Nunc lab tek 2 chambered coverglass slides

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Nunc Lab-Tek II Chambered Coverglass slides are a type of cell culture vessel designed for microscopy applications. The slides feature a removable chamber that allows for the culturing and observation of cells in a controlled environment.

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Lab products found in correlation

Spinning disk confocal microscope, by Zeiss (1 mentions) Poly l lysine (pll), by Merck Group (1 mentions) Zen imaging software, by Zeiss (1 mentions)

Close spelling variants of this product

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3 protocols using nunc lab tek 2 chambered coverglass slides

Mitophagy Imaging in MEFs

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MEFs were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin and 100 U/mL streptomycin at 37°C and 5% CO2. Glucose and acetoacetate containing media were made as previously described (Mishra et al., 2014 (link)). For mitophagy experiments, cells were plated on Nunc Lab-Tek II Chambered Coverglass slides (155409, Thermo) in DMEM-based media. After cells had adhered, they were washed with PBS and glucose- or acetoacetate-containing medium was applied, after which cells were allowed to grow for 4 days and then imaged. Because cells grow more slowly in acetoacetate medium, a four-fold excess of cells was plated relative to glucose medium so that both samples were at the same density on the day of imaging.

Rojansky R., Cha M.Y, & Chan D.C. (2016). Elimination of paternal mitochondria in mouse embryos occurs through autophagic degradation dependent on PARKIN and MUL1. eLife, 5, e17896.

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Sperm Isolation and Capacitation Protocol

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Cauda epididymides of Mff^gt and WT male littermates were dissected into 0.5 mL modified Tris-buffered medium (mTBM) pre-warmed to 37°C and 5% CO₂. mTBM was composed of 113.1 mM NaCl, 3 mM KCl, 7.5 mM CaCl₂, 5 mM sodium pyruvate, 11 mM glucose, 1 mM caffeine, and 20 mM Tris., as described before [29 (link)]. Sperm were released by mincing the tissue with a 26-gauge needle and incubated at 37°C and 5% CO₂ for 15 minutes to allow for swim out. After incubation, the tissue was removed, and the fluid was mixed gently and collected into 1 mL Eppendorf tubes. Sperm were washed twice by gentle centrifugation at 833 g for 5 minutes, resuspended in 0.5 mL mTBM, then incubated at 37°C and 5% CO₂ for 30 minutes for capacitation. Sperm were plated at 5×10⁵ sperm/mL in Nunc Lab-Tek II Chambered Coverglass slides (154852, Thermo), and videos were acquired as described above.

Varuzhanyan G., Chen H., Rojansky R., Ladinsky M.S., McCaffery J.M, & Chan D.C. (2021). Mitochondrial fission factor (Mff) is required for organization of the mitochondrial sheath in spermatids. Biochimica et biophysica acta. General subjects, 1865(5), 129845.

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Confocal Imaging of UV-Induced Disassembly

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All DIC microscopy analysis was performed using the Zeiss Spinning Disk Confocal microscope (Zeiss, Oberkochen, Germany) with 63× oil-immersion objective and four-well Nunc Lab-Tek II chambered coverglass slides (Thermo Fisher Scientific, Massachusetts, USA). For disassembly analysis of UV-treated THP1 monocytes, cells were added to chambers pre-coated with 1% poly-l-lysine (Sigma-Aldrich) and allowed to adhere before UV irradiation. Time-lapse microscopy was performed by collecting images every 2 min over 4 h at 37 °C, 5% CO₂. Imaging of PR8-infected THP1 monocytes was performed by collecting images ~24 h p.i. All imaging processing and data analysis were performed using ZEN imaging software (Zeiss).

Atkin-Smith G.K., Duan M., Zanker D.J., Loh L., Nguyen T.H., Koutsakos M., Nguyen T., Jiang X., Carrera J., Phan T.K., Liu C., Paone S., Oveissi S., Hodge A.L., Baxter A.A., Kedzierska K., Mackenzie J.M., Hulett M.D., Bilsel P., Chen W, & Poon I.K. (2020). Monocyte apoptotic bodies are vehicles for influenza A virus propagation. Communications Biology, 3, 223.

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