N 2 hexahydro 2 oxo 1h azepin 3 yl amino carbonyl phenyl benzo b thiophene 2 carboxamide ana 12

Fermented Perilla frutescens (FPF), uracil, adenine, PCA, L7dGn, A7dGn and L7Gn were provided by Huons Co Ltd. (Seoul, Republic of Korea). Corticosterone, FXT and N-[2-[(hexahydro-2-oxo-1H-azepin-3-yl)amino]carbonyl]phenyl-benzo[b]thiophene-2-carboxamide (ANA-12) were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Corticosterone enzyme-linked immunosorbent assay (ELISA) kit and dopamine ELISA kit were purchased from Enzo Life Sciences (Farmingdale, NY, USA). Serotonin ELISA kit and ACTH ELISA kit were purchased from Abcam (Cambridge, UK). Anti-BDNF, anti-CREB, anti-pCREB, anti-ERK, anti-pERK, anti-TrkB, and anti-GAPDH antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-pTrkB antibody was purchased from Abcam (Cambridge, MA, USA). All other chemical reagents were purchased from Sigma-Aldrich and were of analytical or HPLC grade.

L7Gn was provided by Huons Co. Ltd. (Ansan, Korea). Fluoxetine (FXT) and N-[2-[(hexahydro-2-oxo-1H-azepin-3-yl)amino]carbonyl]phenyl-benzo[b]thiophene-2-carboxamide (ANA-12) were purchased from Sigma-Aldrich (Saint Louis, MO, USA). The plasma corticosterone ELISA kit was purchased from Enzo Life Sciences (Farmingdale, NY, USA). Anti-BDNF (#47808), anti-cAMP response element-binding protein (CREB) (#9104), anti-p-CREB (#9196), anti-extracellular signal-regulated kinase (ERK) (#9102), anti-p-ERK (#9101), anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (#2118), and anti-TrkB (#4603) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-p-TrkB (ab229908) antibody was purchased from Abcam (Cambridge, MA, USA).