Oneview is camera

Manufactured by Ametek

Sourced in United States

The OneView IS camera is a specialized laboratory imaging device designed for scientific and research applications. It captures high-quality images and videos with advanced image stabilization technology. The core function of the OneView IS camera is to provide stable and clear visual data for analysis and documentation purposes in a laboratory setting.

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Lab products found in correlation

Vitrobot mark 4, by Thermo Fisher Scientific (2 mentions) 2100plus, by JEOL (1 mentions) 2100plus microscope, by Ametek (1 mentions) Jem 2100, by JEOL (1 mentions) Grand arm, by JEOL (1 mentions) Axs d8 advance, by Bruker (1 mentions) Jem 2100 plus, by JEOL (1 mentions) Lr white resin, by Agar Scientific (1 mentions) Hpm100, by Leica (1 mentions) 2100 plus transmission electron microscope, by JEOL (1 mentions) Themis z stem, by Thermo Fisher Scientific (1 mentions)

12 protocols using oneview is camera

Characterization of Block Copolymer Micelles

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Carbon coated grids, carbon film on copper 300 mesh, were purchased from EM Resolutions. Solutions of CP-diblock conjugate in water at 1 mg mL⁻¹ were prepared by direct dissolution of the solid in filtered ultra-pure water. Ten microlitre of solution was dropcast on freshly glow-discharged carbon-coated grids placed on filter paper. Bright field TEM micrographs were obtained with a Jeol 2100Plus is operating at 200 kV, equipped with a Gatan OneView IS camera.

Rho J.Y., Cox H., Mansfield E.D., Ellacott S.H., Peltier R., Brendel J.C., Hartlieb M., Waigh T.A, & Perrier S. (2019). Dual self-assembly of supramolecular peptide nanotubes to provide stabilisation in water. Nature Communications, 10, 4708.

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TEM Imaging of Conjugate Nanoparticles

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10 μL of the conjugate aqueous solution was drop-casted on the carbon-coated grid. After 3 min, the solution on the grid was absorbed with filter paper. After 15 min, 10 μL of a 0.2% uranyl acetate solution was dropped onto the grid and absorbed after 30 s. Bright field TEM micrographs were obtained with a JEOL 2100Plus microscope operating at 200 kV, equipped with a Gatan OneView IS camera.

Song Q., Kerr A., Yang J., Hall S.C, & Perrier S. (2021). Tubular supramolecular alternating copolymers fabricated by cyclic peptide–polymer conjugates. Chemical Science, 12(26), 9096-9103.

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Transmission Electron Microscopy of sEVs

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The size and morphology of recovered sEVs were visualized by negative-stained transmission electron microscopy (TEM) using a JEOL JEM-2100 (Tokyo, Japan) microscope mounted on a Gatan (Pleasanton, CA, USA) OneView IS camera. To prepare the thin formvar/carbon-coated 400-mesh copper EM grids (01754-F; Ted Pella, Redding, CA, USA) for imaging, 6 µL diluted sEV solution was loaded onto the grid and incubated at room temperature for 4 minutes. The sEV dilution was determined by the concentration of the sample. After incubation, the excess sEV solution was wicked using chromatography paper, and the grid was stained with 10 µL filtered 4% uranyl acetate solution for 3 minutes. After the staining, the excess 4% uranyl acetate solution was removed by contacting the grid edge with filter paper and allowed to dry for 10 minutes before storing the grid in an EM grid box for future observation by TEM at 80 kV.

Leung J., Pollalis D., Nair G.K., Bailey J.K., Pennington B.O., Khan A.I., Kelly K.R., Yeh A.K., Sundaram K.S., Clegg D.O., Peng C.C., Xu L, & Lee S.Y. (2024). Isolation and Characterization of Extracellular Vesicles Through Orthogonal Approaches for the Development of Intraocular EV Therapy. Investigative Ophthalmology & Visual Science, 65(3), 6.

