General tissue culture materials

Manufactured by Avantor

General tissue culture materials are a collection of laboratory equipment and consumables used for the in vitro cultivation of cells, tissues, or organisms. These materials provide the necessary components and environment for the growth and maintenance of cultured biological samples.

Automatically generated - may contain errors

Lab products found in correlation

I bet151, by Bio-Techne (2 mentions) Gapdh mab374 antibody, by Merck Group (1 mentions) Ab26109, by Abcam (1 mentions) Ab139690, by Abcam (1 mentions) Ab50818, by Abcam (1 mentions) Ab128874, by Abcam (1 mentions) Anti rabbit igg a6667, by Merck Group (1 mentions) Aldefluor assay kit, by STEMCELL (1 mentions) Anti gapdh antibody, by Merck Group (1 mentions) Anti vimentin antibody, by Abcam (1 mentions) Cgp57380, by Santa Cruz Biotechnology (1 mentions) Zeb1 sirna, by Thermo Fisher Scientific (1 mentions) Anti e cadherin antibody, by BD (1 mentions) P mnk1, by Cell Signaling Technology (1 mentions) Control igg antibody, by Merck Group (1 mentions) Ez zyme chromatin prep kit, by Merck Group (1 mentions) Vimentin antibody, by Abcam (1 mentions) Ez chip, by Merck Group (1 mentions) Bet inhibitor jq1, by BPS Biosciences (1 mentions) Anti rna polymerase 2 antibody, by Merck Group (1 mentions)

3 protocols using general tissue culture materials

Chromatin immunoprecipitation protocol for BRD proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

General tissue culture materials were obtained from VWR International. Antibodies against BRD2 (ab139690), BRD3 (ab50818), BRD4 (ab128874) and IRF1 (ab26109) antibody for chromatin immunoprecipitation (ChIP) were obtained from Abcam. Antibodies against c-MYC (5605), human PD-L1 (E1L3N clone; 13684), IRF1 (8478) and control IgG (2729) antibody for ChIP were purchased from Cell Signaling Technology. Anti-BRD4 (A301–985A) antibody for ChIP was obtained from Bethyl Laboratories. PE-conjugated human PD-L1 (MIH1 clone; 12-5983-42) antibody was from Thermo Fisher. The GAPDH (MAB374) antibody was from Millipore, and α-tubulin (sc-8035) antibody and HSP90 (sc-7940) antibody were obtained from Santa Cruz Biotechnology. Secondary anti-mouse IgG (A4416) and anti-rabbit IgG (A6667) antibodies were purchased from Sigma. The BET inhibitors JQ1 and I-BET151 were obtained from Tocris Bioscience, the BET PROTAC ARV-825 was obtained from MedChem Express, and human and mouse IFN-γ was purchased from Thermo Fisher Scientific. The siRNAs against BRD2, BRD3, BRD4, c-MYC, and IRF1 were purchased from Thermo Fisher Scientific.

Ebine K., Kumar K., Pham T.N., Shields M.A., Collier K.A., Shang M., DeCant B.T., Urrutia R., Hwang R.F., Grimaldo S., Principe D.R., Grippo P.J., Bentrem D.J, & Munshi H.G. (2018). Interplay between interferon regulatory factor 1 and BRD4 in the regulation of PD-L1 in pancreatic stellate cells. Scientific Reports, 8, 13225.

+ Open protocol

+ Expand

Comprehensive Molecular Profiling of Cellular Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

General tissue culture materials were obtained from VWR International. Antibodies against eIF4E, tubulin, HSP90, ZEB1 and Dicer were obtained from Santa Cruz, while antibodies against p-eIF4E, MNK1, p-MNK1 and Drosha were purchased from Cell Signaling. Anti-GAPDH antibody was from Millipore, anti-E-cadherin antibody was obtained from BD Bioscience, while anti-vimentin antibody was from Abcam. Secondary antibodies were purchased from Sigma. CGP57380 was obtained from Santa Cruz. siRNAs against MNK1 and MNK2 were purchased from Dharmacon, ZEB1 siRNA was obtained from Life Technologies, while eIF4E and hnRNPA1 siRNAs were from Santa Cruz. Aldefluor assay kit was purchased from Stemcell Technologies.

Kumar K., Chow C.R., Ebine K., Arslan A.D., Kwok B., Bentrem D.J., Eckerdt F.D., Platanias L.C, & Munshi H.G. (2015). Differential Regulation of ZEB1 and EMT by MAPK-interacting protein kinases (MNKs) and eIF4E in Pancreatic Cancer. Molecular cancer research : MCR, 14(2), 216-227.

+ Open protocol

+ Expand

Chromatin Immunoprecipitation and Transcription Factor Regulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

General tissue culture materials were obtained from VWR International. Antibodies against Snail and BRD4 were obtained from Abcam. Antibodies against c-MYC, p21 and FOSL1 were purchased from Cell Signaling, HMGA2 antibody was from Biocheck Inc, while vimentin antibody was from Abcam. Alpha-tubulin antibody was obtained from Santa Cruz, while E-cadherin antibody was from BD Bioscience. Secondary antibodies were purchased from Sigma. The EZ-Chip and EZ-Zyme Chromatin Prep kits were from Millipore. The anti-BRD4 antibody for ChIP assay was purchased from Bethyl Laboratories, while the anti-RNA polymerase II antibody and control IgG antibody were from Millipore. BET inhibitor JQ1 was obtained from BPS Bioscience, while I-BET151 was acquired from Tocris Bioscience. BRD4, c-MYC, and FOSL1 siRNAs were purchased from Life Technologies.

Sahai V., Kumar K., Knab L.M., Chow C.R., Raza S.S., Bentrem D.J., Ebine K, & Munshi H.G. (2014). BET bromodomain inhibitors block growth of pancreatic cancer cells in three-dimensional collagen. Molecular cancer therapeutics, 13(7), 1907-1917.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!