6-hydroxyl-2,5,7,8-tetra methylchroman-2-carboxylic acid (Trolox), ABTS, dehydrated quercetin and gallic acid were purchased from Sigma–Aldrich Chemical Co (St Louis, MO, USA); ethanol and potassium per-sulphate were purchased from LabSynth (Diadema, SP, Brazil); Rosmarinus officinalis leaves (Santos Flora, São Paulo, Brazil), stearic acid (Viafarma, São Paulo, Brazil), oleic acid (LabSynth, São Paulo, Brazil), Poloxamer 188 (kindly donated by BASF, Brazil), Arabic gum (NEXIRA, Brazil), whey protein (Arla Foods Ingredients S.A., Argentina), ethanol (LabSynth, Diadema, SP, Brazil), and ultrapure water was obtained by Milli Q filtration system (Millipore, Germany).
Ethanol
Manufactured by Labsynth
Sourced in Brazil
Ethanol is a clear, colorless liquid with a characteristic odor. It is a widely used solvent in various laboratory applications. Ethanol's core function is to dissolve and extract specific compounds from samples or mixtures, facilitating analysis and experimentation.
Automatically generated - may contain errors
Lab products found in correlation
Hydrochloric acid (hcl), by Labsynth (2 mentions)
Hplc grade acetonitrile, by Avantor (2 mentions)
Methanol, by Merck Group (2 mentions)
Poloxamer 188 pluronic f 68, by Merck Group (1 mentions)
Dichloromethane dcm, by Labsynth (1 mentions)
Sodium hydroxide (naoh), by Labsynth (1 mentions)
Carboxymethylcellulose, by Labsynth (1 mentions)
Nα benzoyl dl arginine p nitroanilide hydrochloride, by Merck Group (1 mentions)
Acetic acid, by Labsynth (1 mentions)
Rpmi 1640 culture medium, by Thermo Fisher Scientific (1 mentions)
Guava easycyte mini flow cytometry system, by Merck Group (1 mentions)
Dichloromethane, by Labsynth (1 mentions)
Guava cell cycle reagent, by Merck Group (1 mentions)
Crystal violet, by Merck Group (1 mentions)
Guava nexin reagent, by Merck Group (1 mentions)
Penicillin streptomycin, by Vitrocell (1 mentions)
Piroxicam, by Pfizer (1 mentions)
Acetone, by Labsynth (1 mentions)
Eugenol, by Merck Group (1 mentions)
Pluronic f68, by Merck Group (1 mentions)
12 protocols using ethanol
1
Antioxidant Potential of Rosmarinus officinalis
2
Chloroquine Diphosphate-Loaded Polymeric Nanoparticles
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Chloroquine Diphosphate (CQ), Poloxamer 407 (Pluronic® F-127), and Poloxamer 188 (Pluronic® F-68) were purchased from Sigma-Aldrich (Saint Louis, MO, USA). d , l -poly(lactic acid) (PLA) (inherent viscosity 0.67 dL g−1 at 25 °C) was purchased from LACTEL Absorbable Polymers® (Birmingham, AL, USA). Dichloromethane (DCM) (dielectric constant (ε) of 9.1), ethanol (EtOH) (ε of 24.6), acetone (ACE) (ε of 20.6) and N-Methyl-2-pyrrolidone (NMP) were purchased from Labsynth® (São Paulo, Brazil). The purified water (1.3 µS cm−1) was prepared from reverse osmosis purification equipment, model OS50 LX, Gehaka (Sao Paulo, Brazil). All other reagents have analytical grade.
3
Papain Enzyme Characterization Protocol
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Carboxymethylcellulose, poly(vinyl alcohol) (98% hydrolyzed), L-cysteine hydrochloride monohydrate, dimethylsulfoxide, ethanol, sodium hydroxide, acetic acid, ethylenediaminetetraacetic acid, monosodium phosphate, and heptahydrate disodium phosphate were purchased from LabSynth (Diadema, Brazil). Papain 30.000 USP-U/mg was acquired from Merck (Darmstadt, Germany) and Nα-benzoyl-DL-arginine-p-nitroanilide hydrochloride from Sigma-Aldrich (San Luis, MO, USA). All reagents were of analytical grade.
