UNBS5162, amonafide, and 5FU were purchased from the American MedChemExpress, Monmouth Junction, NJ, USA. Further, reference antibodies Bcl-2 (proteintech, Rosemont, IL, USA; Cat# 12789–1-AP), active-Caspase3 (Abcam, Cam-bridge, UK; Cat# ab32042), Bax (proteintech, Cat# 50599–2-Ig), P70/S6K (Abcam, Cat# ab109393), AKT (Abcam, Cat# ab8805), p-AKT (Abcam, Cat# ab38449), mTOR (Abcam, Cat# ab32028), p-mTOR (Abcam, Cat# ab109268), Cyclin D1 (proteintech, Cat# 12363–1-AP), E-cad (Abcam, Cat# ab40772), Snail (proteintech, Cat# 13099–1-AP), N-cad (Abcam, Cat# ab6528), Twist (proteintech, Cat# 11752–1-AP), Slug (proteintech, Cat#) and GAPDH (proteintech, Cat# 60004–1-Ig) were used in this study. Our electrochemiluminescence kit was obtained from proteintech.
Electrochemiluminescence kit
Manufactured by Proteintech
The Electrochemiluminescence (ECL) kit is a laboratory tool used for the detection and quantification of various analytes, such as proteins, peptides, and small molecules. The kit utilizes an electrochemical reaction to generate luminescence, which can be measured using specialized equipment. The core function of the ECL kit is to provide a sensitive and reliable method for analyte detection and quantification.
Automatically generated - may contain errors
Lab products found in correlation
Gapdh, by Proteintech (2 mentions)
Bcl2 associated x (bax), by Proteintech (2 mentions)
P70s6k, by Abcam (1 mentions)
E cad, by Abcam (1 mentions)
N cad, by Abcam (1 mentions)
Twist, by Proteintech (1 mentions)
P akt, by Abcam (1 mentions)
P mtor, by Abcam (1 mentions)
Active caspase 3, by Abcam (1 mentions)
Snail, by Proteintech (1 mentions)
Cyclin d1, by Proteintech (1 mentions)
Bcl 2, by Proteintech (1 mentions)
P akt, by Cell Signaling Technology (1 mentions)
Caspase 3 p17, by Proteintech (1 mentions)
Rabbit anti human akt, by Cell Signaling Technology (1 mentions)
Unbs5162, by MedChemExpress (1 mentions)
P mtor, by Cell Signaling Technology (1 mentions)
2 protocols using electrochemiluminescence kit
1
Multiomics Evaluation of Anticancer Agents
2
Signaling Pathways in UNBS5162 Treatment
Check if the same lab product or an alternative is used in the 5 most similar protocols
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UNBS5162 was obtained from MedChemExpress (Princeton, NJ, USA). All experiments were performed using 10 μM UNBS5162 as the work concentration. Reference antibodies were obtained: rabbit antihuman Akt, p-Akt, mTOR, p-mTOR (Cell Signaling Technology, Danvers, MA, USA), rabbit antihuman p70, Bcl2, Bax, caspase 3 p17, mouse antihuman cyclin D1, and GAPDH (ProteinTech Wuhan, China). The electrochemiluminescence kit was also purchased from ProteinTech.
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