RNA was isolated from different tissues of WT (C57/B6) mice using a Multisource Total RNA Miniprep Kit (Axygen). A reverse-transcription kit (Promega) was used to reverse-transcribe RNA in a 20-µl reaction mixture. Quantification of Aass gene expression was performed using a real-time PCR system (Bio-Rad) in quadruplicate. Gapdh was amplified as the internal control.
A reverse transcription kit
Manufactured by Promega
The reverse-transcription kit is a laboratory tool used to convert RNA into complementary DNA (cDNA) molecules. This process is essential for various applications, such as gene expression analysis and cDNA library construction.
Automatically generated - may contain errors
2 protocols using a reverse transcription kit
1
Quantifying Aass Gene Expression
2
Quantitative Gene Expression Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total RNA was isolated from liver tissues by
Trizol reagent (Dingguo, China). Total RNA
(2 μg) was used to synthesize cDNA using a
reverse transcription kit (Promega). Quantitative
polymerase chain reaction (qPCR) was performed
using SYBR Green (Invitrogen, USA) on a
Rotor-Gene 3000 PCR system (Corbett Robotics,
Australia). β-actin expression was used to
normalize gene expression. Relative mRNA levels
were measured by the means of the 2-ΔΔCt method
(30 (link)). The oligonucleotide primers used are given
inTable 1 .
Trizol reagent (Dingguo, China). Total RNA
(2 μg) was used to synthesize cDNA using a
reverse transcription kit (Promega). Quantitative
polymerase chain reaction (qPCR) was performed
using SYBR Green (Invitrogen, USA) on a
Rotor-Gene 3000 PCR system (Corbett Robotics,
Australia). β-actin expression was used to
normalize gene expression. Relative mRNA levels
were measured by the means of the 2-ΔΔCt method
(30 (link)). The oligonucleotide primers used are given
in
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