Vxr 300

Manufactured by Agilent Technologies

Sourced in United States

The VXR-300 is a high-performance nuclear magnetic resonance (NMR) spectrometer designed for advanced analytical applications. It provides a stable, precise, and reliable platform for conducting NMR experiments. The VXR-300 operates at a magnetic field strength of 7.0 Tesla, enabling researchers to obtain high-resolution NMR data for a wide range of samples.

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Lab products found in correlation

Drx400, by Agilent Technologies (2 mentions) Vxr 400, by Agilent Technologies (2 mentions) Avance drx 500, by Bruker (2 mentions) Gemini 200, by Agilent Technologies (1 mentions) Avance drx 500, by Agilent Technologies (1 mentions) Gemini 300, by Agilent Technologies (1 mentions) B27 supplement, by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Neurobasal medium, by Thermo Fisher Scientific (1 mentions) Dulbecco s modified eagle s media dmem, by Thermo Fisher Scientific (1 mentions) βiii tubulin, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

VXR 300 MHz spectrometer (7 citations) Mercury VXR-300 NMR spectrometer (6 citations) VXR-300 spectrometer (5 citations) Mercury VXR-300 spectrometer (5 citations) VXR 300 MHz (2 citations) Unity VXR-300 (2 citations) Mercury VXR-300 (2 citations)

7 protocols using vxr 300

Standard Organic Synthesis Procedures

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Example 1

All reagents and solvents were purchased from commercial sources. All commercial reagents and solvents were used as received without further purification. The reactions were monitored using analytical thin layer chromatography (TLC) with 0.25 mm EM Science silica gel plates (60F-254). The developed TLC plates were visualized by short wave UV light (254 nm) or immersion in potassium permanganate solution followed by heating on a hot plate. Flash chromatography was performed with Selecto Scientific silica gel, 32-63 μm particle sizes. All reactions were performed in flame or oven-dried glassware under a nitrogen atmosphere. All reactions were stirred magnetically at ambient temperature unless otherwise indicated. ¹H NMR spectra were obtained with a Bruker DRX400, Varian VXR400 or VXR300. ¹H NMR spectra were reported in parts per million (δ) relative to TMS (0.0), DMSO-d₆(2.50) or CD₃OD (4.80) as an internal reference. All ¹H NMR spectra were taken in CDCl₃unless otherwise indicated.

US11485705B2. Salts and prodrugs of 1-methyl-d-tryptophan (2022-11-01). LUMOS PHARMA, INC. [US]. Inventors: Mario Mautino [US], Sanjeev Kumar [US], Firoz Jaipuri [US], Jesse Waldo [US], Hima Potturi [US], Hong Zhuang [US].

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Phosphorus-Hydrogen Compounds Reaction Protocols

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All reactions with P–H compounds were performed under an argon atmosphere. Flash chromatography was carried out using Merck silica gel 60 (230–400 mesh ASTM) and Aldrich ion-exchange resin Dowex WX-50. The NMR spectra were recorded on Varian VXR-300 or Bruker Avance DRX-500 spectrometers for ¹H (TMS); on a Bruker Avance DRX-500 spectrometer for ¹³C {H} (TMS); on Varian Gemini-200 or Varian VXR-300 spectrometers for ¹⁹F (CFCl₃) and for ³¹P (H₃PO₄).

Pavlenko N.V., Oos T.I., Yagupolskii Y.L., Gerus I.I., Doeller U, & Willms L. (2014). A novel family of (1-aminoalkyl)(trifluoromethyl)- and -(difluoromethyl)phosphinic acids – analogues of α-amino acids. Beilstein Journal of Organic Chemistry, 10, 722-731.

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Comprehensive Structural Characterization

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Structural characterization of compounds was carried out by ¹H-NMR, ¹³C-NMR, elemental analysis and mass spectrometry. ¹H-NMR and ¹³C-NMR spectra were obtained at 300 MHz, 400, 500 and 126 MHz and 75 MHz respectively on a VXR-300, a Gemini-300 both Varian Medical System (Palo Alto, CA, USA) or a Bruker (Billerica, MA, USA) 500 MHz spectrometer. Elemental analyses of carbon, hydrogen and nitrogen were performed at the microanalysis laboratory at the University of Illinois (Urbana-Champaign, IL, USA). Mass spectra were obtained on a HP Series 1100 MSD ESI-MS (Hewlett Packard, Palo Alto, CA, USA) electrospray ionization/mass spectrometer,) using a solution of 0.1% formic acid in acetonitrile as solvent injection. Samples were dissolved in acetonitrile (4 mg/mL) prior to injection into the instrument. Melting points were determined on a capillary Thomas-Hoover melting point apparatus (Swedesboro, NJ, USA).

Duque-Benítez S.M., Ríos-Vásquez L.A., Ocampo-Cardona R., Cedeño D.L., Jones M.A., Vélez I.D, & Robledo S.M. (2016). Synthesis of Novel Quaternary Ammonium Salts and Their in Vitro Antileishmanial Activity and U-937 Cell Cytotoxicity. Molecules, 21(4), 381.

