Fluorescent probe 2,7-dichlorofluorescein diace-tate (DCF-DA), 3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium (MTT), Triton X-100, FBS and Rhodamine-123 were purchased from Sigma(St Louis, MO, USA). Dulbecco’s modified Eagle’s medium (DMEM-F12) was purchased from Gibco (Gibco, Grand Island, NY, USA). Caspase-3 Detection Kit was provided from Sigma. Super Oxide Dismutase Assay Kits were purchased from Cayman (Ann Arbor, MI, USA). LiChroprep® RP-18 (15-25 μm) were purchased from Merck (Darmstadt, Germany) and all the solvents used for extraction from Scharlau and Caledon (Sentmenate, Spain).
Caspase 3 detection kit
Manufactured by Merck Group
Sourced in United States
The Caspase 3 Detection Kit is a laboratory tool used to detect and measure the activity of the caspase-3 enzyme. Caspase-3 is a key mediator of apoptosis, a process of programmed cell death. The kit provides a quantitative assessment of caspase-3 activity, which can be useful in various research applications involving the study of cell death and apoptosis.
Automatically generated - may contain errors
Lab products found in correlation
Triton x 100, by Merck Group (3 mentions)
Fetal bovine serum (fbs), by Merck Group (2 mentions)
Rhodamine 123, by Merck Group (2 mentions)
Dmem f12, by Thermo Fisher Scientific (2 mentions)
Dulbecco s modified eagle s medium dmem f12, by Thermo Fisher Scientific (1 mentions)
Super oxide dismutase assay kits, by Cayman Chemical (1 mentions)
Folin ciocalteu s phenol reagent, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Sodium carbonate, by Merck Group (1 mentions)
Chloroform, by Merck Group (1 mentions)
Doxorubicin, by Merck Group (1 mentions)
Ascorbic acid, by Avantor (1 mentions)
Ferrous chloride, by Merck Group (1 mentions)
Linoleic acid, by Cayman Chemical (1 mentions)
Tween 40, by Merck Group (1 mentions)
Gallic acid, by Cayman Chemical (1 mentions)
Ferrozine iron reagent, by Thermo Fisher Scientific (1 mentions)
Superoxide dismutase assay kit, by Cayman Chemical (1 mentions)
Lichroprep rp 18, by Merck Group (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
7 protocols using caspase 3 detection kit
1
Oxidative Stress and Apoptosis Assay
2
Antioxidant and Cytotoxicity Evaluation
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All chemicals, reagents and kits that were used in this study were purchased according to the following description: Fluorescent probe 2,7-dichlorofluorescein diacetate (DCF-DA), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium (MTT), Triton X-100, FBS and rhodamine-123, 1,1-diphenyl-2-picryl-hydrazyl (DPPH), β-carotene, doxorubicin and caspase-3 detection kit from Sigma, Aldrich (St Louis, MO, USA); DMEM-F12 from Gibco (Gibco, Grand Island, NY, USA); super oxide dismutase assay kit from Cayman; gallic acid, linoleic acid, sodium carbonate, ferrous chloride, dimethyl sulfoxide (DMSO), chloroform, EDTA, Tween® 40, Folin-Ciocalteu’s phenol reagent, butylatedhydroxyltoluene (BHT), LiChroprep® RP-18 (15-25 µm) from Merck; ascorbic acid from VWR; ferrozine iron reagent from Acros; organics and all the solvents used for extraction and fractionation from Scharlau (Sentmenate, Spain).
3
Syk/JAK Inhibition Impacts DLBCL Survival
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Example 339
Data represent two independent experiments to evaluate the effect of Syk and Syk/JAK inhibition on survival of diffuse large non-Hodgkin's lymphoma B cell lines. SUDHL-4 and SUDHL-6 cells lines rely on Syk-mediated B cell receptor signaling for survival, while Toledo cells do not. Cells were incubated with compounds at the indicated concentrations and times; induction of apoptosis was measured by flow cytometry using the Caspase 3 Detection Kit (Sigma-Aldrich, Saint Luis, Mo.). Data are presented as the percent of total cells positive for the apoptosis marker, caspase 3. As expected, Syk inhibition resulted in the induction of apoptosis in SUDHL-4 and -6 cell lines, but not the Toledo cell line.
4
Syk Inhibition Induces Apoptosis
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Example 339
Data represent two independent experiments to evaluate the effect of Syk and Syk/JAK inhibition on survival of diffuse large non-Hodgkin's lymphoma B cell lines. SUDHL-4 and SUDHL-6 cells lines rely on Syk-mediated B cell receptor signaling for survival, while Toledo cells do not. Cells were incubated with compounds at the indicated concentrations and times; induction of apoptosis was measured by flow cytometry using the Caspase 3 Detection Kit (Sigma-Aldrich, Saint Luis, Mo.). Data are presented as the percent of total cells positive for the apoptosis marker, caspase 3. As expected, Syk inhibition resulted in the induction of apoptosis in SUDHL-4 and -6 cell lines, but not the Toledo cell line.
5
Oridonin-induced apoptosis in laryngeal cancer
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Oridonin (C20H28O6), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and 4-phenylbutyric acid (4-PBA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). They were both dissolved with dimethyl sulfoxide (DMSO). FITC Annexin V Apoptosis Detection Kit was purchased from BD (Bioscience, San Jose, CA, USA). Z-VAD-fmk (a caspase-3 inhibitor) was obtained from Selleckchem. Caspase 3 detection kit was purchased from Sigma-Aldrich. Antibodies against cleaved caspase-3, cleaved PARP, GRP 78, phosphorylated-PERK (p-PERK), PERK, phosphorylated-eIF2α (p- eIF2α), eIF2α, CHOP and β-actin were obtained from Cell Signaling Technology (Beverly, MA, USA).
Human laryngeal carcinoma cell lines Hep-2, TU212 were purchased from the American Type Culture Collection (Manassas, VA, USA). All these cell lines were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (Gibco, Grand Island, NY, USA) and maintained at 37°C in a humidified incubator with 5% CO2. As descript previously.
Human laryngeal carcinoma cell lines Hep-2, TU212 were purchased from the American Type Culture Collection (Manassas, VA, USA). All these cell lines were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (Gibco, Grand Island, NY, USA) and maintained at 37°C in a humidified incubator with 5% CO2. As descript previously.
6
Oxidative Stress and Cell Viability Analysis
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Fluorescent probe 2,7-dichlorofluorescein diacetate (DCF-DA), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium (MTT), triton X-100, FBS, and rhodamine 123 were purchased from Sigma (St Louis, MO, USA). DMEM-F12 was purchased from Gibco (Gibco, Grand Island, NY, USA). Caspase-3 Detection Kit was provided from Sigma. Express One-Step SYBR GreenER Kit was purchased from Invitrogen (Carlsbad, CA).
7
Syk and Syk/JAK Inhibition Induces Apoptosis in DLBCL
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Example 339
Data represent two independent experiments to evaluate the effect of Syk and Syk/JAK inhibition on survival of diffuse large non-Hodgkin's lymphoma B cell lines. SUDHL-4 and SUDHL-6 cells lines rely on Syk-mediated B cell receptor signaling for survival, while Toledo cells do not. Cells were incubated with compounds at the indicated concentrations and times; induction of apoptosis was measured by flow cytometry using the Caspase 3 Detection Kit (Sigma-Aldrich, Saint Luis, Mo.). Data are presented as the percent of total cells positive for the apoptosis marker, caspase 3. As expected, Syk inhibition resulted in the induction of apoptosis in SUDHL-4 and -6 cell lines, but not the Toledo cell line.
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