with a gel documentation system (LAS-3000 Fujifilm). Antibodies used: anti-CHC (Santa Cruz Biotechnology #sc12734), anti-C-RAF (BD Biosciences #610151), anti-p-ERK1/2 (Sigma-Aldrich #M8159), anti-ERK1/2 (Cell Signaling #9102), anti-p-p38 (Cell Signaling #9211), anti-p38 (Santa Cruz Biotechnology #sc-535), anti-Vinculin (Santa Cruz Biotechnology #sc-25336), and anti-HERC1 (410) [27 (link)].