T24T cells transfected with the plasmids were harvested and stained with propidium iodide buffer (BD PharMingen) for cell-cycle analysis. The results were analyzed by the ModFit LT software.
Propidium iodide buffer
Manufactured by BD
Sourced in United States
Propidium iodide buffer is a solution containing propidium iodide, a fluorescent dye that binds to DNA. It is commonly used in cell biology and flow cytometry applications to stain and identify cell nuclei.
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Lab products found in correlation
8 protocols using propidium iodide buffer
1
Cell Cycle Analysis of T24T Cells
2
Cell Cycle Analysis of Transfected T Cells
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EJ and T24 T cells transfected with the plasmids or treated with MJ were harvested and stained with propidium iodide buffer (BD Pharmingen) for cell cycle analysis. The results were analyzed by the ModFit LT software.
3
Cell Cycle and Apoptosis Analysis
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ESCC cells were collected and digested at 48 hours after transfection. For the cell cycle analysis, the cells were fixed in 70% ethanol precooled at −20°C. On the second day, the cells were stained with propidium iodide buffer (BD Biosciences, San Jose, CA, USA) in flow cytometry (FCM) tubes to detect the cell cycle distribution. Annexin-V and 7-AAD dyes were prepared for the cell apoptosis assay. The cells were incubated in the dark for about 15 minutes. Cell apoptosis and the cell cycle distribution were examined by using a FACSCalibur flow cytometer (BD Biosciences, San Jose, CA, USA). The data were analyzed by FlowJo software.
4
Cell Cycle Analysis of Transfected Cells
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According to the product manual, the transfected plasmid-treated cells were collected and stained with propidium iodide buffer (BD, USA) for cell cycle analysis. The results were analyzed by the ModFit LT software.
5
Cell Cycle Analysis by Flow Cytometry
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EJ and UMUC3 cells stably transfected with plasmids were harvested to analyze cell cycle by flow cytometry (Becton Dickinson, NJ, USA) after stained with propidium iodide buffer (BD Pharmingen). The results were analyzed with ModFit LT software.
6
Cell Cycle Analysis of Transfected HCC Cells
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HCC Cells were transfected with siRNA or plasmid for 48 h. Approximately, one million cells were harvested and fixed with 75% ethanol at 20°C overnight. The cells were stained with propidium iodide buffer (BD, NJ) at 37 °C for 30min. The cell cycle was analyzed by a FACSCalibur flow cytometer (Becton Dickinson).
7
Bladder Cancer Cell Cycle and Apoptosis
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For the cell cycle assay, bladder cancer cells were seeded into a six-well plate. Cells were harvested to analyze the proportion of cell apoptosis by flow cytometry (Becton Dickinson) after stained with propidium iodide buffer (BD PharMingen). The results were analyzed by the ModFit LT software.
For the apoptosis assay, bladder cancer cells were harvested to analyze cell apoptosis by flow cytometry (Becton Dickinson) after stained with FITC Annexin V and propidium iodide (PI) staining (BD Pharmingen). FlowJo software was used to analyze the results.
For the apoptosis assay, bladder cancer cells were harvested to analyze cell apoptosis by flow cytometry (Becton Dickinson) after stained with FITC Annexin V and propidium iodide (PI) staining (BD Pharmingen). FlowJo software was used to analyze the results.
8
Clonogenic Assay and Cell Migration
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Bladder cancer cells were seeded in the 6-well plate with 1000 cells/well. Subsequently, the plates were placed in the incubator with standard culture. Finally, clones were harvested and stained with 0.1% crystal violet (Beyotime, Shanghai, China) when over 50 cells per clone were counted. The migration and matrigel invasion assays were performed to evaluate cell migration and invasion abilities by using transwell chamber as described previously. 13 Flow cytometry assay (FCM) for cell apoptosis and cell cycle analyses Cell apoptosis were tested by staining cells with Annexin V-uorescein isothiocyanate (FITC) and propidium iodide (PI) (BD Biosciences, USA) according to the manufacturer's instructions. Flowjo VX10 software was used for analyzing the results. For cell cycle analyses, cells were harvested and stained with propidium iodide buffer (BD Biosciences, USA) according to the manufacturer's instructions. The ModFit LT software was used for analyzing the results.
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