Caov3
Manufactured by Korean Cell Line Bank
The CAOV3 is a cell line derived from human ovarian carcinoma. It is maintained in culture and available for purchase from the Korean Cell Line Bank.
Automatically generated - may contain errors
Lab products found in correlation
Hct116, by Korean Cell Line Bank (2 mentions)
Mcf 7, by Korean Cell Line Bank (2 mentions)
Skov3, by American Type Culture Collection (2 mentions)
Sw480, by Korean Cell Line Bank (1 mentions)
Sw620, by Korean Cell Line Bank (1 mentions)
Skov3, by Korean Cell Line Bank (1 mentions)
Raw 264, by Korean Cell Line Bank (1 mentions)
Hs578t, by Korean Cell Line Bank (1 mentions)
Ovcar3, by Korean Cell Line Bank (1 mentions)
Tov112d, by American Type Culture Collection (1 mentions)
Mcf 7, by Welgene (1 mentions)
Hct116, by Welgene (1 mentions)
Ca ov 3, by Welgene (1 mentions)
Panc 1, by Korean Cell Line Bank (1 mentions)
Panc 1, by Welgene (1 mentions)
Rpmi 1640, by Welgene (1 mentions)
Dulbecco modified eagle medium (dmem), by Welgene (1 mentions)
Pex lgr5, by GeneCopoeia (1 mentions)
Snu 8, by Korean Cell Line Bank (1 mentions)
Snu 119, by Korean Cell Line Bank (1 mentions)
5 protocols using caov3
1
Cell Culture Protocol: Diverse Cell Lines
2
Culturing Breast and Ovarian Cancer Cell Lines
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The human breast cancer cell lines, Hs578T, T47D, MCF-7, and ovarian cancer cell lines, OVCAR-3 and Caov-3, were purchased from the Korean Cell Line Bank (KCLB; Seoul, Republic of Korea). The human ovarian cancer cell line TOV-112D was from American Type Culture Collection (ATCC; Manassas, VA, USA). TOV-112D cells were transfected for stable expression of CLDN3 as described in previous study43 . TOV-112D cells was cultured in 1:1 mixture of Media199/MCDB medium (HyClone, Logan, UT, USA) containing 15% FBS, 100 unit/mL penicillin, and 100 μg/mL streptomycin. T47D, MCF-7, OVCAR-3 cells were cultured in RPMI-1640 medium (HyClone) supplemented with 10% fetal bovine serum (FBS; HyClone), 100 U/mL penicillin, and 100 μg/mL streptomycin. Caov-3 and Hs578T cells were cultured in Dulbecco's modified Eagle's medium (DMEM, HyClone) supplemented with 10% FBS (HyClone), 100 U/mL penicillin, and 100 μg/mL streptomycin. The cells were incubated at 37 °C in a humidified 5% CO2 atmosphere.
3
Cancer Cell Line Cultivation Protocol
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Human colorectal cancer (HCT116), breast cancer (MCF-7), cervical cancer (HeLa), ovarian cancer (CAOV3), pancreatic cancer (PANC-1), lung cancer (A549), and melanoma cancer (A375) cell lines were purchased from Korea Cell Line Bank (KCLB, Korea) or American Type Culture Collection (ATCC, USA). HCT116, MCF-7, A375, and A549 cells were cultured in RPMI-1640 (Welgene, Korea) supplemented with 10% fetal bovine serum (FBS; GIBCO, USA). HeLa, CAOV3, and PANC-1 were cultured in DMEM (Welgene, Korea) supplemented with 10% FBS. All cancer cells were incubated at 37°C in 5% CO2 humidified incubator.
4
Overexpression of LGR5 in HGSC Cell Lines
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Four human HGSC cell lines (CaoV-3, NIH OVCAR-3, SNU-8, and SNU-119) were purchased from the Korean Cell Line Bank (Seoul, Korea). Cells were cultured in RPMI 1640 medium (Welgene, Daegu, Korea) containing 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA) and 1% penicillin/streptomycin (Gibco) and maintained at 37 °C in a humidified incubator with 5% CO2. Full-length cDNA encoding LGR5 (pEX-LGR5) and control vector were purchased from GeneCopoeia (Rockville, MD). Cancer cells were seeded at 1 × 105 cells/well in 6-well plates after transfection with control vector or pEX-LGR5 (2.5 μg) using the Neon transfection system (Thermo Fisher Scientific). One or two days after transfection, the cells were subjected to real-time PCR, immunoblotting or functional assays.
5
Culturing Four Epithelial Ovarian Cancer Cell Lines
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Four human epithelial ovarian cancer (EOC) cell lines (SKOV3, CAOV3, A2780, and ES-2) were used in this study. The CAOV3 was obtained from the Korean Cell Line Bank (Seoul, Korea). The SKOV3, A2780, and ES-2 cells were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). The SKOV3 and ES-2 cells were grown with McCoy's 5A Medium (ATCC) containing 10% fetal bovine serum (FBS; Life Technologies, Grand Island, NY, USA), 1.5 mM L-glutamine, 100 U/mL penicillin, and 100 mg/mL streptomycin. CAOV3 was grown with Dulbecco's modified Eagle's medium (DMEM, Life Technologies) containing 10% FBS (Life Technologies), 100 U/mL penicillin and 100 mg/mL streptomycin. A2780 was grown with Roswell Park Memorial Institute medium 1640 (RPM I1640, Life Technologies) containing 10% FBS (Life Technologies), 25 mM HEPES (4-(2-hydroxyethyl)-1piperazineethanesulfonic acid), 100 U/mL penicillin, and 100 mg/mL streptomycin. Cells were grown in a 100 mm dish at 37°C and 5% CO 2 . The cell growth and morphology were routinely monitored.
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