Kif4a

Manufactured by Fortis Life Sciences

KIF4A is a protein that functions as a microtubule-based molecular motor involved in chromosomal and midzone microtubule organization during cell division. It plays a crucial role in the regulation of chromosome condensation and segregation, as well as the formation and function of the mitotic spindle.

Automatically generated - may contain errors

Lab products found in correlation

Actin, by Santa Cruz Biotechnology (2 mentions) Kif20a, by Fortis Life Sciences (2 mentions) Sc 867, by Santa Cruz Biotechnology (2 mentions) Kif23, by Santa Cruz Biotechnology (2 mentions) Kifc1, by Cell Signaling Technology (2 mentions) Kif14, by Fortis Life Sciences (2 mentions) Axin1, by Cell Signaling Technology (1 mentions) Rabbit anti egfr, by Cell Signaling Technology (1 mentions) Sheep anti mouse hrp, by GE Healthcare (1 mentions) Donkey anti rabbit hrp, by GE Healthcare (1 mentions) Mouse anti gapdh, by Merck Group (1 mentions) Bubr1, by Fortis Life Sciences (1 mentions) Pp2a scaffold subunit, by Cell Signaling Technology (1 mentions) Mouse anti transferrin receptor, by Thermo Fisher Scientific (1 mentions) Rabbit anti adam17, by Abcam (1 mentions) Anti gfp, by Roche (1 mentions) α tubulin, by Merck Group (1 mentions) Foxm1, by Santa Cruz Biotechnology (1 mentions) Tubulin, by Merck Group (1 mentions)

3 protocols using kif4a

Antibody Validation for Western Blotting

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies were used at the indicated dilutions: c‐Myc (9E10, sc‐40, 1:1,000, Santa Cruz Biotechnology), Separase (A302‐215A, 1:2,000, Bethyl Laboratories), KIF4A (A301‐074A, 1:1,000, Bethyl Laboratories), Axin1 (#2087, 1:1,000, Cell Signaling Technology), B56α (610615, 1:3,000, BD Biosciences), BubR1 (A300‐995A,1:1,000, Bethyl Laboratories), PP2A catalytic subunit (05‐421, 1:2,000, Millipore), PP2A scaffold subunit (#2041, 1:1,000, Cell Signaling Technology) GFP (ab290, 1:4,000, Abcam), FoxO3 pS413 (#8174, 1:1,000, Cell Signaling Technology), FoxO3 rabbit polyclonal α‐pS253 (Raised against peptide CAPRRRAV(pS)MDNS; 1:500, Moravian Biotechnology), Anti‐RFP (1:1,000; MBL, FM005), Anti‐GFP (1:1,000; Roche, 11814460001), Rabbit anti‐ADAM17 (1:1,000, Abcam, 2051), Rabbit anti‐ADAM17 (1:1,000, Abcam, 39162), Rabbit anti‐EGFR (1:1,000, cell signaling, 2232), Rabbit anti‐pEGFR Y1068 (1:1,000, cell signaling, 2234), Mouse anti‐Transferrin receptor (1:1,000, Invitrogen, 136800), Mouse anti‐GAPDH (1:5,000, Sigma, G8795), rabbit Anti‐GFP (1:3,000, Takara Bio Clontech, 632592), Donkey anti‐rabbit‐HRP (1:2,000, GE Healthcare, NA934), Sheep anti‐mouse‐HRP (1:2,000, GE Healthcare, NXA931), and H3pS10 (06‐570, 1:1,000, Millipore).

Kruse T., Gnosa S.P., Nasa I., Garvanska D.H., Hein J.B., Nguyen H., Samsøe‐Petersen J., Lopez‐Mendez B., Hertz E.P., Schwarz J., Pena H.S., Nikodemus D., Kveiborg M., Kettenbach A.N, & Nilsson J. (2020). Mechanisms of site‐specific dephosphorylation and kinase opposition imposed by PP2A regulatory subunits. The EMBO Journal, 39(13), e103695.

+ Open protocol

+ Expand

Antibody Panel for Cell Cycle Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following primary antibodies were used: Actin: sc-47778 (Santa Cruz Biotechnology). LIN9: ab62329 (Abcam). B-MYB: sc-724 (Santa Cruz Biotechnology) and LX015.1 (kind gift from Roger Watson (Tavner et al., 2007). FOXM1: sc-502 (Santa Cruz Biotechnology), KIF14: A300-912A (Bethyl Laboratories). KIF4A: A301-074A (Bethyl Laboratories). KIF20A: A300-879A/Bethyl Laboratories). KIF23: sc-867 (Santa Cruz Biotechnology). KIF2C: kind gift from Linda Wordeman. KIFC1: 12313 (Cell Signalling). PRC1: sc-8356 (Santa Cruz Biotechnology). α-tubulin (Sigma T6074).

Wolter P., Hanselmann S., Pattschull G., Schruf E, & Gaubatz S. (2017). Central spindle proteins and mitotic kinesins are direct transcriptional targets of MuvB, B-MYB and FOXM1 in breast cancer cell lines and are potential targets for therapy. Oncotarget, 8(7), 11160-11172.

+ Open protocol

+ Expand

Mitotic Kinesin Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following primary antibodies were used: Actin: sc-47778 (Santa Cruz Biotechnology). KIF4A: A301-074A (Bethyl Laboratories). PRC1: sc-8356 (Santa Cruz Biotechnology). KIF14: A300-912A (Bethyl Laboratories). KIF20A: A300-879A/Bethyl Laboratories). KIF23: sc-867 (Santa Cruz Biotechnology). KIF2C: kind gift from Linda Wordeman. KIFC1: 12313 (Cell Signalling). ɑ-tubulin (Sigma T6074).

Hanselmann S., Wolter P., Malkmus J, & Gaubatz S. (2017). The microtubule-associated protein PRC1 is a potential therapeutic target for lung cancer. Oncotarget, 9(4), 4985-4997.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!