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16 protocols using dragon skin 10

1

Oxygen Sensing System Characterization

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The responsiveness of the oxygen sensing system to changes in media oxygen concentration and flow rate was characterized using 96-well plates and a new oxygen-impermeant version of the HT-µUPS cover described in our previous work (Wei et al., 2020 (link)). This cover was assembled in two components, one soft PDMS sealing gasket and one acrylic perfusion base that was CNC-milled from cast acrylic (McMaster-Carr 8560K354) and thermally bonded using a heat press (Rosineer Grip Twist) at 130°C for 3 h. The sealing gasket was fabricated by pouring a mixture of Sylgard 184 (Dow) and Dragonskin 10 (Smooth-On) in a 1:2 ratio into an acrylic mold. Finally, an inlet and outlet were tapped (10–32 thread) for each well and fitted with stainless steel 10–32 barb-to-thread connectors (Pneumadyne).
The sensor response within the perfusion system HT-µUPS was validated using the setup in Figure 3A. Clark electrodes were placed before and after the cover. An optical oxygen sensor was placed at the bottom of the leftmost well of the cover. The Clark electrodes and VisiSensTD system were calibrated with water at 100% O2 and 0% O2. Water bubbled with 100% O2 was loaded into a syringe pump and perfused through the cover. Flow rate was measured using a flow meter (Sensirion SLI-2000) and varied between 500 ml/min and 200 ml/min to determine the system’s response to changes in flow.
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2

Fabrication of Soft Actuator Prototypes

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Two methods and four materials were used to fabricate the soft actuators. Two methods are: (1) the traditional casting process, (2) 3D printing using the Objet350 Connex3 printer (Stratasys, Minnesota, USA) and the Agilista printer (Keyence, Japan). Four materials are: (1) the Dragon Skin 10 (Smooth-on Inc., PA, USA), (2) the Ecoflex (Smooth-on Inc., PA, USA), (3) the TangoPlus or TangoBlackPlus (Stratasys, MN, USA), which mainly consists of propenoic acid, ethyl ester, and trimethylbicyclo, and has a hardness of Shore A26-A28 and an elongation at break of 170–220%, and (4) the AR-G1L (Keyence, Japan), which mainly consists of silicone and acrylate monomer, and has a hardness of Shore A35 and an elongation at break of 160%.
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3

3D Assembly of Thermoelectric Precursor

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Bilayers of Ti (60 nm)/Au (60 nm) deposited by electron beam evaporation and patterned by photolithography and wet etching served as electrical interconnects between the p- and n-type silicon. This process also defined electrode pads for probing. Spin coating another layer of PI (4 μm) and patterning it by exposure to an oxygen plasma through a mask of photoresist (10 μm, AZ 4620, MicroChem) encapsulated the system and completed the fabrication of the 2D thermoelectric precursor. Dissolving the residual photoresist and the underlying PMMA in acetone allowed the precursor to be retrieved onto a piece of water-soluble tape (Aquasol). A pattern of SiOx (50 nm) formed by electron beam evaporation through a shadow mask defined bonding sides on the back side of the precursor. An elastomer substrate (Dragon Skin 10, 1:1, Smooth-On Inc.) stretched to the desired level using a stage served as a substrate for 3D assembly. Exposing the elastomer and the 2D precursor (still on a water-soluble tape) to ultraviolet-induced ozone (Jelight UVO-Cleaner, 144AX) and then laminating to the two together and baking them in a convection oven at 70°C formed strong adhesion via condensation reactions at the bonding site interface. Dissolving in warm water removed the tape. Slowly releasing the strain in the elastomer substrate while immersed in water completed the 3D assembly.
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4

Controlled-release silk hydrogel BioDomes

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The BioDomes were composed of a soft silicon insert that contained silk hydrogels as a controlled-release substrate and drug carrier. The fabrication of the device has been reported elsewhere (34 (link)). Briefly, the outer cylindrical silicon sleeve (20-mm H × 18-mm D) was fabricated by casting silicon elastomer (Dragon skin 10, Smooth-on, Macungie, PA) against a 3D-printed mold, which was designed using CAD software (SolidWorks, Waltham, MA, USA) and printed using a Formlabs 3D printer (Somerville, MA, USA).
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5

