Coulter hmx

Manufactured by Beckman Coulter

Sourced in Italy

The Coulter HMX is a hematology analyzer designed for complete blood count (CBC) testing. It provides an automated method for the measurement and analysis of various blood cell parameters, including red blood cells, white blood cells, and platelets.

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Coulter HmX Hematology Analyzer Coulter HmX AL Hematology Analyzer Coulter HMX Automated Hematology Analyzer

6 protocols using coulter hmx

Hematology analysis of blood samples

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Blood PLT and MPV were measured using the Coulter HmX hematology analyzer (Beckman Coulter, Inc.) on fasting blood samples^{8 (link)}.

Lee D.H., Yao C., Bhan A., Schlaeger T., Keefe J., Rodriguez B.A., Hwang S.J., Chen M.H., Levy D, & Johnson A.D. (2020). Integrative Genomic Analysis Reveals Four Protein Biomarkers for Platelet Traits. Circulation research, 127(9), 1182-1194.

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Comprehensive Blood Count Analysis

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Five mL of venous blood sample was collected from the participant and stored in tubes containing EDTA. A complete blood count analysis was done with automated analyzers Coulter HmX from Beckman Coulter at admission. Hemoglobin (Hb), Red blood cell (RBC) count, white blood cell (WBC) count, lymphocyte count, neutrophil count, platelet count (PLTs), mean platelet volume (MPV), platelet distribution width (PDW), erythrocyte sedimentation rate (ESR), and C-reactive protein (CRP) were recorded for each individual. The reference values in the affiliated Imam Reza laboratory were 3.5–9.5×109/μL for WBC count, 1.8–6.3 ×109/μL for neutrophil count, 40–70% for neutrophil percentage, 181–300 ×109/μL for PLT, 9.4–12.5 fL for MPV and 15.5–18.1% for PDW.

Akya A., Bozorgomid A., Ghadiri K., Ahmadi M., Elahi A., Mozafari H., Almasi A., Namadi P, & Chegenelorestani R. (2020). Usefulness of Blood Parameters for Preliminary Diagnosis of Brucellosis. Journal of Blood Medicine, 11, 107-113.

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Hematological Analyses and Biomarker Measurement

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All hematological analyses were performed by the same cell counter (Coulter HMX, Beckman Coulter, IL Milan, Italy), within 1 h from venipuncture. Soluble P-selectin and von Willebrand factor (VWF) were measured in stored plasma via the Human P-selectin Platinum Enzyme-linked Immunosorbent Assay (ELISA) kit (Affimetrix, eBioscience) [10 (link),21 (link)] and the Hemosil^® von Willebrand Factor Antigen (ELISA kit) (Instrumentation Laboratory), respectively, according to the manufacturer’s instructions. High-sensitivity (hs) C reactive protein (CRP) was measured in serum as described [22 (link)].

Izzi B., Gialluisi A., Gianfagna F., Orlandi S., De Curtis A., Magnacca S., Costanzo S., Di Castelnuovo A., Donati M.B., de Gaetano G., Hoylaerts M.F., Cerletti C, & Iacoviello L. (2021). Platelet Distribution Width Is Associated with P-Selectin Dependent Platelet Function: Results from the Moli-Family Cohort Study. Cells, 10(10), 2737.

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Biochemical Analysis of Blood Coagulation

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Biochemical analyses were performed in the centralized Moli-sani laboratory. Blood samples were obtained between 07:00 and 09:00 from participants who had fasted overnight and had refrained from smoking for at least 6 h. Hematological cytometric analyses were performed by the same cell counter (Coulter HMX, Beckman Coulter, IL Milan, Italy), within 1 h from venipuncture. Platelet–leukocyte conjugates, platelet P-selectin, leukocyte CD11b, and L-selectin expression were measured in whole blood for the Moli-family participants, as described [27 (link)].
Whole blood procoagulant activity was measured by the coagulation time. Whole blood was incubated for 2 h at 37 °C with or without tumor necrosis factor (TNF)-α (100 ng/ml). The optimal agonist concentration was previously selected on the basis of dose-response curves (not shown). At the end of incubation, whole blood coagulation time (i.e., the time taken for recalcified blood to clot) was assessed by a one-stage clotting time. Briefly, 200-μL whole blood were mixed with 100 μL 25 mM CaCl₂, and the time to clot formation was recorded (seconds) [28 (link)].

Noro F., Gianfagna F., Gialluisi A., De Curtis A., Di Castelnuovo A., Napoleone E., Cerletti C., Donati M.B., de Gaetano G., Hoylaerts M.F., Iacoviello L, & Izzi B. (2019). ZBTB12 DNA methylation is associated with coagulation- and inflammation-related blood cell parameters: findings from the Moli-family cohort. Clinical Epigenetics, 11, 74.

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Measuring Leukocyte Counts in NHANES

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To obtain leukocyte counts, blood draws were performed by trained phlebotomists during the participant visit to the MEC. Specific details on venipuncture can be found in the NHANES Laboratory Procedures Manual. Complete blood counts were conducted using the Coulter method (Coulter^® HMX, Beckman Coulter, Brea, California) (CDC, 2013 ). The leukocytes analyzed in this study included total WBC, monocyte, lymphocyte, and neutrophil counts expressed as 1000 per microliter. Additionally, percentages of monocytes, lymphocytes, and neutrophils were examined. Although information on basophils and eosinophils was available, about 95% of participants had values below 0.4 (1000 per μL) for eosinophils and 99% had values below 0.2 (1000 per μL) for basophils. Given the limited range in values for these two leukocyte types, they were excluded from the analyses. All outcomes were treated as continuous measures.

Wirth M.D., Sevoyan M., Hofseth L., Shivappa N., Hurley T.G, & Hébert J.R. (2017). The Dietary Inflammatory Index is Associated with Elevated White Blood Cell Counts in the National Health and Nutrition Examination Survey. Brain, behavior, and immunity, 69, 296-303.

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Fasting Biomarker Profiling Protocol

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All blood samples were obtained from participants who had fasted overnight and had refrained from smoking for at least 6 h. Serum lipids and blood glucose were assayed by enzymatic reaction methods using an automatic analyser (ILab 350; Instrumentation Laboratory). LDL-cholesterol was calculated according to Friedewald (27) .
High-sensitivity (hs) C-reactive protein (CRP) was measured in fresh serum samples within 3 h of collection by a particleenhanced immunoturbidimetric assay (IL-Coagulation-Systems ACL9000; Instrumentation Laboratory). Quality control for hs-CRP was maintained using in-house serum pool and internal laboratory standard at 1•5 mg/l; inter-day CV of hs-CRP were 5•5 and 4•2 %, respectively.
Hemocromocytometric analysis was performed by cell count (Coulter HMX, Beckman Coulter; Instrumentation Laboratory). Neutrophil (granulocyte) to lymphocyte ratio (NLR) was calculated as a marker of low-grade inflammation (22, 28) .

Bonaccio M., Di Castelnuovo A., De Curtis A., Costanzo S., Bracone F., Persichillo M., Donati M.B., de Gaetano G, & Iacoviello L. (2015). Nut consumption is inversely associated with both cancer and total mortality in a Mediterranean population: prospective results from the Moli-sani study. The British journal of nutrition, 114(5).

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