Animals used in Edu labeling experiments were incubated in 10 mM EdU in NM for the desired time and then treated for IF as described. After the final set of PBTx washes EdU incorporation was visualized using the Click-iT™ EdU Imaging Kit with Alexa Fluor™ 488 or 647 (Thermo Fisher Scientific, C10337/C10337) following the manufacturer’s protocol. Samples were mounted and imaged as for IF.
Click it edu imaging kit with alexa fluor 488 or 647
Manufactured by Thermo Fisher Scientific
The Click-iT™ EdU Imaging Kit with Alexa Fluor™ 488 or 647 is a detection kit used to label and visualize newly synthesized DNA in cells. It utilizes a copper-catalyzed click reaction to incorporate a fluorescent dye into DNA during the EdU labeling process.
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Lab products found in correlation
2 protocols using click it edu imaging kit with alexa fluor 488 or 647
1
EdU Incorporation Visualization Protocol
2
EdU Incorporation Visualization in Cells
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Animals used in Edu labeling experiments were incubated in 10 mM EdU in NM for the desired time and then treated for IF as described. After the final set of PBTx washes, EdU incorporation was visualized using the Click-iT™ EdU Imaging Kit with Alexa Fluor™ 488 or 647 (Thermo Fisher Scientific, C10337/C10337) following the manufacturer’s protocol. Samples were mounted and imaged as for IF.
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