Dulbecco s mem medium

Manufactured by Harvard Bioscience

Sourced in Germany

Dulbecco's MEM medium is a widely used cell culture medium developed by Dr. Harry Eagle. It provides a balanced salt solution and essential nutrients to support the growth and maintenance of a variety of cell types in vitro.

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Lab products found in correlation

Penicillin, by Harvard Bioscience (3 mentions) Streptomycin, by Harvard Bioscience (3 mentions) Fbs superior, by Harvard Bioscience (3 mentions) Trypsin edta solution, by Harvard Bioscience (3 mentions) L glutamine, by Harvard Bioscience (3 mentions) D luciferin beetle monosodium salt, by Promega (2 mentions) Sodium chloride (nacl), by Merck Group (2 mentions) Viafect transfection reagent, by Promega (1 mentions) Pluronic pe 10500, by BASF (1 mentions) R carvone, by Merck Group (1 mentions) Ethanol, by Merck Group (1 mentions) Odorants, by Merck Group (1 mentions) Dynasore, by Bio-Techne (1 mentions) Halotag alexa fluor 488 ligand, by Promega (1 mentions) Mem non essential amino acid solution 100x, by Merck Group (1 mentions) Paraformaldehyde pfa, by Polysciences (1 mentions) Mowiol 4 88, by Carl Roth (1 mentions) Hoechst 33342, by Thermo Fisher Scientific (1 mentions)

3 protocols using dulbecco s mem medium

Isomeric Purity and Aldehyde Screening

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Isomeric purity of (Z)-4-undecenal (Z4-11Al) was 98.6%, according to gas chromatography coupled to mass spectrometry (6890 GC and 5975 MS, Agilent Technologies, Santa Clara, CA, USA). Isomeric purity of (Z)-4-nonenal (Z4-9Al) and (Z)-6-undecenal (Z6-11Al) were 97.4 and 96%, repectively. Chemical purity of these synthetic aldehydes was >99.9%. Ethanol (redistilled; Merck, Darmstadt, Germany) was used as solvent.
For the OR screening assays, the following chemicals were used: Dulbecco’s MEM medium (#F0435), FBS superior (#S0615), L-glutamine (#K0282), penicillin (10000 U/ml)/streptomycin (10000 μg/mL) (#A2212), trypsin/EDTA solution (#L2143) (Biochrom, Berlin, Germany), CaCl2∗2H2O (#22322.295), D-glucose (#101174Y), dimethyl sulfoxide (DMSO) (#83673.230), HEPES (#441476L), potassium chloride (#26764.230), and sodium hydroxide (#28244.295) (VWR Chemicals BDH Prolabo, Leuven, Belgium), sodium chloride (#1064041000, Merck, Darmstadt, Germany), ViaFect™ Transfection Reagent (#E4981, Promega, Walldorf, Germany), D-luciferin (beetle) monosodium salt (#E464X, Promega, Walldorf, Germany), Pluronic® PE 10500 (#500053867, BASF, Ludwigshafen, Germany), (R)-(−)-carvone (#W224908, Sigma-Aldrich, Steinheim, Germany).

Frey T., Kwadha C.A., Haag F., Pelletier J., Wallin E.A., Holgersson E., Hedenström E., Bohman B., Bengtsson M., Becher P.G., Krautwurst D, & Witzgall P. (2022). The human odorant receptor OR10A6 is tuned to the pheromone of the commensal fruit fly Drosophila melanogaster. iScience, 25(11), 105269.

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Odorant Screening in Cellular Assay

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The following chemicals were used: Dulbecco’s MEM medium (#F0435), FBS superior (#S0615), L-glutamine (#K0282), penicillin (100U/ml)/streptomycin (100U/ml) (#A2212), trypsin/EDTA solution (#L2143) (Biochrom, Berlin, Germany), MEM non-essential amino acid solution (100x) (#M7145, Sigma-Aldrich, Steinheim, Germany), Gibco^® HAT supplement (#21060-017, Thermo Fisher, Dreieich, Germany),CaCl₂*2H₂O (#22322.295), D-glucose (#101174Y), dimethyl sulfoxide (DMSO) (#83673.230), HEPES (#441476L), potassium chloride (#26764.230), and sodium hydroxide (#28244.295) (VWR Chemicals BDH Prolabo, Leuven, Belgium), sodium chloride (#1064041000, Merck, Darmstadt, Germany), D-luciferin (beetle) monosodium salt (#E464X), HaloTag^® Alexa Fluor^® 488 Ligand (#G1001, Promega, Madison, USA), Dynasore (#2897, Tocris Bioscience, Bristol, UK), Hoechst33342 (#1399, Invitrogen, Eugene, USA), Mowiol 4-88 (#0713, Carl Roth GmbH + Co. KG, Karlsruhe, Germany), Paraformaldehyde (PFA) (#18814, Polysciences Inc., Warrington, USA).
Odorants were purchased from Sigma-Aldrich (Steinheim, Germany), Alfa-Aesar (Karlsruhe, Germany) and Chemos GmbH (Regenstauf, Germany) (Table S1).

Noe F., Frey T., Fiedler J., Geithe C., Nowak B, & Krautwurst D. (2017). IL-6–HaloTag® enables live-cell plasma membrane staining, flow cytometry, functional expression, and de-orphaning of recombinant odorant receptors. Journal of Biological Methods, 4(4), e81.

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Odorant Receptor Activation Assay

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The following chemicals were used: Dulbecco′s
MEM medium (#F0435), FBS superior (#S0615), l-glutamine (#K0282),
penicillin (10 000U/mL)/streptomycin (10 000U/mL) (#A2212), trypsin/EDTA
solution (#L2143) (Biochrom, Berlin, Germany), calcium chloride dihydrate
(#22322.295), d-glucose (#101174Y), DMSO (#83673.230), HEPES
(#441476L), potassium chloride (#26764.230), and sodium hydroxide
(#28244.295) (VWR Chemicals BDH Prolabo, Leuven, Belgium), sodium
chloride (#1064041000, Merck, Darmstadt, Germany), D-luciferin (beetle)
monosodium salt (#E464X, Promega, Madison, USA). The following odorants
were used: 1,1,6-trimethyl-1,2-dihydronaphthalene (TDN; CAS: 30364–38–6)
(#ENAH97EDDC8E), 2-acetylnaphthalene (CAS: 93–08–3)
(#134775), and methyl isoeugenol (CAS: 93–16–3) (#W247618,
Sigma-Aldrich, Darmstadt, Germany). The KFOs used were as previously
published^{39 (link),40 (link)} (Table S1). Further
details including the GC–O approach for the purity testing
of the odorants prior to their use in the odorant receptor experiments
are available in the literature.^{41 (link)}

Haag F., Frey T., Ball L., Hoffmann S, & Krautwurst D. (2024). Petrol Note in Riesling – 1,1,6-Trimethyl-1,2-dihydronaphthalene (TDN) Selectively Activates Human Odorant Receptor OR8H1. Journal of Agricultural and Food Chemistry, 72(9), 4888-4896.

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