Lichenase-treated extracts of S. viridis vegetative tissues and grain samples were purified using solid phase extraction (SPE) cartridges packed with graphitized carbon (Varian Bond Elut Carbon 50 mg ml−1 columns) according to Ermawar et al. [61 (link)]. The ratio of DP3:DP4 oligosaccharides in the vegetative tissue extracts were analysed using either high-performance liquid chromatography (HPLC) [91 (link)] or HPAEC-PAD on a Dionex ICS-5000 chromatograph. The grain extracts were analysed using HPAEC-PAD following Ermawar et al. [61 (link)].
Bond elut carbon
Manufactured by Agilent Technologies
Bond Elut Carbon is a solid-phase extraction (SPE) product designed for the purification and extraction of organic compounds from various sample matrices. It utilizes carbon-based sorbent materials to selectively retain and concentrate target analytes prior to analysis. The core function of Bond Elut Carbon is to provide an efficient and reliable method for sample preparation and analyte isolation, enabling improved detection and quantification in analytical workflows.
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2 protocols using bond elut carbon
1
Analysis of Lichenase-Treated S. viridis Oligosaccharides
2
Analyzing Starch and β-Glucan in Flour Samples
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A small-scale version of the Megazyme Total Starch Assay (20 mg of samples), which is based on the amyloglucosidase⁄α-amylase method (McCleary 1994), was developed to estimate starch content in the flour samples. Analyses of (1,3;1,4)-β-glucan in wheat endosperm and bran were performed using a scaled down Mixed-Linkage β-Glucan Assay Kit (Megazyme International Ireland Ltd, Wicklow, Ireland), which is based on the industry standard method for barley (22) , with 15 mg of samples. To determine the ratio of the degree of polymerization (DP) of major oligosaccharide components of the (1,3;1,4)-β-glucan, which are expressed as a DP3:DP4 ratio, the oligosaccharides released by lichenase digestion during the Megazyme assay were subjected to solid phase extraction on Varian Bond Elut Carbon 50 mg/1ml columns, eluted with 55% acetonitrile, before they were analysed with the HPAEC-PAD (Dionex, model ICS-5000 LC).
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