The transfected TC cells were seeded into 6-well plates and allowed to grow to 100% confluence at 37°C. Then, a 2-mm wide wound was created on the cell surface using a sterile plastic scriber. The cells were then cultured in serum-free medium. The width of the wounds was observed using an inverted optical microscope (DP27; Olympus Corporation; magnification, ×100) at 0 and 48 h, and then measured using Image-Pro Plus Analysis software (version 6.0; Media Cybernetics, Inc.).
Image pro plus analysis software
Manufactured by Media Cybernetics
Sourced in United States, Japan
Image-Pro Plus is an image analysis software developed by Media Cybernetics. It provides tools for capturing, processing, analyzing, and managing digital images.
Automatically generated - may contain errors
Lab products found in correlation
Prolong gold with dapi, by Thermo Fisher Scientific (2 mentions)
Bp 98a, by Softron (1 mentions)
Ba3638, by Wuhan Servicebio Technology (1 mentions)
Triphenyltetrazolium chloride, by Merck Group (1 mentions)
Triphenyl tetrazolium chloride (ttc), by Media Cybernetics (1 mentions)
Pierce supersignal, by Thermo Fisher Scientific (1 mentions)
Lsm 510, by Zeiss (1 mentions)
Fitc conjugated goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Prism 8, by GraphPad (1 mentions)
Matrigel, by BD (1 mentions)
Transwell plate, by Corning (1 mentions)
Eclipse e600 microscope, by Nikon (1 mentions)
Rat anti mouse cd68, by Bio-Rad (1 mentions)
Rat anti mouse cd31, by BD (1 mentions)
Anti icam 1 bbig i1, by R&D Systems (1 mentions)
Vcam 1 bbig v1, by R&D Systems (1 mentions)
Axiovert 35 microscope, by Zeiss (1 mentions)
Axiocam ccl camera, by Zeiss (1 mentions)
Trap staining kit, by Merck Group (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
74 protocols using image pro plus analysis software
1
Wound Healing Assay in TC Cells
2
Quantifying Aortic Plaque and Inflammation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The plaque en face area of the whole aortas and cross-sectional area of atherosclerotic plaque from aortic root were stained with Oil Red O (4 (link), 11 (link), 13 (link)). To detect target protein expressions, the immunohistochemical analysis was used in serial plaque sections from the aortic arch. Immunohistochemical analysis of CD68 polyclonal antibody (1:200; Boster Biological Technology, BA3638), CD3 monoclonal antibody (1:200; Servicebio, GB13014), and α–smooth muscle actin monoclonal antibody (1:2000; Servicebio, GB13044) were performed. The sections from the aortic arch were additionally stained with monoclonal anti–ICAM-1 BBIG-I1 (1:100) or monoclonal anti–VCAM-1 BBIG-V1 (1:200) (R&D Systems) and rat monoclonal anti-CD31 (1:100; ABclonal, ab24590) and mounted in Prolong Gold with DAPI (Life Technologies). Images were quantified using Image Pro Plus Analysis Software (Media Cybernetics).
Blood pressure was noninvasively measured in animals by the tail-cuff method (Softron BP-98A, Tokyo, Japan). Blood pressure values were averaged from three consecutive measurements under steady-state conditions. Food intake, fecal output, and lipid content in feces were measured as previously described (27 (link)).
Blood pressure was noninvasively measured in animals by the tail-cuff method (Softron BP-98A, Tokyo, Japan). Blood pressure values were averaged from three consecutive measurements under steady-state conditions. Food intake, fecal output, and lipid content in feces were measured as previously described (27 (link)).
3
Quantifying Cerebral Contusion After Traumatic Brain Injury
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
On the 4 th day after TBI, the anaesthetized rats were intracardially perfused with normal saline. Their brain tissues were removed, immersed in cold saline for 5 min, and sliced into 2.0-mm thick sections with a tissue slicer. Triphenyl tetrazolium chloride (TTC; Sigma-Aldrich, St. Louis, MO, USA) staining procedures were used to determine the extent of the cerebral contusion caused by a TBI, as detailed in a previous study [25] . The contusion volume, as revealed by negative TTC stains that indicated dehydrogenase-deficient tissue, was measured in each slice and summed using Image-Pro Plus analysis software (Media Cybernetics, Silver Spring, MD, USA).
The corrected contusion volume (CCV) was calculated using the following equation to avoid overestimating the contusion volume:
CCV=(CH-(IH-ICA)) x d where CH represents the area of the contralateral hemisphere in square millimetres, IH is the area of the ipsilateral hemisphere in square millimetres, ICA is the area of ipsilateral contusion in square millimetres, and d is the thickness of the slices (1 mm).
The corrected contusion volume (CCV) was calculated using the following equation to avoid overestimating the contusion volume:
CCV=(CH-(IH-ICA)) x d where CH represents the area of the contralateral hemisphere in square millimetres, IH is the area of the ipsilateral hemisphere in square millimetres, ICA is the area of ipsilateral contusion in square millimetres, and d is the thickness of the slices (1 mm).