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Characterization of Li and Na Particles Using Electron Microscopy

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Powder X-ray diffraction patterns were obtained using a Bruker AXS D8 Advance diffractometer with a Cu Kα source (λ_{Cu Kα}= 1.54 Å).
TEM experiments were performed on JEOL JEM-2100 Plus (200 kV) and double aberration-corrected JEOL GrandArm (300 kV). To be specific, HRTEM images in Figs. 3–4, EFTEM images and EELS were obtained on JEOL GrandArm. The Gatan Oneview IS camera enabled fast in situ data acquisition. Other TEM results were obtained on JEOL JEM-2100 Plus. Cryogenic experiments were carried out with Gatan double tilt cooling holder (Model 636) which can sustain a low-temperature environment at −178 °C. In situ heating experiments were carried out with Protochips (Fusion 350) in situ heating holder. Lithium whisker contact experiments were carried out with an in situ STM tip holder (PicoFemto). AC-HRTEM images of lithium and sodium particles were collected under the negative spherical aberration (C_S) imaging (NCSI) condition. Average background subtracted filtering was carried out based on the script from D. R. G. Mitchell and method by Kilaas^{35 (link)}.

Liang C., Zhang X., Xia S., Wang Z., Wu J., Yuan B., Luo X., Liu W., Liu W, & Yu Y. (2020). Unravelling the room-temperature atomic structure and growth kinetics of lithium metal. Nature Communications, 11, 5367.

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Atomic-Resolution ETEM Characterization

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The
study was performed in a Hitachi HF-3300S ETEM operated at 300 kV.
The microscope is equipped with a cold field emission gun and an imaging
aberration corrector (CEOS BCOR). A scanning unit enables STEM imaging
(annular dark field, bright field, and a secondary electron detector)
as well as compositional microanalysis with EDS. The integrated complementary
metal–oxide–semiconductor (CMOS) camera (GATAN OneView
IS camera) allows for the investigation of dynamic processes by recording
HRTEM movies with up to 300 frames/s. An electron dose rate in the
range 1000–10 400 e/Å² s was used to
acquire TEM images and movies (see Tables S1 and S2). Acquisition parameters used for STEM-EDS elemental maps
and high-angle annular dark field (HAADF)-STEM images can be found
in Tables S3 and S4. The background pressure
next to the sample was ∼1.6·10^–4 Pa.
Furthermore, the nanoparticle heterostructure presented in Figures 1–6 was exposed to a maximum electron dose of ∼1.15·10⁸ e/Å² throughout the whole experiment. Additional
information about the ETEM in Lund is available elsewhere.^{68 (link)}

Seifner M.S., Snellman M., Makgae O.A., Kumar K., Jacobsson D., Ek M., Deppert K., Messing M.E, & Dick K.A. (2021). Interface Dynamics in Ag–Cu3P Nanoparticle Heterostructures. Journal of the American Chemical Society, 144(1), 248-258.

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TEM and LCTEM Analysis of Ferrofluid

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TEM and LCTEM of bulk and in situ synthesized FF were performed using a JEOL JEM-ARM300F GrandARM TEM (Tokyo, Japan) with a Gatan OneView-IS camera (Pleasanton, CA, USA) and K3-IS camera (Pleasanton, CA, USA) operated at 300 keV. Time lapse LCTEM data were acquired using Camtasia Studio 2018 (TechSmith Corporation, Okemos, MI, USA). For post-mortem analysis, LCTEM chips were opened carefully and washed gently with distilled water. Bottom chips were then placed on the standard single-tilt TEM holder, and images were collected in BF-TEM mode.

Gnanasekaran K., Korpanty J., Berger O., Hampu N., Halperin-Sternfeld M., Cohen-Gerassi D., Adler-Abramovich L, & Gianneschi N.C. (2021). Dipeptide Nanostructure Assembly and Dynamics via in situ Liquid-Phase Electron Microscopy. ACS nano, 15(10), 16542-16551.

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Characterization of Micelles using TEM

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Micelles were characterized by a JEOL F200 transmission electron microscope with a cold field emission source, two large area energy dispersive X-ray spectrometers, and a Gatan OneView IS camera. In a typical experiment, a drop of aqueous solution of micelles was cast onto a carbon-coated copper mesh, which was dried under vacuum for at least 2 h prior to experiments.

Du K., Liao P., Yang S., von Trentini D., Sharma K., Shi X., Murray C.B., Li D, & Dmochowski I.J. (2023). Chelate-functionalized magnetic micelles for sequestration of cisplatin. Nanoscale Advances, 5(15), 3955-3963.

