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Mplanfln 100

Manufactured by Olympus
Sourced in Germany

The MPlanFLN 100× is a high-magnification objective lens designed for use in Olympus microscopes. It provides a 100× magnification for detailed observation and analysis of microscopic samples.

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3 protocols using mplanfln 100

1

Differential Confocal Raman Microscopy

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The DDCGRBM system was constructed according to the schematic structure shown in Fig. 1: A single longitudinal mode laser (COHERENT Verdi G2) with a wavelength of 532 nm was selected as the excitation light source. A microscope objective with a numerical aperture of 0.9, 100× (OLYMPUS MPlanFLN 100×) is driven by a piezoelectric ceramic transducer (PZT, P-725.CD, Physik Instrumente, Germany) for axial scanning imaging. A two-dimensional translation stage (P-542.2CD, Physik Instrumente, Germany) was used to move the sample for horizontal scanning imaging. Raman scattered light was collected by a high-resolution C-T grating spectrometer (FHR1000, Horiba Jobin Yvon, France). A Notch Filter (LPD01-532RU-25, Semrock, USA) was used to separate reflected light and Raman scattered light. Brillouin scattered light was collected by a tandem multi-pass F-P interferometer (JRS Scientific Instruments TFP-1). Two 10 µm pinholes (Newport, PH-10) and a two-quadrant detector (Hamamatsu Photonics S3096-02) were used to construct the divided-aperture differential confocal system.
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2

Optical Extinction Spectroscopy of Hybrid Arrays

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The extinction spectra of the samples were measured by an inverted optical microscope system (ARMS, from Ideaoptics Instruments) at room temperature, where a tungsten halogen lamp (100 W) was used as the incident light source to excite the hybrid array at normal incidence. An Olympus objective (MPlanFLN ×100, NA = 0.9) was applied to collect the transmitted light from the sample, and then, the light was directed to a spectrometer equipped with a blazed grating (150 g/mm, blazed at 0.5 μm) and finally analyzed by a thermoelectrical CCD. A two-dimensional slit was placed in the real image plane of the light path to determine the size of the testing area, which was constantly maintained at 20 × 20 µm2 throughout our extinction measurements.
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3

Multimodal Fluorescence Microscopy

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FM (Olympus BX51) with filter cubes U-MWU2 (330–385 nm), U-MWB2 (450–500 nm), U-MWG2 (510–550 nm), and U-MWIY2 (530–580 nm) are used to illuminate the samples with ultraviolet, blue, green and yellow light. Olympus DP74 camera and objective lens MPlanFLN 5x, LMPlanFLN 10×, LMPlanFLN 20×, LMPlanFLN 50×, and MPlanFLN 100× are used for imaging. CIE1931 color profiles are collected using the Ocean Optics Flame spectrometer attached to the FM.
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