Log In Sign up
The largest database of trusted experimental protocols

P eif4e

Manufactured by Abcam
Visit Supplier
Sourced in United States, United Kingdom

P-eIF4E is a recombinant protein that represents the phosphorylated form of the eukaryotic translation initiation factor 4E (eIF4E). eIF4E is a key component of the eukaryotic translation initiation complex and plays a crucial role in regulating protein synthesis.

Automatically generated - may contain errors

Lab products found in correlation

Eif4e, by Abcam (3 mentions) β actin, by Abcam (2 mentions) β actin, by Cell Signaling Technology (2 mentions) Erk1 2, by Cell Signaling Technology (2 mentions) P erk1 2, by Cell Signaling Technology (2 mentions) Mini protean tgx stain free gel, by Bio-Rad (2 mentions) Spark plate reader, by Tecan (2 mentions) Eif4g, by Cell Signaling Technology (2 mentions) Gapdh, by Cell Signaling Technology (2 mentions) Chemidoc mp imaging system, by Bio-Rad (2 mentions) P eif4g, by Cell Signaling Technology (2 mentions) Alphalisa surefire ultra p eif4e ser209 assay kits, by PerkinElmer (2 mentions) P mnk1, by Cell Signaling Technology (2 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) Cleaved parp, by Cell Signaling Technology (1 mentions) P stat3, by Cell Signaling Technology (1 mentions) P src, by Cell Signaling Technology (1 mentions) Eif4e, by BD (1 mentions) Cryptotanshinone, by Yuanye Bio-Technology (1 mentions) Dasatinib, by Yuanye Bio-Technology (1 mentions)

Spelling variants of this product

EIF4E (10 citations) P-eIF4E antibody (3 citations)

7 protocols using p eif4e

1

Western Blot Analysis of Apoptosis Regulators

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies directed against bcl2, Mcl-1 (Mcl-1L), Mnk1, Mnk2, eIF4E, and phosphorylated eIF4E at Ser209 (p-eIF4E), obtained from Abcam, were used to determine protein level by Western blot. β-actin (Abcam) was used as a loading control.
Chen K., Chen Y., Chen Z., Shi Y., He Z., Ding B., Wang C, & Yu L. (2018). miR-134 increases the antitumor effects of cytarabine by targeting Mnks in acute myeloid leukemia cells. OncoTargets and therapy, 11, 3141-3147.
+ Open protocol
+ Expand
2

Evaluation of Cryptotanshinone in Leukemia

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cryptotanshinone (CPT) was purchased from Shanghai Yuanye Biotechnology Co., Ltd, with a purity of 98% (#B21586, Shanghai, China). High-performance liquid chromatography (HPLC) analysis was applied to further confirm the purity of CPT (98%). Imatinib (Genike) was obtained from Nanjing Chia Tai Tianqing Pharmaceutical Co., Ltd. The second-generation tyrosine kinase inhibitors nilotinib was purchased from Aladdin Chemistry Co., Ltd (#N126111, Shanghai, China). Dasatinib was obtained from Shanghai Yuanye Biotechnology Co., Ltd (#S45672, Shanghai, China). Antibodies used in this study were purchased from Cell Signalling Technology (MA, USA), including cleaved caspase-3 (#9664), cleaved PARP (#5625), p-STAT3 (#9145), Bcr-Abl (#3908), p-Bcr-Abl (#3009), p-Src (#2101) and β-Actin (#3700). Antibody for cleaved caspase-9 (Asp353) (#AF5240) was obtained from Affinity Biosciences (Cincinnati, OH, USA), for eIF4E (#610270) was obtained from BD Biosciences (San Diego, CA, USA), and p-eIF4E (ab76256) was purchased from Abcam. The rabbit polyclonal antibody against Ki-67 (PB9026) and mouse monoclonal antibody against PCNA (BM0104) was obtained from Boster Biological Technology Co., Ltd.
Cheng R., Huang Y., Fang Y., Wang Q., Yan M, & Ge Y. (2021). Cryptotanshinone enhances the efficacy of Bcr-Abl tyrosine kinase inhibitors via inhibiting STAT3 and eIF4E signalling pathways in chronic myeloid leukaemia. Pharmaceutical Biology, 59(1), 891-901.
+ Open protocol
+ Expand
3

