On weeks 3, 6, 10, 16, 25, and 33 after transplanting, stems, leaves and fruits were sampled, washed with distilled water, and then dried in a ventilated oven at 75°C to constant weight. Nutrient contents in leaves and fruits samples were analyzed. The contents of K, Ca, Mg, and P were assayed after digestion with H2SO4-HNO3-HClO4 (H2SO4:HNO3:HClO4 = 1 ml:5 ml:1 ml) by inductively coupled plasma spectrometry (ICPE-9000, Shimazu, Japan; ICP-MS 7900, Agilent Technologies, United States). The N content was assayed after digestion with H2SO4-H2O2 by continuous flowing analyzer (AA3, Seal, Germany). The S content was assayed after digestion with HNO3 by inductively coupled plasma spectrometry (ICP-MS 7900, Agilent Technologies, United States) (Zhou et al., 2000 ).
7900 icp ms
Manufactured by Agilent Technologies
Sourced in United States, Japan, Germany
The 7900 ICP-MS is an inductively coupled plasma mass spectrometer manufactured by Agilent Technologies. It is designed to perform high-sensitivity elemental analysis of a wide range of sample types. The 7900 ICP-MS uses a plasma source to ionize the sample and a mass spectrometer to separate and detect the ions, providing quantitative data on the elemental composition of the sample.
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Lab products found in correlation
Bca protein assay, by Thermo Fisher Scientific (4 mentions)
Multi element calibration standard 2a, by Agilent Technologies (3 mentions)
Sps 4 autosampler, by Agilent Technologies (3 mentions)
Micromist nebulizer, by Agilent Technologies (3 mentions)
Omni bead ruptor 24, by Qiagen (2 mentions)
Ceramic bead tubes, by Qiagen (2 mentions)
Optima grade, by Thermo Fisher Scientific (2 mentions)
Environmental calibration standard, by Agilent Technologies (2 mentions)
5100 icp oes, by Agilent Technologies (2 mentions)
Suprapur, by Merck Group (2 mentions)
Nanodrop 1000, by Agilent Technologies (2 mentions)
Icpe 9000, by Shimadzu (2 mentions)
Icap 6200 icp aes, by Thermo Fisher Scientific (1 mentions)
Nwr 193 laser, by Agilent Technologies (1 mentions)
Ethos x microwave, by Milestone (1 mentions)
Bovine serum albumin (bsa), by Thermo Fisher Scientific (1 mentions)
Specpure, by Thermo Fisher Scientific (1 mentions)
Amicon ultra 15, by Merck Group (1 mentions)
D max 2400, by Rigaku (1 mentions)
3flex analyzer, by Micromeritics (1 mentions)
98 protocols using 7900 icp ms
1
Nutrient Content Analysis of Transplanted Crops
2
Soil Heavy Metal Analysis Protocol
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The soil samples were processed according to the methods described in the Environmental Protection Standards of the People’s Republic of China (HJ 803-2016). The debris, such as sticks, leaves, and stones, was removed from the soil samples. The soil samples were subsequently air-dried, and roughly-ground and finely-ground to pass a sieve with a pore size of 0.15 mm (100 mesh). Then, 0.1 g of the sample was placed in a closed Teflon digestion tank, 4.5 mL hydrochloric acid and 1.5 mL nitric acid were added, and the samples were digested with a microwave digestion instrument (MARS-6, CEM). The concentrations of V, Cr, Mn, Co, Ni, Cu, Zn, As, Cd, Sb, and Pb were determined using inductively coupled plasma mass spectrometry (7900 ICP-MS, Agilent). Furthermore, Hg was analyzed using thermal decomposition, amalgamation, and atomic absorption spectrophotometry (Hydra-C, Leeman).
3
Tissue Metal and Heme Analysis
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Prior to metal and heme analyses, frozen tissues were added to three volumes of pure water and homogenized in ceramic bead tubes (Qiagen) using an Omni Bead Ruptor 24. For metal analysis, homogenate aliquots were digested overnight in 5:1 HNO3:H2O2, dried, and resuspended in 2% HNO3 for analysis using an Agilent 7900 ICP-MS. Calibration standard solutions for determination of Fe, Zn, Cu and Mn were prepared from Agilent multi-element calibration standard-2A. Protein concentrations of homogenates were determined by BCA Protein Assay (Thermo Fisher Scientific) for normalization.
4
Soil Elemental Analysis by ICP-MS
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An amount of 0.1–0.2 g soil was placed into a closed digestion tank of polytetrafluoroethylene, amounts of 6 mL hydrochloric acid (1.19 g/mL) and 2 mL nitric acid (1.42 g/mL) were then added before being digested with a microwave at 120, 150 and 185 °C for 2, 5 and 40 min respectively, and the total content of Mn, Fe and Al was determined using an inductively coupled plasma mass spectrometer (7900 ICP−MS, Agilent in Tokyo, Japan).
5
Elemental Analysis of Wines
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Major and minor elements of the wines were analyzed at the ICP-MS & XRF Unit of the Central Analytical Facility of the Stellenbosch University, South Africa, using a Thermo ICap 6200 ICP-AES for the major and minor elements down to mid-ppb values and an Agilent 7900 ICP-MS for the ultra-trace analysis. Data are quantified with calibration solutions prepared from NIST traceable standards, and quality control procedures according to US Environmental Protection Agency guidelines are followed to ensure accuracy of data [41 ].
