Prior to plasma exposure, the entire volume of growth medium was aspirated. One hundred μg gWizGFP (Aldevron, Fargo, ND, USA), was carefully layered over the cells in 100 μl of phosphate buffered saline (PBS). Cells were exposed to the plasma at a 2 mm or 5 mm distance for 5 seconds (sec) for each experiment. Immediately after plasma irradiation, 5 ml of complete growth medium was layered over the cells, and the slides were placed in the incubator for an additional 24 hours.
We also investigated the influence of the electric field on plasma-induced GFP expression. The glass slide was flipped over so other processes such as exposure to reactive species could not reach the target cells. For these experiments, the distance between cell surface and plasma was maintained at 2 mm at a voltage of 11.25 kV.
We also investigated the influence of the electric field on plasma-induced GFP expression. The glass slide was flipped over so other processes such as exposure to reactive species could not reach the target cells. For these experiments, the distance between cell surface and plasma was maintained at 2 mm at a voltage of 11.25 kV.