Cd38 bv510

Manufactured by BD

CD38-BV510 is a fluorochrome-conjugated antibody that binds to the CD38 surface antigen. It is designed for use in flow cytometry applications to identify and characterize CD38-expressing cells.

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Lab products found in correlation

Cd19 apc cy7, by BD (1 mentions) Cd27 fitc, by BD (1 mentions) Cd24 bv421, by BD (1 mentions) Anti cd16 32 fc block, by BioLegend (1 mentions) Anti cd11c allophycocyanin apc, by BD (1 mentions) Collagenase d, by Roche (1 mentions) Gallios flow cytometer, by Beckman Coulter (1 mentions) F4 80 apc cy7, by BioLegend (1 mentions) Anti f4 80 apc, by BD (1 mentions) Tlr2 apc, by BioLegend (1 mentions) Fc block, by Thermo Fisher Scientific (1 mentions) Fortessa analyzer, by BD (1 mentions) Cd19 bv711, by BD (1 mentions) Zombie violet fixable viability kit, by BioLegend (1 mentions) Cd45ra pe, by BD (1 mentions) Cd115 apc, by BioLegend (1 mentions) Cd45 a700, by BioLegend (1 mentions) Cd24 pe cf594, by BD (1 mentions)

3 protocols using cd38 bv510

Immunophenotyping of PBMCs in RRMS

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For cross-sectional study, fresh peripheral blood mononuclear cells (PBMCs) were surface stained with monoclonal antibodies against CD19-APC-cy7, CD27-FITC, CD24-BV421, CD38-BV510, and PD-L1(CD274)-PE-cy7 (BD Biosciences). For longitudinal study, frozen PBMCs, collected from 11 RRMS patients undergoing alemtuzumab at baseline and 6, 9, and 12 months, were surface stained with monoclonal antibodies as stated above.

Kim Y., Kim G., Shin H.J., Hyun J.W., Kim S.H., Lee E, & Kim H.J. (2018). Restoration of regulatory B cell deficiency following alemtuzumab therapy in patients with relapsing multiple sclerosis. Journal of Neuroinflammation, 15, 300.

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Isolation of Splenic Dendritic Cells and Macrophages

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For isolation of splenic dendritic cells and macrophages, mouse spleens were perfused with 400 U/ml of collagenase D (Roche, Basel, Switzerland) in Hanks' balanced salt solution and incubated for 45 min at 37° followed by mechanical dissociation. Splenocytes were first incubated with anti‐CD16/32 (Fc‐block) (Biolegend, San Diego, CA) in PBS [1 mm EDTA, 2% fetal calf serum (FCS)] at 4° for 15 min, and were then stained with anti‐CD11c‐allophycocyanin (APC) (BD Biosciences, San Jose, CA) or anti‐F4/80‐APC (BD Biosciences, San Jose, CA) at 4° in PBS with 1 mm EDTA, 2% FCS. The BMDMs were first incubated with anti‐CD16/32 (Fc‐block) (Biolegend, San Diego, CA) in PBS (1 mm EDTA, 2% FCS) at 4° for 15 min. Cells were then stained with the following panel for 30 min at 4° in PBS (1 mm EDTA, 2% FCS): TLR2‐APC (Biolegend, San Diego, CA), CD206‐phycoerythrin/Cy7 (Biolegend, San Diego, CA), CD38‐BV510 (BD Biosciences, San Jose, CA) and F4/80‐APC/Cy7 (Biolegend, San Diego, CA). After washing twice, the cells were acquired using a Gallios flow cytometer (Beckman Coulter, Brea, CA) followed by data analysis using flowjo v10 (FlowJo, Ashland, OR).

Sjöstrand M., Carow B., Nyberg W.A., Covacu R., Rottenberg M.E, & Espinosa A. (2019). TRIM21 controls Toll‐like receptor 2 responses in bone‐marrow‐derived macrophages. Immunology, 159(3), 335-343.

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Multiparameter Flow Cytometry Immunophenotyping

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Single-cell suspensions were incubated with human Fc receptor-binding inhibitor (Fc Block, eBioscience) before surface staining with anti-human monoclonal antibodies (mAbs). Fluorescence minus one (FMO) controls with isotype controls were used to define positive signals for flow cytometry or cell sorting. Dead cells were excluded with the Zombie Violet Fixable Viability Kit (Biolegend).
For labeling, cells were resuspended in PBS, 2% FCS (1 to 5 x10 7 cells/500 ml) and incubated with the following mAbs: CD45 A700 (Biolegend, clone HI30), CD34 PB (Biolegend, clone 581), CD38 BV510 (BD Bioscience, clone HIT2), CD117 BV605 (Biolegend, clone 104D2), CD45RA PE (BD Bioscience, clone HIT100), CD7 FITC (Beckman Coulter, clone 8H8.1), CD2 PerCPCy5.5 (Biolegend, clone RPA2.10), CD115 APC (Biolegend, clone 9-4D2-1E4), CD116 APC-vio770 (Miltenyi, clone REA211), CD123 BV786 (BD Bioscience, clone 7G3), CD127 PC5 (Biolegend, clone A019D5), ITGB7 PC7 (eBioscience, clone FIB504), CD10 BV650 (BD bioscience, clone HI10a), CD19 BV711 (BD bioscience, clone HIB19), CD24 PE-CF594 (BD bioscience, clone ML5). Flow cytometry analyses and cell sorting were performed with a BD Fortessa Analyzer or a BD FACSAria IIII sorter (BD Biosciences; (purity R 95%).

Alhaj Hussen K., Vu Manh T.P., Guimiot F., Nelson E., Chabaane E., Delord M., Barbier M., Berthault C., Dulphy N., Alberdi A.J., Burlen-Defranoux O., Socié G., Bories J.C., Larghero J., Vanneaux V., Verhoeyen E., Wirth T., Dalod M., Gluckman J.C., Cumano A, & Canque B. (2017). Molecular and Functional Characterization of Lymphoid Progenitor Subsets Reveals a Bipartite Architecture of Human Lymphopoiesis. Immunity, 47(4).

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