6
Microwave-Assisted Elemental Analysis
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Digestions were carried out in Ethos™ Easy (Millestone, Sorisole, Italy), an advanced microwave digestion system. First, samples were defrosted and mixed in the digestion solution containing one part of concentrated nitric acid and two parts of water. Subsequently, the digestions were performed under a 200 °C cycle for 1.5 h. Thereafter, elemental cerium in tissue were analysed using ICP-MS (7900 ICPMS, Agilent, Santa Clara, CA, USA), in Chemical analysis service (UAB, Barcelona, Spain).
7
Elemental Analysis of SaTatD Crystals
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Inductively coupled plasma mass spectrometry (ICP-MS) was conducted to identify which metal ions were present in the crystal structure of SaTatD. A high concentration of the purified SaTatD protein (14 mg ml−1) and an abundance of SaTatD crystals dissolved in water were prepared and analyzed for the presence of Mg2+, Ca2+, Mn2+, Ni2+ and Zn2+. Before measurement, the samples were denatured in nitric acid using a microwave at 1200–1800 W. ICP-MS (7900 ICP-MS, Agilent, USA) and argon plasma (6000–8000 K) were used for detection. The radio-frequency power and matching were 1550 W and 1.72 V, respectively. All data are shown using the average of three independent experiments.
8
Microwave Digestion for Mercury Analysis
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For sample preparation, microwave digestion was
used. The exact weight of 0.20 g of each sample was transferred into
a microwave vessel. Then, 9.00 mL of nitric acid (conc. 65%, w/w)
and hydrogen peroxide (conc. 30% w/w) was added into the microwave
vessel as digestion reagents. Finally, under microwave digestion around
50 min, the sample solution was transferred into a volumetric flask,
and the volume was made up to 100 mL with deionized water. Afterward,
the prepared sample solutions were analyzed by ICP-MS. ICP-MS (7900
ICP-MS, Agilent, USA), with a detection limit of mercury of 0.001
mg L–1, was used as the standard method to validate
mercury analysis.
used. The exact weight of 0.20 g of each sample was transferred into
a microwave vessel. Then, 9.00 mL of nitric acid (conc. 65%, w/w)
and hydrogen peroxide (conc. 30% w/w) was added into the microwave
vessel as digestion reagents. Finally, under microwave digestion around
50 min, the sample solution was transferred into a volumetric flask,
and the volume was made up to 100 mL with deionized water. Afterward,
the prepared sample solutions were analyzed by ICP-MS. ICP-MS (7900
ICP-MS, Agilent, USA), with a detection limit of mercury of 0.001
mg L–1, was used as the standard method to validate
mercury analysis.
9
Hair Analysis Using Laser Ablation ICP-MS
10
ICP-MS-Based Electrochemical Analysis Protocol
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Measurements
were performed using an Agilent 7900 ICP-MS. The ICP-MS
uses a collision cell with 5 mL/min flow of helium as cell gas. External
calibration was performed with multielement standard solutions provided
by Agilent and Inorganic Ventures. Downstream of the electrochemical
cell the analyte was mixed with internal standard solutions containing
cobalt having a similar mass as zinc.
A scheme of the setup
is shown inFigure 1 . The in-house built electrochemical flow cell was made out of PTFE.
The cell is illuminated by a UV LED (UV5TZ-390-15, Bivar, Inc.) shining
through a quartz rod from the top on the active working electrode
area. The illuminated area is at least 3.14 mm2 (2 mm diameter
rod) neglecting any scattering of UV light. Total electrode area is
about 7 mm2 (3 mm diameter O-ring). Pressurized air was
used for pumping the electrolyte with a stable and pulsation free
laminar flow. Flow was monitored by measuring with an in-flow pressure
sensor and by weighing the collected waste electrolyte. The flow is
set to 6 ± 0.4 mg of solution per second. Before each experiment
the electrolyte was purged with compressed and filtered air for at
least 30 min to guarantee the same concentration of dissolved oxygen.
All electrochemical experiments were done using a Ag|AgCl-electrode
as reference and presented data is referenced to that potential.
were performed using an Agilent 7900 ICP-MS. The ICP-MS
uses a collision cell with 5 mL/min flow of helium as cell gas. External
calibration was performed with multielement standard solutions provided
by Agilent and Inorganic Ventures. Downstream of the electrochemical
cell the analyte was mixed with internal standard solutions containing
cobalt having a similar mass as zinc.
A scheme of the setup
is shown in
The cell is illuminated by a UV LED (UV5TZ-390-15, Bivar, Inc.) shining
through a quartz rod from the top on the active working electrode
area. The illuminated area is at least 3.14 mm2 (2 mm diameter
rod) neglecting any scattering of UV light. Total electrode area is
about 7 mm2 (3 mm diameter O-ring). Pressurized air was
used for pumping the electrolyte with a stable and pulsation free
laminar flow. Flow was monitored by measuring with an in-flow pressure
sensor and by weighing the collected waste electrolyte. The flow is
set to 6 ± 0.4 mg of solution per second. Before each experiment
the electrolyte was purged with compressed and filtered air for at
least 30 min to guarantee the same concentration of dissolved oxygen.
All electrochemical experiments were done using a Ag|AgCl-electrode
as reference and presented data is referenced to that potential.
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