At each indicated time point in Figure 4 , 27 μL of blood sample was collected from tail vein of each mouse and mixed immediately with 3 μL of 0.2M EDTA. The blood samples were then drawn into heparin-treated microhematocrit capillary tubes (Fisher Scientific) and centrifuged at 16,000 g for 3 min at 4oC using a micro-hematocrit centrifuge (Thermo Scientific). Hematocrit counts were then measured with a Critocaps micro-hematocrit tube reader (Leica Microsystems Inc). Then plasma samples in the capillary tubes were collected and stored at −80oC. Twenty-fold diluted plasma samples were then used for measuring plasma Epo protein concentration using Mouse Erythropoietin Quantikine ELISA Kit (R&D), and ELISA samples were measured with Victor X3 multi-label plate reader (PerkinElmer). Paired sample Student’s t-tests were performed to analyze the statistical significance of the plasma Epo concentration and hematocrit count differences among different treatment groups.
Heparin treated microhematocrit capillary tubes
Manufactured by Thermo Fisher Scientific
Heparin-treated microhematocrit capillary tubes are specialized laboratory equipment used for the collection and analysis of small blood samples. These tubes are coated with heparin, an anticoagulant, to prevent the blood from clotting during the collection and measurement process. The tubes are designed with a small diameter to enable the collection of precise and accurate blood samples, which are then used for various clinical and research applications.
Automatically generated - may contain errors
2 protocols using heparin treated microhematocrit capillary tubes
1
Measuring Plasma Erythropoietin and Hematocrit
2
Measuring Plasma Erythropoietin and Hematocrit
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At each indicated time point in Figure 4 , 27 μL of blood sample was collected from tail vein of each mouse and mixed immediately with 3 μL of 0.2M EDTA. The blood samples were then drawn into heparin-treated microhematocrit capillary tubes (Fisher Scientific) and centrifuged at 16,000 g for 3 min at 4oC using a micro-hematocrit centrifuge (Thermo Scientific). Hematocrit counts were then measured with a Critocaps micro-hematocrit tube reader (Leica Microsystems Inc). Then plasma samples in the capillary tubes were collected and stored at −80oC. Twenty-fold diluted plasma samples were then used for measuring plasma Epo protein concentration using Mouse Erythropoietin Quantikine ELISA Kit (R&D), and ELISA samples were measured with Victor X3 multi-label plate reader (PerkinElmer). Paired sample Student’s t-tests were performed to analyze the statistical significance of the plasma Epo concentration and hematocrit count differences among different treatment groups.
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