Fitc conjugated anti human cd11b

To determine the expression of mesenchymal stem cell markers on T-MSCs, cells were collected and pelleted by centrifugation at 1300 rpm for 5 min. After washing with PBS, cells were pelleted again and stained with FITC-conjugated anti-human CD11b, PE-conjugated anti-human CD34, PerCP-conjugated anti-human CD45, APC-conjugated anti-human CD73, PE-conjugated anti-human CD90, or PE-conjugated anti-human CD105 antibody (Biolegend, San Diego, CA, USA) diluted in FACS buffer (PBS supplemented with 10% FBS, 10 mM EDTA, 20 mM HEPES, and 1 mM sodium pyruvate) for 30 min on ice. To determine the expression of megakaryocytic marker on differentiated K562 cells, Pacific Blue-conjugated anti-human CD41 antibody (Biolegend) was used. After washing cells with buffer, surface protein expression was measured using a NovoCyte flow cytometer (ACEA Biosciences, San Diego, CA, USA) and analyzed using NovoExpress software.

Fc receptors in all samples were blocked with TruStain fcX (Biolegend, USA). THP‐M treated with various concentrations of CSE or IL‐4 were incubated with FITC‐conjugated anti‐human CD11b (BioLegend, USA) and APC‐conjugated anti‐human CD206 (Biolegend, USA). FlowJo (FlowJo LLC, USA) was used to test samples obtained from the FACSCanto (BD Biosciences, USA). Cell distribution was expressed as a percentage.