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Experimental Procedures for Chemical Synthesis

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Example 1

All reagents and solvents were purchased from commercial sources. All commercial reagents and solvents were used as received without further purification. The reactions were monitored using analytical thin layer chromatography (TLC) with 0.25 mm EM Science silica gel plates (60F-254). The developed TLC plates were visualized by short wave UV light (254 nm) or immersion in potassium permanganate solution followed by heating on a hot plate. Flash chromatography was performed with Selecto Scientific silica gel, 32-63 Lm particle sizes. All reactions were performed in flame or oven-dried glassware under a nitrogen atmosphere. All reactions were stirred magnetically at ambient temperature unless otherwise indicated. ¹H NMR spectra were obtained with a Bruker DRX400, Varian VXR400 or VXR300. ¹H NMR spectra were reported in parts per million (δ) relative to TMS (0.0), DMSO-d₆(2.50) or CD₃OD (4.80) as an internal reference. All ¹H NMR spectra were taken in CDCl₃unless otherwise indicated.

US10207990B2. Salts and prodrugs of 1-methyl-D-tryptophan (2019-02-19). NewLink Genetics, Corp. [US]. Inventors: Mario Mautino [US], Sanjeev Kumar [US], Firoz Jaipuri [US], Jesse Waldo [US], Hima Potturi [US], Hong Zhuang [US].

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Synthesis and Characterization of CPH:SA Copolymer

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Synthesis of 20:80 CPH:SA copolymer was performed as described previously (36 , 39 (link)). Proton NMR (VXR-300, Varian) was used to measure polymer molecular weight and purity. Quantum dots (QDs, λ_ex = 554 nm, λ_em = 627 nm) were purchased from Sigma-Aldrich. Neurobasal medium, RPMI 1640 and Dulbecco’s modified Eagle’s media (DMEM), B27 supplement, fetal bovine serum (FBS), Trypsin-EDTA (TE), L-glutamine, penicillin, and streptomycin were purchased from Invitrogen. The MTS cell viability kit (Catalog# G3580) was acquired from Promega. Primary antibodies against cleaved caspase-3 (Catalog# 9661) and β-III tubulin (Catalog# 14545) were purchased from Cell Signaling and Millipore, respectively.

Brenza T.M., Ghaisas S., Vela Ramirez J.E., Harischandra D., Anantharam V., Kalyanaraman B., Kanthasamy A.G, & Narasimhan B. (2016). Neuronal Protection against Oxidative Insult by Polyanhydride Nanoparticle-based Mitochondria-targeted Antioxidant Therapy. Nanomedicine : nanotechnology, biology, and medicine, 13(3), 809-820.

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Synthesis and Characterization of CPH-SA Copolymers

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Synthesis of sebacic anhydride (SA) and 1,6-bis(p-carboxyphenoxy)hexane (CPH) pre-polymers and copolymers was performed as previously described.^{27 (link), 28} The resulting copolymer of 20 mol percent CPH and 80 mol percent SA (i.e., 20:80 CPH:SA) was characterized using ¹H nuclear magnetic resonance spectroscopy (VXR-300, Varian, Palo Alto, CA) to verify copolymer composition and molecular weight. All properties of the synthesized copolymers were within expected ranges.^{27 (link), 28}

Brenza T.M., Schlichtmann B.W., Bhargavan B., Ramirez J.E., Nelson R.D., Panthani M.G., McMillan J.M., Kalyanaraman B., Gendelman H.E., Anantharam V., Kanthasamy A.G., Mallapragada S.K., Narasimhan B, & Kanmogne G.D. (2018). Biodegradable polyanhydride-based nanomedicines for blood to brain drug delivery. Journal of biomedical materials research. Part A, 106(11), 2881-2890.

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NMR Spectroscopy and Chromatography Protocol

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¹H NMR spectra were recorded with a Varian VXR-300 (299.9 MHz) spectrometer. ¹³C NMR spectra were recorded with a Bruker Avance DRX 500 (125.75 MHz) spectrometer. Chemical shifts (δ) are given in ppm downfield relative to internal tetramethylsilane (TMS) for ¹H and ¹³C. Chromatography was performed on Gerudan SI 60 silica gel. Elemental analyses were performed at the analytical laboratory of the Institute of Organic Chemistry, National Academy of Sciences in Ukraine. The analytical grade solvents and commercially available reagents were used without further purification. Melting points were determined with an electrothermal capillary melting point apparatus.

Costa de Oliveira R., Soares Pontes G., Kostyuk A., Coutinho Camargo G.B., Dhyani A., Shvydenko T., Shvydenko K, & Grafov A. (2020). Anticancer and Immunomodulatory Activities of a Novel Water-Soluble Derivative of Ellipticine. Molecules, 25(9), 2130.

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