Bc 08

Weight was measured with light clothing and without shoes using a digital scale (808Seca, Germany) to the nearest of 0.1 kg and the height was estimated while standing and keeping the shoulders and hips against the wall without shoes, using a stadiometer (Seca, Germany) with an accuracy of 0.1 cm. Body mass index (BMI) was calculated as weight divided by squared height and presented as kg/m². Waist circumference (WC) was measured between the lower rib and iliac crest, using a tape meter, according to standard guidelines. Waist to hip ratio (WHR) was calculated as waist circumference (cm) divided by hip circumference (cm).
After enough rest (at least 10–15 min), blood pressure was obtained by a digital barometer (BC 08, Beurer, Germany) in sitting position, and the mean of two measurements reported for each person.

We used a stadiometer with a sensitivity of 0.1 cm (Seca, Hamburg, Germany) and a digital scale instrument with a precision of 0.1 kg (808Seca; Seca) to evaluate anthropometric measures compromising body height and weight. Participants were dressed in light clothing without shoes. Body Mass Index (BMI) was calculated individually and participants were divided into four categories of underweight (< 18.5), normal (≤ 18.5–24.9≥), overweight (≤ 25-29.9≥), and obese (≥ 30) (30 ). Waist and hip circumferences were measured between the iliac crest and lower ribs by a flexible measuring tape. Physical activity was evaluated using a validated International Physical Activity Questionnaire (IPAQ) (31 (link)). Systolic and diastolic blood pressure were evaluated in a sitting stance by a digital sphygmomanometer (BC 08; Beurer, Ulm, Germany) after a resting time of ten to fifteen minutes. Blood pressure was measured twice in each participant and the average amount was entered into the analysis.

Anthropometric variables consisting of weight, height, and waist circumference (WC) were measured. Height was measured using a wall stadiometer (Seca, Germany) and recorded to the nearest 0.1 cm. Weight was measured by digital scales for adults (808Seca, Germany with a sensitivity of 0.1 kg). WC was measured by flexible anthropometric tape midway between the iliac crest and lower rib margin with a sensitivity of 0.1 cm. Anthropometric measurements were performed without shoes and heavy clothing (coat and jacket). Body mass index (BMI) was calculated as weight in kilograms divided by the square of height in meters. Systolic (SBP) and diastolic (DBP) blood pressure were measured twice by a trained physician, by the use of a digital barometer (BC 08, Beurer, Germany) after at least 10–15 min of setting. The second measurement was performed 1–2 min later and then averaged over the two measurements. Fasting plasma glucose (FPG), serum triglyceride (TG), and high-density lipoprotein (HDL) cholesterol were measured after 10–12 h of overnight fasting from a venous blood sample. Blood samples were measured by standard methods at the Nutrition and Biochemistry Laboratory of the School of Nutritional Sciences and Dietetics at Tehran University of Medical Sciences.

Information about sex (male/ female), age (year), educational level (under diploma/ diploma and higher), smoking status (never or former smoker/ current smoker), occupation (employed/ house keeper/ retired/ unemployed) and marital status (single/ married/ divorced) were collected via demographic questionnaire during the initial visit.
Physical activity was assessed using the short form of the International physical activity questionnaire (IPAQ) [30 (link)], consisting of 7 validated questions. Data was collected regarding walking, moderate, and vigorous activity, in the previous week, and a physical activity level was determined within two categories of metabolic equivalents (METs) [31 (link)], as low (< 600 MET-minutes/week) or moderate and high physical activity (> 600 MET-minutes per week).
Systolic (SBP) and diastolic blood pressure (DBP) were measured twice by a standard mercury sphygmomanometer (BC 08; Beurer, Ulm, Germany), on the right arm, after 15 min of resting, performed by a trained physician. The second measurement was done 1–2 min later. The mean of the two measurements was reported as the blood pressure of each individual.

Participants' blood pressure was measured by a digital sphygmomanometer (BC 08, Beurer, Germany) after at least 10–15 min of rest and sitting. Blood pressure was measured twice per person and the average blood pressure per person was reported to report systolic and diastolic blood pressure (SBP and DBP), respectively. The height of patients was estimated by a wall gauge (stadiometer) with a sensitivity of 0.1 cm (Seca, Germany) without shoes, and weight was obtained with a digital scale (808 Seca, Germany) with an accuracy of 0.1 kg with light clothing (without coat and raincoat). Body mass index (BMI) was calculated by dividing weight in kilograms by height squared in meters. Waist circumference (WC) was measured with a tape measure between the lowest rib and the iliac crest when exhaling.

Patient height was measured using a stadiometer with a sensitivity of 0.1 cm (Seca, Germany) under the condition that the participants were unshod, and their weight was measured by digital scales (808Seca, Germany), measured with 0.1 kg precision with light clothing (without coat and jacket). Body mass index (BMI) was calculated as weight in kg divided by squared height in meters squared. Waist circumference (WC) was recorded to the nearest 0.1 cm at the umbilical level, and hip circumference at the maximal level over light clothing, using a non-stretch tape measure, without putting pressure on the body surface. Blood pressure was measured by a digital barometer (BC 08, Beurer, Germany) after at least 10-15 minutes of rest and sitting, with the mean of two measurements being reported for each person.

People's blood pressure by digital manometer (BC 08, Beurer, Germany) after 10–15 min’ rest was measured while sitting. Blood pressure was measured twice for each person and the average blood pressure was reported for each person. The waist circumference, between the lowest gear and the iliac crest in the exhalation mode, was measured with a tape measure. Of all participants, 5 ml of blood was taken in fasting between 7–10 A.M and was poured into acid-washed test tubes without anticoagulation until after keeping at room temperature for 30 min and after blood clotting would centrifuge at 1500 g for 20 min. The serums were poured into clean microtubules and stored in a freezer at -80 C ° until the experiment. Measurement of serum glucose and lipid profile using enzymatic method, based on colourimetry, using commercial kits (Pars Azmoun, Iran) with the automatic device (Selecta E, Vitalab, Netherland) done. The experiments were performed on the day of sampling.