Purified RPP sample was prepared as previously described [18 (link)]. High-fat diets (45% fat) were purchased from Research Diets Inc. (New Brunswich, NJ, USA). Dimethylbiguanide hydrochloride (DMBG) was purchased from Sino-American Shanghai Squibb Pharmaceuticals Ltd. (Shanghai, China). STZ was purchased from Sigma-Aldrich (St. Louis, MO, USA). Commercial assay kits for creatinine (CRE), glycated serum protein (GSP), total cholesterol (TC), total triglycerides (TG), total protein (TP), hepatic glycogen (GC), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and malonaldehyde (MDA) were obtained from Nanjing Jiancheng Biological Engineering Institute Co., Ltd. (Nanjing, China).
Malonaldehyde
Manufactured by Nanjing Jiancheng
Sourced in China
Malonaldehyde is a chemical compound commonly used in laboratory settings. It is a colorless liquid with a characteristic odor. Malonaldehyde serves as a core component in various analytical and research applications, but a detailed description of its intended use would require more information to maintain an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
Gsh px, by Nanjing Jiancheng (2 mentions)
Streptozotocin (stz), by Merck Group (1 mentions)
Uric acid, by Nanjing Jiancheng (1 mentions)
Ultrasonic cell grinder, by Ningbo Scientz Biotechnology (1 mentions)
Ldl c, by Nanjing Jiancheng (1 mentions)
Antibodies against srebp 1c, by Santa Cruz Biotechnology (1 mentions)
Mouse anti β actin antibody, by Abcam (1 mentions)
T 500, by Solarbio (1 mentions)
Ascorbic acid, by Sinopharm (1 mentions)
Rhodamine 123, by Merck Group (1 mentions)
Testosterone, by Yuanye Bio-Technology (1 mentions)
Milli q water puri cation system, by Merck Group (1 mentions)
Nicotinamide adenine dinucleotide phosphate nadph, by Yuanye Bio-Technology (1 mentions)
Methanol, by Merck Group (1 mentions)
Alanine aminotransferase alt, by Nanjing Jiancheng (1 mentions)
High density lipoprotein cholesterol, by Nanjing Jiancheng (1 mentions)
Simvastatin tablets, by Merck Group (1 mentions)
Superoxide dismutase, by Nanjing Jiancheng (1 mentions)
Total cholesterol, by Nanjing Jiancheng (1 mentions)
L dopa, by Merck Group (1 mentions)
10 protocols using malonaldehyde
1
Purified RPP Sample Preparation and Analysis
2
Comprehensive Hormone and Metabolite Analysis
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Follicle-Stimulating Hormone, FSH (B03PZB), Growth Hormone, GH (B12PZB), Luteinizing Hormone, LH(B04PZB), Androgens,T (B10PZB) and cortisol, COR (COR-D10PZB) were purchased from Beijing North Biotechnology Research Institute Co., LTD.; Estradiol, E2(B05PZB) was purchased from Tianjin Union Medical Science and Technology Co., LTD.; Superoxide dismutase, SOD ((A001-1-2), Malonaldehyde, MDA(A003-1-2), Glutathione peroxidase, GSH-PX (A005-1-2), Catalase, CAT (A007-1-1), Immunoglobulin m, IGM (Jh-00015), Immunoglobulin g, IGG (Jh-00013) and Immunoglobulin a, IGA(Jh-00014) were purchased from Nanjing Jiancheng Biological Engineering Research Institute Co., LTD.; Glutamic-pyruvic transaminase, ALT (Alanine substrate method, B2008),Glutamic oxalacetic transaminase, AST (aspartic acid substrate method, B2009), Total protein, TP (biuret method, B2010), Albumin, ALB (bromocresol green method, B2011), alkaline phosphatase, ALP (NPP substrate-AMP buffer method, B2014), Urea nitrogen, UREA(Urease-glutamate dehydrogenase method, B2017), Serum creatinine, CRE (Creatine oxidase method, B2018), Uric acid, UA (uricase method, B2019), Glucose, GLU (Glucose oxidase method,. B2007) were purchased from Beijing Beijian•xinchuangyuan Biotechnology Co., LTD.
