Lumat lb single tube luminometer

Manufactured by Berthold Technologies

The Lumat LB Single Tube Luminometer is a compact and versatile instrument designed for the measurement of luminescent samples. It features a single tube sample holder and is capable of performing sensitive luminescence measurements.

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Lab products found in correlation

Luciferase assay, by Promega (1 mentions) Dual luciferase reporter assay system, by Promega (1 mentions)

Spelling variants of this product

Tube luminometer (14 citations) Lumat luminometer (11 citations) Single Tube Luminometer (4 citations) Lumat LB 9501 Single Tube Luminometer (2 citations)

2 protocols using lumat lb single tube luminometer

Luciferase Reporter Assay for Gene Regulation

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pGL3-GPT2 promoter-LUC was co-transfect with an internal control Renilla luciferase expressing CMV-Renilla. Forty-eight hours after transfection, cells were collected for Luciferase assay according to the manufacturer's instructions (Promega). Luminescence was measured with a Lumat LB Single Tube Luminometer (Berthold). Relative luciferase activity was expressed as the ratio of luminescence normalized to the internal control. The luciferase reporter assay was performed in triplicate in at least three separate transfection experiments.

Cicatiello A.G., Sagliocchi S., Nappi A., Di Cicco E., Miro C., Murolo M., Stornaiuolo M, & Dentice M. (2022). Thyroid hormone regulates glutamine metabolism and anaplerotic fluxes by inducing mitochondrial glutamate aminotransferase GPT2. Cell Reports, 38(8), 110409.

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Dio2 Luciferase Assay in SCC Cells

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The reporter plasmids (Dio2-LUC-WT, Dio2-LUC-Δ1, Dio2-LUC-Δ2, Dio2-LUC-Δ3, Dio2-LUC-mut, TRE3-TK-LUC) and CMV-Renilla were co-transfected into SCC cells. LUC activities were measured 48 h after transfection with the Dual-Luciferase Reporter Assay System (Promega, Madison, Wisconsin, USA, cod. E1910). Luminescence was measured with a Lumat LB Single Tube Luminometer (Berthold). Differences in transfection efficiency were corrected relative to the level of Renilla Luciferase. Each construct was studied in triplicate in at least three separate transfection experiments.

Nappi A., Miro C., Pezone A., Tramontano A., Di Cicco E., Sagliocchi S., Cicatiello A.G., Murolo M., Torabinejad S., Abbotto E., Caiazzo G., Raia M., Stornaiuolo M., Antonini D., Fabbrocini G., Salvatore D., Avvedimento V.E, & Dentice M. (2023). Loss of p53 activates thyroid hormone via type 2 deiodinase and enhances DNA damage. Nature Communications, 14, 1244.

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