Quantification of eIF4E Phosphorylation in Neurons

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cortical neurons and brain tissue were homogenized in lysis buffer containing NaCl 137 mM, KCl, 2.7 mM, Na2HPO4 10 mM, KH2PO4, 1.8 mM, EDTA 5 mM, Triton 1%, and complete protease and phosphatase inhibitors (Roche Applied Science). Immunoblotting was done with HRP-conjugated secondary antibodies and Pierce ECL Western Blotting Substrate. The following primary antibody was used in this study: p-eIF4E (Abcam, ab76256 1:1000), eIF4E (Abcam, ab47482 1:1000), p-ERK1/2 (Cell Signaling, 4370S 1:1000), ERK1/2 (Cell Signaling, 4695S 1:1000), p-eIF4G (Cell Signaling, 2441S 1:1000), eIF4G (Cell Signaling, 2498 1:1000), p-MNK1 (Cell Signaling, 2111, 1:1000), MNK1 (Cell Signaling, 2195S, 1:1000), GAPDH (Cell Signaling, 5174 1:2000) and calnexin (Stressgen, SPA-865 1:2000). Loading controls were run on the same gel, and for some experiments Mini PROTEAN® TGX Stain-Free™ Gels (Bio-Rad) were used as loading controls. Signals were acquired using an image analyzer (Bio-Rad, ChemiDoc MP Imaging System) and images were analyzed and prepared using ImageJ.
For additional measurements of eIF4E phosphorylation state, the AlphaLISA SureFire Ultra p-eIF4E (Ser209) Assay Kits (PerkinElmer) were used according to the manufactures protocol. AlphaLISA signals were measured using a Tecan SPARK plate reader on recommended settings.
Hörnberg H., Perez-Garci E., Schreiner D., Hattstatt-Burklé L., Magara F., Baudouin S., Matter A., Nacro K., Pecho-Vrieseling E, & Scheiffele P. (2020). Rescue of oxytocin response and social behavior in a rodent model of autism. Nature, 584(7820), 252-256.
+ Open protocol
+ Expand
4

Quantification of eIF4E Phosphorylation in Neurons

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cortical neurons and brain tissue were homogenized in lysis buffer containing NaCl 137 mM, KCl, 2.7 mM, Na2HPO4 10 mM, KH2PO4, 1.8 mM, EDTA 5 mM, Triton 1%, and complete protease and phosphatase inhibitors (Roche Applied Science). Immunoblotting was done with HRP-conjugated secondary antibodies and Pierce ECL Western Blotting Substrate. The following primary antibody was used in this study: p-eIF4E (Abcam, ab76256 1:1000), eIF4E (Abcam, ab47482 1:1000), p-ERK1/2 (Cell Signaling, 4370S 1:1000), ERK1/2 (Cell Signaling, 4695S 1:1000), p-eIF4G (Cell Signaling, 2441S 1:1000), eIF4G (Cell Signaling, 2498 1:1000), p-MNK1 (Cell Signaling, 2111, 1:1000), MNK1 (Cell Signaling, 2195S, 1:1000), GAPDH (Cell Signaling, 5174 1:2000) and calnexin (Stressgen, SPA-865 1:2000). Loading controls were run on the same gel, and for some experiments Mini PROTEAN® TGX Stain-Free™ Gels (Bio-Rad) were used as loading controls. Signals were acquired using an image analyzer (Bio-Rad, ChemiDoc MP Imaging System) and images were analyzed and prepared using ImageJ.
For additional measurements of eIF4E phosphorylation state, the AlphaLISA SureFire Ultra p-eIF4E (Ser209) Assay Kits (PerkinElmer) were used according to the manufactures protocol. AlphaLISA signals were measured using a Tecan SPARK plate reader on recommended settings.
Hörnberg H., Perez-Garci E., Schreiner D., Hattstatt-Burklé L., Magara F., Baudouin S., Matter A., Nacro K., Pecho-Vrieseling E, & Scheiffele P. (2020). Rescue of oxytocin response and social behavior in a rodent model of autism. Nature, 584(7820), 252-256.
+ Open protocol
+ Expand
5

