2 2 bipyridyl dip

Yeast cell culture and genetic manipulations were performed using standard methods^{60 (link)}. S. pombe strains used in this study are listed in Supplementary Table 1. Yeast extract rich medium supplemented with adenine (YEA) was used to grow cultures. Cells cultured at 18°C were grown to mid-log phase (OD₆₀₀ = 0.4–0.8) at 30°C and shifted to 18°C for 3 days. Cultures were maintained in early- to mid-log phase (OD₆₀₀ = 0.1–0.8) during 18°C growth. Cells cultured at 37°C were first grown at 26°C and shifted to 37°C for 5 hours. For iron chelation experiments, cultured cells were treated with 250µM 2,2’-bipyridyl (Dip) (Sigma) immediately before the shift to 18°C.