4
Western Blot Analysis of Cell Fractions
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell lysates, extracted nuclei and cytoplasm were subjected to western blot analysis, as described previously.21 (link) Briefly, proteins was separated on 10–15% SDS-PAGE gels, and the separated proteins were then transferred to PVDF membranes. The protein blots were blocked with 5% milk and probed sequentially with specific primary antibodies and horseradish peroxidase-conjugated secondary antibodies. The detection of specific proteins on the blots was achieved with enhanced chemiluminescence (Pierce SuperSignal, Thermo Fisher Scientific Inc., Rockford, IL, USA), and the results were captured on X-ray films. Densitometric analysis was performed using the Image-Pro Plus analysis software (Media Cybernetics, Inc., Rockville, MD, USA).
5
Immunofluorescence Analysis of PKCθ in TG Neurons
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Immunofluorescence analysis was performed as described previously [26 (link)]. Briefly, TG neurons were treated with tyramine for 15 min and then fixed with PFA (4%) in phosphate-buffered saline (PBS) for 20 min. The cells were sequentially permeabilized, blocked, and incubated with an anti-PKCθ primary antibody (rabbit, 1:500, Cell Signaling Technology) for 16 h at 4 °C. After three washes in PBS, TG neurons were visualized with FITC-conjugated goat anti-rabbit IgG (Invitrogen, 1:600). Immunopositive signals were characterized using laser scanning confocal microscopy (Zeiss LSM 510, Germany) and analyzed with Image-Pro Plus analysis software (v.6.0; Media Cybernetics).
6
Analyzing Opioid Receptor Activation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The data were statistically analyzed with Prism 8 (GraphPad Software, La Jolla CA, USA). All data were expressed as mean ± standard deviation (SD). The sample sizes used in each experiment were based on our previous studies16 (link),17 (link) and recent literature.24
A power analysis was performed to detect significant differences with minimal animal numbers based on our experimental experience with 80% power. Therefore, n = 5–10 was chosen. All data were included in the analyses. The data from the behavioral test were analyzed with two-way repeated-measures analysis of variance (RM ANOVA) followed by Tukey’s or Bonferroni’s post hoc test. Western blot data was compared using two-tailed t tests. A value P < 0.05 was considered significant. Image-Pro Plus analysis software (Media Cybernetics, Silver Spring, MD, USA) was used to measure the density of specific bands from western blotting. The results were shown by the ratio of phospho-µ-opioid receptor to β-actin immunoreactivity.
A power analysis was performed to detect significant differences with minimal animal numbers based on our experimental experience with 80% power. Therefore, n = 5–10 was chosen. All data were included in the analyses. The data from the behavioral test were analyzed with two-way repeated-measures analysis of variance (RM ANOVA) followed by Tukey’s or Bonferroni’s post hoc test. Western blot data was compared using two-tailed t tests. A value P < 0.05 was considered significant. Image-Pro Plus analysis software (Media Cybernetics, Silver Spring, MD, USA) was used to measure the density of specific bands from western blotting. The results were shown by the ratio of phospho-µ-opioid receptor to β-actin immunoreactivity.
7
Wound Healing Assay for SKOV-3 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After the SKOV-3 cells were transfected for 24 h, a straight gap was created by a 200 μl sterile tip in the middle of the well. The cells were washed by DMEM 2 times for smoothing the edges of the scratch and removing floating cells. After being incubated in an incubator (37°C, 5% CO2) for 0 h and 24 h, the migration images of the cells were observed under a microscope (Keyence, Osaka, Japan). The distance of cell migration was visualized and images were taken using image-Pro Plus Analysis software (Media Cybernetics Company, USA).
8
Wound Healing Assay for VSMCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After the VSMCs had been transfected for 48 h, a straight gap was created using a 200 µl sterile tip in the middle of the well. The cells were washed twice with DMEM to smooth the edges of the scratch and remove floating cells. After incubation at 37°C for 0 or 48 h, the cells were observed under a florescent microscope (Keyence Corporation; magnification, ×100) and the distance of migration was determined. Images were captured using Image-Pro Plus Analysis software (version 6.0; Media Cybernetics, Inc.).
9
In Vitro Cell Migration Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
At 24 h post-transfection, cells in good condition were inoculated into a 6-well culture plate at a final density of 1 × 106 cells/well. When the confluence reached 95%, a 20 μL micropipette tip was used to create a vertical linear scratch in the monolayer on the 6-well plate. After removing the detached cells with a D-Hank's solution, cells were continuously cultured with serum-free medium for photographing. The cell migration distance was measured using Image-Pro Plus Analysis software (Media Cybernetics Company, MD, USA) at 0 and 48 hours after scratching under a light microscope.
10
Evaluating Cell Migration and Invasion
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
5637 and HT1376 cells were transfected with pEGFP-N1 or pEGFP-FBLN1 for 72 h. About 1 × 105 of 5637 cells or 6 × 104 HT1376 cells were plated in the upper chambers of 24-well Transwell plates (Corning) in FBS-free medium. Complete medium (10% FBS) was deposited in the lower chambers to serve as a chemo-attractant. After 12 h for 5637 or 10 h for HT1376, cells remaining on the upper filter were removed, while cells that passed through the Transwell filter were stained by 0.5% crystal violet solution for 15 min. Images were taken of six random optical fields (200×) on each filter and cell number was quantified by utilizing the Image-Pro Plus analysis software (Media Cybernetics). To evaluate cell invasion, Transwell membranes were coated with Matrigel (BD Biosciences) prior to plating infected cells. The Matrigel served as a basement membrane barrier that cells would have to destroy in order to invade the lower chamber. After 22 h for 5637 or 18 h for HT1376, crystal violet staining and cell counting were performed as above.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!