4
Anti-inflammatory Activity Evaluation
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All solvents and chemicals were purchased from commercial sources and were used without further purification. Dichloromethane and ethanol (analytical grade) were obtained from Labsynth (Diadema, SP, Brazil). Trichloroacetic acid, Tween 80®, dexamethasone (purity > 90%), dimethylsulfoxide (DMSO), carrageenan, croton oil and hexadecyltrimethylammonium bromide buffer (HTAB) were obtained from Sigma-Aldrich Laboratories (San Loius, MO, USA). Piroxicam was obtained from Pfizer (New York, NY, USA). Culture medium RPMI 1640 and fetal bovine serum (SBF) were from Gibco (Waltham, MA, USA). Penicillin/streptomycin (1000 U/mL:1000 mg/mL) and trypsin-EDTA 0.25% were acquired from Vitrocell (Campinas, SP, Brazil). Doxorubicin (chlorohydrate 50 mg; purity > 90%) was obtained from Eurofarma (São Paulo, SP, Brazil). Crystal violet, Guava Nexin Reagent, Guava Multicaspase kit (Caspase Reagent Working Solution, Apoptosis Wash Buffer and Caspase 7-AAD Reagent Working Solution) and Guava Cell Cycle Reagent were acquired from Merck-Millipore (Darmstadt, Germany). Flow cytometry experiments and analysis were performed using a Guava EasyCyte Mini Flow Cytometry System (Millipore). Plethysmometer apparatus (7140) was purchased from Ugo Basile (Gemonio, VA, Italy) and a grinder (Disperser T 10 basic) from IKA (Campinas, SP, Brasil).
5
Antimicrobial Efficacy of Garlic Oil Formulation
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Garlic oil (98%), eugenol
(99%), zein, and Pluronic F-68 were purchased from Sigma-Aldrich.
Acetone, ethanol, and isopropanol were purchased from Labsynth. The
solvent employed for the chromatographic analyses was HPLC-grade acetonitrile
(JT Baker, Phillipsburg, New Jersey). The bacteria A. hydrophila (ATCC-7966), E. tarda (ATCC-15947), and S. iniae (ATCC-29177)
were purchased from PASTLABOR-Brazil).
(99%), zein, and Pluronic F-68 were purchased from Sigma-Aldrich.
Acetone, ethanol, and isopropanol were purchased from Labsynth. The
solvent employed for the chromatographic analyses was HPLC-grade acetonitrile
(JT Baker, Phillipsburg, New Jersey). The bacteria A. hydrophila (ATCC-7966), E. tarda (ATCC-15947), and S. iniae (ATCC-29177)
were purchased from PASTLABOR-Brazil).
6
Optimizing HPLC Analysis of Yerba Mate
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The standards of the compounds analyzed by liquid chromatography (chlorogenic acid, caffeine, and rutin) were provided by Sigma Aldrich (St. Louis, MO, USA). HPLC-grade acetonitrile and mEthanol were supplied by the company Merck (São Paulo, Brazil). The ultra-pure water was provided by a Purelab Flex 3 purifying system (Elga Veolia, High Wycombe, United Kingdom). Ethanol (99.5%), phosphoric acid (85%), hydrochloric acid, and sodium hydroxide were purchased from Labsynth (São Paulo, Brazil). The adsorbents used in this study were: Sepra C18-E, (particle size: 50 μm, pore size: 85 Å, Phenomenex, Torrance, CA, USA); Isolute C18-EC, (particle size: 50 μm, poresize: 60 Å, Biotage, Sweden) and Strata X C18 (particle size: 33 μm, pore size: 85 Å, Phenomenex, Torrance, CA, USA).Yerba mate leaves were acquired in a local supermarket and stored at −20 °C until used as a sample. The particle size of the sample used for the experiments was between 1.41 and 2.00 mm.
7
Formulation and Characterization of Insect Repellent
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Ethyl butyl acetylamine propionate butylacetylaminopropionate 98% (IR3535) and icaridin 97% (ICA) was obtained from Chemidin (China). Geraniol 98% (GRL) was obtained from Quinarí (Ponta Grossa, Paraná, Brazil). Tripalmitin, polyvinyl alcohol (PVA), and poloxamer 407 (PL407) were obtained from Sigma-Aldrich (St. Louis, USA). Mirytol was obtained from BASF. Ethanol was purchased from Labsynth (Diadema, São Paulo, Brazil). Acetonitrile (HPLC grade) was obtained from J.T. Baker (USA). All other reagents were obtained from local suppliers in Sorocaba (São Paulo, Brazil).
