Paclitaxel

Crizotinib, alectinib, ceritinib, paclitaxel, pemetrexed, etoposide, luminespib (AUY922) and lorlatinib (PF-06463922) were purchased from ChemieTek (Indianapolis, IN). Ganetispib was purchased from Selleckchem (Houston, TX). Retaspimycin hydrochloride (IPI504) was purchased from Apexbio (Houston, TX).

Cisplatin, metformin and fenformin were purchased from Sigma-Aldrich (St. Louis, MO, USA); carboplatin from Adipogen (San Diego, CA, USA); paclitaxel from ChemieTek (Indianapolis, IN, USA); doxorubicin and rotenone from Merck; Yondelis (ET-743) from PharmaMar (Madrid, Spain); olaparib from TargetMol (Boston, MA, USA); oxaliplatin, rucaparib, niraparib, KU55933 (ATM inhibitor), AZD6738 (ATR inhibitor), AZD7762 (Chk1 inhibitor) and AZD1775 (Wee1 inhibitor) from Axon Medchem (Groningen, The Netherlands). All the drugs were dissolved in DMSO or water as stock solutions and diluted in medium just before treatment. For cytotoxicity experiments, cells were seeded at 1000–2000 cells/mL and treated with different drug concentrations in 96-well plates 48 h after seeding. After five days of treatment, cell viability was examined with the MTS assay system (Promega Corporation, Madison, WI, USA), and absorbance was acquired using a microplate reader (GloMax Discover, Promega Corporation). Drug concentrations inhibiting growth in 50% of the cells (IC50) were calculated for each cell line, with the interpolation method on Prism 9.5.1 (GraphPad Software, La Jolla, CA, USA). All the experiments were run at least three times in sestuplicate.

Cisplatin was purchased from Sigma Aldrich; paclitaxel from ChemieTek; olaparib from LC Laboratories; VE822, MK1775 and KU55933 from Axon Medchem; THZ1 and THZ1 hydrochloride were from Insight Biotechnology Limited; ET-743 from PharmaMar and PF-00477736 from Pfizer. For cytotoxicity experiments, cell lines were seeded in 96-well plates and were treated after 48 hours with different concentrations of the drugs. After 72 hours cell viability was examined with the MTS assay (Promega) and absorbance was acquired using a plate reader (Infinite M200, TECAN). Drug concentrations inhibiting growth in 50% of the cells (IC50s) were calculated for each cell line, with the interpolation method.