Bifidobacterium adolescentis

The transplantation experiment was carried out according to previous reports with modifications (27 (link), 28 (link)). Mice at 3 weeks of age were treated with a combination of metronidazole (1 g/L), neomycin sulfate (1 g/L), ampicillin (1 g/L), and vancomycin (0.5 g/L) (Sangon Biotech) for 2 weeks. Then, at 5 weeks of age, the water was replaced by antibiotic-free water and the mice were administered (i.g.) with a mixture of 2 × 10⁸ CFU Bifidobacterium longum (ATCC15697) and 2 × 10⁸ CFU Bifidobacterium adolescentis (ATCC15703). Also, the antibiotic-free drinking water was supplied with B. longum (final concentration ≈10⁶ CFU/mL) and B. adolescentis (final concentration ≈10⁶ CFU/mL). Mice in the control group were administered (i.g.) with an equal volume of Modified Reinforced Clostridial Medium (BD 218081) but without bacterial cells and the antibiotic-free drinking water was also supplied with an equal volume of Modified Reinforced Clostridial Medium. Mice were evaluated after 15 days.

A mixture of bacterial DNA (10 Strain Even Mix Genomic Material, MSA-1000) was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA), comprising genomic DNA prepared from the following ten bacterial strains: Bacillus cereus (ATCC 10987), Bifidobacterium adolescentis (ATCC 15703), Clostridium beijerinckii (ATCC 35702), Deinococcus radiodurans (ATCC BAA­816), Enterococcus faecalis (ATCC 47077), Escherichia coli (ATCC 700926), Lactobacillus gasseri (ATCC 33323), Rhodobacter sphaeroides (ATCC 17029), Staphylococcus epidermidis (ATCC 12228), and Streptococcus mutans (ATCC 700610).

MSA-2002 was purchased from ATCC, Manassas, VA. The sample contains a mixture of 20 different bacterial strains distributed equally (5% ea.): Acinetobacter baumannii (ATCC 17978), Actinomyces odontolyticus (ATCC 17982), Bacillus cereus (ATCC 10987), Bacteroides vulgatus (ATCC 8482), Bifidobacterium adolescentis (ATCC 15703), Clostridium beijerinckii (ATCC 35702), Cutibacterium acnes (ATCC 11828), Deinococcus radiodurans (ATCC BAA-816), Enterococcus faecalis (ATCC 47077), Escherichia coli (ATCC 700926), Helicobacter pylori (ATCC 700392), Lactobacillus gasseri (ATCC 33323), Neisseria meningitidis (ATCC BAA-335), Porphyromonas gingivalis (ATCC 33277), Pseudomonas aeruginosa (ATCC 9027), Rhodobacter sphaeroides (ATCC 17029), Staphylococcus aureus (ATCC BAA-1556), Staphylococcus epidermidis (ATCC 12228), Streptococcus agalactiae (ATCC BAA-611), and Streptococcus mutans (ATCC 700610).