3
Cellular Antioxidant Evaluation Protocol
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At the end of treatment, the cells were scraped off using cell scrapers, and the supernatant was discarded after centrifugation at 1000× g for 10 min at room temperature. Next, 1 mL PBS was added to the cell precipitate for washing and the supernatant was centrifuged at 1000× g for 10 min to leave the cell precipitate. Then, the cell precipitate was washed twice. A total of 400 µL PBS was added to the cell precipitate and homogenized by blowing using a pipettor. The cells were crushed in an ice-water bath using an ultrasonic cell grinder (Scientz, Ningbo, China) with a power of 300 W, 3 s each sonication, 30 s intervals, and 5 repetitions. In the end, the supernatant was centrifuged at 12,000× g for 10 min at 4 °C, and the malonaldehyde (MDA), superoxide dismutase (SOD), glutathione (GSH), and catalase (CAT) were detected according to the instructions of commercial kits (Jiancheng, Nanjing, China). All reactions were repeated three times (n = 3).
4
Tilapia Metabolic Regulation Study
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Tilapias were purchased from Hainan Xiangtai Fishery Co., LTD (Hainan, China) and stored at −20 °C. High-fat feed was purchased from Hunan Slac Jingda Laboratory Animal Co., Ltd. (Changsha, China). Enzyme-linked immunosorbent assay kits for insulin (INS), adiponectin (ADPN), leptin (LEP), IL-6, tumor necrosis factor-alpha (TNF-α) and free fatty acids (FFAs) were acquired from Shanghai Enzyme-linked Biotechnology Inc. (Shanghai, China). Commercial kits for aminotransferase (ALT), aminotransferase (AST), TC, TG, HDL-C, LDL-C, glutathione (GSH), superoxide dismutase (SOD) and malonaldehyde (MDA) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Antibodies against SREBP-1c were obtained from Santa Cruz Biotechnology Inc. (CA, USA). Antibodies against AMP-activated protein kinase (AMPK), phospho-AMPK, peroxisome proliferator-activated receptor (PPARγ) and fatty acid synthase (FAS) were obtained from Cell Signaling Technology (Beverly, MA, USA). Mouse anti-β-actin antibody and goat anti-mouse lgG-HRP secondary antibody were obtained from Abcam Inc. (Cambridge, UK). All other chemicals and reagents used were of analytical grade.
5
Guava Leaves for Antioxidant and Metabolic Evaluation
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Guava leaves were obtained from Jiangmen Nanyue Guava farmer cooperatives (Guangdong, China). Leaves were dried at 60 °C, then pulverized and sieved (40 mesh) for the experiments.
1,1-Diphenyl-2-picrylhydrazyl (DPPH) was purchased from Shanghai Macklin Biochemical Co., Ltd. ABTS+ and Trolox were obtained from Aladdin Industrial Corporation (Shanghai, China). Ascorbic acid was purchased from Sinopharm chemical reagent Co., Ltd. (Shanghai, China). Dextran T-2000, T-500, T-70, T-40, and T-10 were purchased from Solarbio (Beijing, China). Acorbose was obtained from Sigma-Aldrich Co., Ltd. (St. Louis, MO, USA). Streptozocin was obtained from MP Biomedicals (Santa Ana, CA, USA). Assay kits for total cholesterol (TC), total triglycerides (TG), glycated serum protein (GSP), creatinine (CRE), total antioxidant capacity (T-AOC), total superoxide dismutase (T-SOD), and malonaldehyde (MDA) were purchased from Nanjing jiancheng Bioengineering Institute (Nanjing, China). The high fat diet consisted of 10% lard, 20% sucrose, 10% yolk powder, 0.5% sodium cholate, and 59.5% conventional feed, which was purchased from Jiangsu synergetic pharmaceutical bioengineering Co., Ltd. (Nanjing, China).
