Nsaid 6 clips

[1] Blood chemistry. Whole blood (400–600 μL) was drawn from a part of the mice used or prepared in the tolerability study described above (vehicle, n = 4; all other groups, n = 3) using S-Monovette^® charged with serum gel (1.1 mL syringe, Sarstedt) and allowed to clot at room temperature for 30–40 min. After centrifugation at 2,000 × g for 10 min, resulting serum samples (150 μL) were loaded onto NSAID 6 clips specialized for identifying liver damage (IDEXX, Westbrook, ME) and analyzed using a Catalyst Dx Chemistry Analyzer (IDEXX). [2] Histology. Paraffin-embedded liver sections were prepared using the fixed livers and then stained using hematoxylin and eosin (H&E). Subsequently, bright-field images of the H&E-stained tissues (×20) were taken using a Nikon Eclipse Ti microscope. Images were processed using NIS-Element software (version 4.51.00) and analyzed using Image J. [3] Hematology. whole blood (700–1,000 μL) was drawn from a part of the mice used in the tolerability study described above (n = 4 for all groups) using S-Monovette^® charged with K₃ EDTA (1.1 mL syringe, Sarstedt). Blood samples were gently mixed well by inversion and stored on ice until analysis (for less than 4 h). Each blood sample (500 μL) was analyzed using a Procyte Dx^® (IDEXX).