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Cryo-TEM Imaging of Extracellular Matrix

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Cryogenic transmission electron microscopy (cryo-TEM) samples were prepared via vitrification robot (Vitrobot Mark IV, Thermo Fisher Scientific, Inc.). Ultrathin Carbon film on Quantifoil R 2/2 200 mesh copper TEM grids were purchased from Ted Pella, Inc. TEM grids were surface plasma treated for 15 s with negative polarity prior to vitrification procedure using a PELCO easiGlow glow discharge cleaning system. For sample vitrification, 4 μL of iECM solution was pipetted onto the TEM grid placed inside the Vitrobot chamber, which was maintained at 4°C and 95% relative humidity. The grid was blotted immediately with filter paper to remove excess solution, with a blot time of 4 s, a blot force (the dimensionless Vitrobot parameter for controlling the blotting pressure) of 1, a wait time of 1 s, and a drain time of 1 s, and subsequently plunge-frozen in liquid ethane. The vitrified samples were transferred to and stored in liquid nitrogen. Samples were imaged using a Gatan Elsa Cryo-Transfer Holder (maintained at ≤−170 °C) loaded into a JEOL ARM300F GrandARM TEM (300 kV), equipped with cold field emission gun (15 μA). Images were recorded using a 4k × 4k Gatan One-View-IS camera.

Spang M.T., Middleton R., Diaz M., Hunter J., Mesfin J., Banka A., Sullivan H., Wang R., Lazerson T.S., Bhatia S., Corbitt J., D’Elia G., Sandoval-Gomez G., Kandell R., Vratsanos M.A., Gnanasekaran K., Kato T., Igata S., Luo C., Osborn K.G., Gianneschi N., Eniola-Adefeso O., Cabrales P., Kwon E., Contijoch F., Reeves R.R., DeMaria A.N, & Christman K.L. (2022). Intravascularly infused extracellular matrix as a biomaterial for targeting and treating inflamed tissues. Nature biomedical engineering, 7(2), 94-109.

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High-Resolution Environmental TEM Imaging

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A Hitachi HF-3300S
environmental TEM operated at 300 kV was used
to perform the experiments. The microscope was equipped with a cold
field emission gun as the electron source, an imaging aberration corrector
(CEOS BCOR), and a complementary metal–oxide–semiconductor
(CMOS) camera (Gatan OneView IS camera). Electron dose rates of ∼1500
e/Å² s to 4400 e/Å² s were used to
acquire HRTEM images and movies (see Tables S1 and S2). The background pressure next to the sample was ∼9.20
× 10^–4 Pa. Detailed information about the used
environmental TEM and its capabilities are available elsewhere.^{90 (link)}

Seifner M.S., Hu T., Snellman M., Jacobsson D., Deppert K., Messing M.E, & Dick K.A. (2023). Insights into the Synthesis Mechanisms of Ag-Cu3P-GaP Multicomponent Nanoparticles. ACS Nano, 17(8), 7674-7684.

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High-Resolution Ultrastructural Imaging

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Samples were prepared by fixing plant material by high-pressure freezing using a Leica HPM100, followed by freeze substitution with 100% ethanol (Leica EM Auto Freeze substitution) and infiltration with LRWhite resin (Agar Scientific). Ultra-thin sections were prepared with a Leica EM UCT ultramicrotome (section thickness: 90 nm) and were collected on pioloform/carbon-coated nickel grids (Agar Scientific) and stained with 2.5% uranyl acetate and Reynolds lead citrate (Reynolds, 1963 (link)). Ultrathin sections were imaged using a JEOL-2100Plus transmission electron microscope (JEOL, Japan) equipped with a Gatan OneView IS camera (Gatan, USA).

Piovesana M., Wood A.K., Smith D.P., Deery M.J., Bayliss R., Carrera E., Wellner N., Kosik O., Napier J.A., Kurup S, & Matthes M.C. (2023). A point mutation in the kinase domain of CRK10 leads to xylem vessel collapse and activation of defence responses in Arabidopsis. Journal of Experimental Botany, 74(10), 3104-3121.

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