The total glutathione/oxidized glutathione assay kit (Nanjing JianCheng, Nanjing, China) was used to detect expression GSSG (oxidized glutathione, GSSG), GSH (reduced glutathione), and the GSH/GSSG ratio per the manufacturer's protocol.
Total glutathione oxidized glutathione assay kit
Manufactured by Nanjing Jiancheng
Sourced in China
The Total Glutathione/Oxidized Glutathione Assay Kit is a colorimetric assay designed to measure the total glutathione (reduced and oxidized forms) and oxidized glutathione levels in biological samples. The kit provides a simple, reliable, and sensitive method for the quantitative determination of these important antioxidants.
Automatically generated - may contain errors
Lab products found in correlation
Reduced glutathione gsh assay kit, by Nanjing Jiancheng (2 mentions)
Cysteine content test kit, by Nanjing Jiancheng (2 mentions)
Reduced glutathione assay kit, by Nanjing Jiancheng (2 mentions)
Glutathione peroxidase assay kit, by Abcam (1 mentions)
Malondialdehyde mda assay kit, by Nanjing Jiancheng (1 mentions)
Tissue iron assay kit, by Nanjing Jiancheng (1 mentions)
Lipid peroxidation mda assay kit, by Beyotime (1 mentions)
9 protocols using total glutathione oxidized glutathione assay kit
1
Assessing Glutathione Redox State
2
Quantifying Oxidative Stress Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The GSH/GSSG ratio, GSH levels and cysteine levels were detected by Reduced glutathione (GSH) assay kit (Nanjing jiancheng, China), Total glutathione / Oxidized glutathione assay kit (Nanjing jiancheng, China) and Cysteine content test kit (Nanjing jiancheng, China) on the basis of the manufacturer’s instructions. The transfected cells were lysed in culture dishes containing a lysis buffer, and 0.5 ml supernatant was taken from the resulting lysates which were centrifuged. Two ml of the application solution was added and mixed evenly, and then centrifuged at 3000 g for 10 min. Lastly 1 ml of the supernatant was taken for color reaction. The measurements were conducted with a UV–visible spectrophotometer.
A detailed description of the materials and methods used in this study can be found in the online supplementary material.
A detailed description of the materials and methods used in this study can be found in the online supplementary material.
3
Oxidative Stress Biomarker Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
An Iron Assay Kit (ab83366, Abcam), malondialdehyde (MDA) Assay Kit (Jiancheng, China), total glutathione/oxidized glutathione assay kit (Jiancheng, China), and Glutathione Peroxidase Assay Kit (ab102530, Abcam) were used to determine the relative levels of iron content, MDA, GSH, and GPX4 activity, respectively. All procedures were performed in accordance with the instructions.
4
Quantifying Sperm Glutathione Levels
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total glutathione (T-GSH) levels and glutathione (Oxidized) (GSSG) levels were evaluated with Total glutathione/Oxidized glutathione assay kit (A061-1-2, Nanjing Jiancheng Bioengineering Institute). Sperm samples were added to working solution IV and centrifuged at 3500× g for 10 min after ultrasonication on ice. For the detection of sperm T-GSH content, working solutions I, II, III were added to the supernatant sample, and the absorbance was measured with a microplate reader (TECAN, Infinite M Nano, Männedorf, Switzerland) at 405 nm at 30 s and 10 min 30 s points. For measuring the GSSG content, supernatants were pretreated with working solutions V and VI, and incubated at 37 °C for 30 min; the subsequent processes were the same as for T-GSH content detection. GSH content was calculated according to the formula: GSH = T − GSH − 2 × GSSG.
5
Quantifying Oxidative Stress Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The levels of GSH and MDA in cell extracts were determined. The Reduced glutathione (GSH) was analyzed using the Reduced Glutathione Assay kit (Nanjing Jiancheng Bioengineering Institute; cat. no. A006), according to the manufacturer's instructions. Total glutathione/oxidized glutathione (GSH/GSSG) was determined using the Total Glutathione/Oxidized Glutathione Assay kit (Nanjing Jiancheng Bioengineering Institute; cat. no. A061). MDA levels were determined using a lipid peroxidation (MDA) test kit (Biovision, Inc.; cat. no. K739-100) according to the manufacturer's instructions.
6
Kidney Tissue Iron and Glutathione Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The iron concentration of kidney tissue was measured by tissue iron assay kit (A039-2-1, Nanjing Jiancheng Bioengineering, China) according to the manufacturer’s protocol. GSH/GSSG ratio of kidney tissue was determined using total glutathione/Oxidized glutathione assay kit (A061-2-1, Nanjing Jiancheng Bioengineering, China) according to the manufacturer’s instruction.
7
Glutathione Assay in Tissue Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tissue samples were prepared as described above. Reduced glutathione (GSH) was analyzed using the Reduced Glutathione Assay Kit (Nanjing Jiancheng Bioengineering Institute Cat#A006, China), according to the manufacturer’s instructions. Total glutathione/oxidized glutathione (GSH/GSSG) was determined using the Total Glutathione/Oxidized Glutathione Assay Kit (Nanjing Jiancheng Bioengineering Institute Cat#A061, China), following the manufacturer’s instructions.
8
Quantification of Oxidative Stress Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The concentrations of cysteine, MDA and GSH were assessed using Cysteine Colorimetric assay kit (Elabscience, China), Lipid Peroxidation MDA assay kit (Beyotime, China) and total glutathione/Oxidized glutathione assay kit (Nanjing Jiancheng Bioengineering Institute, China) according to the manufacturer's instructions.
9
Quantification of Cellular Redox Status
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The GSH/GSSG ratio, GSH levels and cysteine levels were detected by Reduced glutathione (GSH) assay kit (Nanjing jiancheng, China), Total glutathione / Oxidized glutathione assay kit (Nanjing jiancheng, China) and Cysteine content test kit (Nanjing jiancheng, China) on the basis of the manufacturer's instructions. The transfected cells were lysed in culture dishes containing a lysis buffer, and 0.5ml supernatant was taken from the resulting lysates which were centrifuged. 2ml of the application solution was added and mixed evenly, and then centrifuged at 3500rpm for 10min. Lastly 1ml of the supernatant was taken for color reaction. The measurements were conducted with a UV-visible spectrophotometer.
A detailed description of the materials and methods used in this study can be found in the online supplementary material.
A detailed description of the materials and methods used in this study can be found in the online supplementary material.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!