1,1-Diphenyl-2-picrylhydrazyl (DPPH) was purchased from Shanghai Macklin Biochemical Co., Ltd. ABTS+ and Trolox were obtained from Aladdin Industrial Corporation (Shanghai, China). Ascorbic acid was purchased from Sinopharm chemical reagent Co., Ltd. (Shanghai, China). Dextran T-2000, T-500, T-70, T-40, and T-10 were purchased from Solarbio (Beijing, China). Acorbose was obtained from Sigma-Aldrich Co., Ltd. (St. Louis, MO, USA). Streptozocin was obtained from MP Biomedicals (Santa Ana, CA, USA). Assay kits for total cholesterol (TC), total triglycerides (TG), glycated serum protein (GSP), creatinine (CRE), total antioxidant capacity (T-AOC), total superoxide dismutase (T-SOD), and malonaldehyde (MDA) were purchased from Nanjing jiancheng Bioengineering Institute (Nanjing, China). The high fat diet consisted of 10% lard, 20% sucrose, 10% yolk powder, 0.5% sodium cholate, and 59.5% conventional feed, which was purchased from Jiangsu synergetic pharmaceutical bioengineering Co., Ltd. (Nanjing, China).
6
Analytical Characterization of Slag and Waste Residues
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Copper concentrate slag, iron ore slag, copper and sulfur ore slag, and cobalt-nickel-manganese waste residue were all provided by Hangzhou Customs, China. Chromatographic grade methanol and Rhodamine-123 were purchased from Sigma-Aldrich (St. Louis, MO). Phenacetin (purity > 98%), testosterone (purity > 98%), and nicotinamide adenine dinucleotide phosphate (NADPH, purity > 98%) were procured from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China). Detection kits of lactate dehydrogenase (LDH), superoxide dismutase (SOD), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and malonaldehyde (MDA) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). All chemicals used were of high-performance liquid chromatography (HPLC) grade and were used as received without any further puri cation and were obtained from Sigma-Aldrich. All solutions were prepared with deionized water. Deionized water was prepared by a Milli-Q water puri cation system (Millipore, Bedford, MA, USA).
7
Apocynum venetum Flavonoids Modulate Lipid Metabolism
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Total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), superoxide dismutase (SOD), and malonaldehyde (MDA) test kits were purchased from Nanjing Jiancheng Bioengineering Institute, China. VD3 injection was purchased from Shanghai General Pharmaceutical, China. Simvastatin tablets were purchased from Merck, Hangzhou, China. Sodium cholate and cholesterol were purchased from Sigma, USA. The antibody of intercellular cell adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), E-selectin, fractalkine (FKN), spleen tyrosine kinase (SYK), and p38 mitogen-activated protein kinase (p38) were purchased from Abcam, USA. C-reactive protein (CRP), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) determination kits were purchased from Shanghai Meixin Biological Engineering, China. Apocynum venetum flavonoids (AVF, flavonoid content ≥72%) were purchased from Nanjing Jingzhu Biotechnology Co., Ltd., China.
8
Measuring Photosynthetic Pigments and MDA
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The seedlings at 30‐day post‐sowing in soil were used to measure MDA and photosynthetic pigment. The contents of chlorophyll a, chlorophyll b, and carotenoids were determined according to He et al. (2018 (link)). The contents of malonaldehyde (MDA) were measured following the manufacturer's instructions (Nanjing Jiancheng Bioengineering Institute, Nanjing, China).
9
Antioxidant Effects Assessment Protocol
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To estimate changes in antioxidant effects, the activity of the antioxidase, superoxide dismutase (SOD) was determined using xanthine oxidase methods, and the content of lipid peroxidation production, malonaldehyde (MDA), was measured using thiobarbituric acid methods, according to the manufacturer's protocol (both from Nanjing Jiancheng Bioengineering Institute, Nanjing, China).
10
Antioxidant activity of lotus seedpods
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Mature lotus seedpods of Nelumbo nucifera Gaertn. (cv.: Number 2 Wuhan plant) were harvested from Honghu District in Hubei province, China. Superoxide dismutase (SOD), malonaldehyde (MDA), and hydroxyproline assay kits were purchased from Nanjing Jiancheng Biology Engineering Institute (Nanjing, China). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), monophenolase, l -DOPA, and l -tyrosine were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Human foreskin fibroblast cell HFF-1 was purchased from the China Center for Type Culture Collection (CCTCC) (Wuhan, China).
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