Elastomeric Metamaterial Fabrication

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In this work, experiments are conducted on building blocks of 2 × 2 elastomeric rotating squares and larger 10 × 10 metamaterials (Fig. 1c). The squares have edge length d = 12 mm and are rotated by an angle θLin = 5° with respect to the vertical axis (note that θLin is the initial equilibrium angle without magnets inserted). We print a mold (MakerGear M2, Polylactic acid (PLA)) with cylindrical extrusions of radius r = 6 mm at the center (see Supplementary Fig. 1). Adjacent squares are connected via thin hinges of thickness h = 1.5 mm. Silicone (Dragonskin 10, Smooth-On, Inc.) is mixed under vacuum using a Speedmixer (FlackTek, Inc), then poured into the mold and cured at room temperature (six hours). After curing, permanent cylindrical magnets (D41-N52 Neodymium Magnets, K&J Magnetics) are embedded at the center to provide attraction between adjacent squares. Note that magnets are not included in the squares along the edges, to prevent unintended phase changes at the boundary squares that can result from boundary effects. Finally, 3D-printed (MakerGear M2, PLA), diamond-shaped trackers are adhered to the surface of each unit to allow tracking of the nodal rotation during dynamic testing.
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6

Elastomeric Matrix Fabrication for Soft Actuators

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To create an elastomer matrix for the lamina, elastomeric platinum-cure resin (Ecoflex 00–30, Smooth-On) was mixed in a 1:1 ratio by weight. The mixture was subjected to 2000 rpm for 1 min in a planetary mixer (Thinky ARE-310) and degassed at 2200 rpm for an additional minute. The pneumatic cylinders, planar actuators, and membrane actuators used in the demonstrations were also fabricated from this resin, to create soft base bladders sufficiently compatible for the STUAD-prepreg to remain adhered. Polyester continuous fibers (100% Spun Thread, PRC) were used as received. The resin for the self-adhesive backing of the lamina was created by mixing a 1:1 weight ratio of silicone adhesive constituent (Silbione 4717, Elkem Silicones). Tabs for uniaxial tension tests were made from muslin fabric (Product #: 8808K11, McMaster Carr) infused with a platinum-cure resin (Dragon Skin 10, SmoothOn).
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7

Silicone Casting Using 3D-Printed Molds

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As shown in fig. S44, the uncured silicone precursor (Dragonskin 10, Smooth-On, Inc.) is poured into the 3D-printed mold. Then, we ensure all excess silicone is removed. After 24 hours, the cured specimens are taken out and prepared for further use.
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8

Fabrication of Mechanical Bistable Valve

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The mechanical bistable valve receives a constant pressure as input and outputs a periodic pressure variation due to an internal instability, as reported previously (43 (link)). We fabricate the bistable valve based on (42 (link)). We pour the silicone precursor (Dragonskin 10, Smooth-On Inc.) into a 3D-printed mold and cure. Air tubes are glued to the membrane in the middle of the valve using Sil-poxy (Smooth-On Inc.). The cap of the valve is subsequently glued to the main body of the bistable valve. Last, we connect the air pathway.
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9

Fabrication of Elastomeric Substrate

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The cardboard (fabricated by Sichuan Guangan Hangrui Paper Products Co. Ltd.) was firstly cut into small squares. The Dragon Skin-10 (SMOOTH-ON) which is a high-performance platinum-cured liquid silicone compound, was used as the elastomer substrate. Part A and B of the Dragon Skin were mixed in a ratio of 1 : 1 and were poured onto the cardboard surface. The mixture was cured at 60 °C for 30 min in a vacuum drying oven (Shanghai Yuejin Medical Instruments).
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10

Fabrication of Silicone and Epoxy Inks

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Sylgard 184 and Sylgard 186 are purchased from Dow Corning, USA. DragonSkin 30, DragonSkin 10, Ecoflex 00-30, and Ecoflex 00-10 are purchased from Smooth-on, USA. The silicone ink was prepared by directly blending Part A and B at a specific mass ratio in a 50 ml beaker. For Sylgard 184, Part A: B = 10:1 is used unless other mixing ratios are specified. For Slygard 186, Part A:B = 10:1. For DragonSkin 30, DragonSkin 10, Ecoflex 00-30, and Ecoflex 00-10, Part A:B = 1:1. For DragonSkin 10 + 20 wt% SiO2 and blended using a planetary mixer (ARV-310, Thinky, Japan) at 2000 rpm for 5 min. The epoxy ink is purchased from Sinoepc China, and mixing E39D and E20 with 1:1 weight ratio by manual stirring under heating with a heat gun, then added 14.2 phmr 2-ethyl-4-methylimidazole (Aladdin, China) as curing agent. For all these materials, colorants are added for identification.
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