8
Cardiovascular Pharmacology Protocol
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Phenylephrine hydrochloride (Sigma-Aldrich, St. Louis, MO, USA), sodium nitroprusside (Sigma-Aldrich), acetylcholine (Sigma-Aldrich) and tribromoEthanol (Sigma-Aldrich) were dissolved in saline (0.9% NaCl). Ethanol (Labsynth, Diadema, SP, Brazil) was diluted in the drinking water. Testosterone propionate (PharmaNostra, Rio de Janeiro, RJ, Brazil) was dissolved in almond oil. Flunixine meglumine (Banamine®, Schering-Plough, Cotia, SP, Brazil) and the poly-antibiotic preparation (Pentabiotico®, Fort-Dodge, Brazil) were used as provided.
9
Fabrication and Characterization of ZnO-NPs Enhanced Cotton Fabrics
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The cotton fabrics (CF) were purchased from a local fabric store (São Paulo, Brazil). ZnO-NPs (< 50 nm) were acquired from Sigma-Aldrich Inc. (São Paulo, Brazil). Anhydrous gallic acid was obtained from InLab. Phenol, neutral detergent, ethanol, l -ascorbic acid, trisodium citrate, and silver nitrate were obtained from Labsynth (São Paulo, Brazil). The water-based acrylic binder (Acrylic Resin 180 W) was purchased from Redelease (São Paulo, Brazil). Nutrient agar culture medium was purchased from Himedia. Methylene blue (MB) was acquired from Merck.
10
Evaluation of Antioxidant Compounds
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The chemicals used in this study are as follows: methanol (A1085.01.BJ), aluminum chloride (C1036.01.AG), ethanol (A1084.01.BJ), and hydrochloric acid (A1028.01.BJ; Labsynth, Diadema, SP, Brazil); sodium tungstate-2-hydrate (230), triton X-100, and phosphomolybdic acid (443; Vetec Química Fina Ltda, Duque de Caxias, RJ, Brazil); gallic acid monohydrate (27645-250G-R), 1,1-diphenyl-2-picrylhydrazyl (DPPH•; D9132), luminol (5-amino-2,3-dihydro-1,4-phthalazinedione; A8511-5G), phorbol-12-myristate-13-acetate (PMA; P8139), and Trypan blue (T6146-25G; Sigma-Aldrich, St. Louis, MO, USA); gelatin (microbiological grade; 214320, Difco, Laboratories, Detroit, MN, USA); dimethyl sulfoxide (DMSO; 3317275) (Merck-Schuchardt, Hohenbrunn, DE); “Kit” to determine lactate dehydrogenase enzyme (LDH Liquiform; 86-2/30—Labtest Diagnostica, Lagoa Santa, MG, Brazil). Drying carriers used are colloidal silicon dioxide (Aerosil 200, Evonik Degussa, Hanau, Germany), maltodextrin (MOR-REX 1910, Corn Products do Brasil), β-cyclodextrin (Kleptose; Roquette, Lestrem, FR), and gum Arabic (Encapsia; NEXIRA do Brasil, São Paulo, SP, Brazil).
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The chemicals used in this study are as follows: methanol (A1085.01.BJ), aluminum chloride (C1036.01.AG), ethanol (A1084.01.BJ), and hydrochloric acid (A1028.01.BJ; Labsynth, Diadema, SP, Brazil); sodium tungstate-2-hydrate (230), triton X-100, and phosphomolybdic acid (443; Vetec Química Fina Ltda, Duque de Caxias, RJ, Brazil); gallic acid monohydrate (27645-250G-R), 1,1-diphenyl-2-picrylhydrazyl (DPPH•; D9132), luminol (5-amino-2,3-dihydro-1,4-phthalazinedione; A8511-5G), phorbol-12-myristate-13-acetate (PMA; P8139), and Trypan blue (T6146-25G; Sigma-Aldrich, St. Louis, MO, USA); gelatin (microbiological grade; 214320, Difco, Laboratories, Detroit, MN, USA); dimethyl sulfoxide (DMSO; 3317275) (Merck-Schuchardt, Hohenbrunn, DE); “Kit” to determine lactate dehydrogenase enzyme (LDH Liquiform; 86-2/30—Labtest Diagnostica, Lagoa Santa, MG, Brazil). Drying carriers used are colloidal silicon dioxide (Aerosil 200, Evonik Degussa, Hanau, Germany), maltodextrin (MOR-REX 1910, Corn Products do Brasil), β-cyclodextrin (Kleptose; Roquette, Lestrem, FR), and gum Arabic (Encapsia; NEXIRA do Brasil, São Paulo, SP, Brazil).
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