Immunofluorescence and Western Blot Antibodies

Check if the same lab product or an alternative is used in the 5 most similar protocols
The antibodies used for immunofluorescence staining were as follows: p-eIF4E from Abcam(Cambridge, MA), eIF4E, nucleolin, SUMO-1 and Ki-67 were from Cell Signaling Technology (Beverly, MA).
The antibodies used for western blot analysis were as follows: p-eIF4E, eIF4E, nucleolin, myosin-9, LRP-130, CRM-1, DDX39B, eIF4A1, Tubulin, β-actin, hnRNPA1, SUMO1, SUMO2/3 and Histone-2A antibodies. All antibodies except p-eIF4E antibody (Abcam, Cambridge, MA) were purchased from Cell Signaling Technology (Beverly, MA).
Zhou H., Zhang J., Gu Y., Gan X., Gan Y., Zheng W., Kim B.W., Xu X., Lu X., Dong Q., Zheng S., Huang W, & Xu R. (2015). Identification of a novel RNA giant nuclear body in cancer cells. Oncotarget, 7(4), 4724-4734.
+ Open protocol
+ Expand
6

Immunoblot Analysis of Signaling Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies used for immunoblots were MNK2 (1:300 dilution; Abcam), eIF4E (1:5000 dilution; Abcam), P-eIF4E (1:500 dilution; Abcam), ERK1/2 (1:1000 dilution; Abcam), P-ERK1/2 (1:1000 dilution; Abcam), 4EBP1 (1:1000 dilution; Cell Signaling Technology), P-4EBP1 (1:1000 dilution; Cell Signaling Technology), AKT (1:1000 dilution; Affinity), P-AKT (1:1000 dilution; Affinity), β-actin (1:5000 dilution; Abcam), and Vinculin (1:5000 dilution; Abcam). AnnexinV/PI (KeyGEN). MER Inhibitor PD 0325901 and AKT inhibitor BEZ 235 were purchased from Selleck Chemicals.
Guo Z., Peng G., Li E., Xi S., Zhang Y., Li Y., Lin X., Li G., Wu Q, & He J. (2017). MAP kinase-interacting serine/threonine kinase 2 promotes proliferation, metastasis, and predicts poor prognosis in non-small cell lung cancer. Scientific Reports, 7, 10612.
+ Open protocol
+ Expand
7

Immunohistochemical Analysis of Tumor Tissues

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Tumor specimens from the mice were washed with 1× phosphate buffer solution (PBS), fixed with 4% formaldehyde (Mallinckrodt Chemical Co, St Louis, MO, USA) in PBS, and embedded in paraffin wax. Sections (4 µm) were cut and deparaffinized with antibodies for cleaved caspase-3 (Asp175; Cell Signaling Technology, #9664), p-rpS6 (Ser235/236; Cell Signaling Technology, #2211), p-eIF4E (Ser209; Abcam, Cambridge, UK, ab76256), p-ATM (Ser1981; Abcam, Cambridge, UK, ab81292), p-DNA-PKcs (Ser2056; Abcam, ab18192), 53BP1 (Cell Signaling Technology, #4937), VEGF-A (GeneTex, GTX102643), and HIF-1α (Abcam, ab82832); those antibodies were used as primary antibodies in the indirect immunoperoxidase immunohistochemistry method [28 (link),69 (link),70 (link)].
Chen Y.H., Wang C.W., Wei M.F., Tzeng Y.S., Lan K.H., Cheng A.L, & Kuo S.H. (2019). Maintenance BEZ235 Treatment Prolongs the Therapeutic Effect of the Combination of BEZ235 and Radiotherapy for Colorectal Cancer. Cancers, 11(8), 1204.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!