Human chronic myelogenous leukemia K562 cells, human promyelocytic leukemia HL-60 cells, human acute T lymphocytic leukemia Jurkat cells, human acute myeloid leukemia cell line Kasumi-1, human cervical carcinoma HeLa cells, human cervical squamous carcinoma SiHa cells, human cervical cancer cell lines CaSki, human hepatocellular carcinoma BEL-7402, human lung adenocarcinoma cell line A549, human breast cancer MDA-MB-231, human prostate cancer PC-3, mouse hepatoma cell line H22, and murine breast carcinoma cell line 4T1 were obtained from Shanghai Cell Bank, Chinese Academy of Science. The human non-small cell lung cancer cell line NCI-H1975 was provided by the American Type Culture Collection. A549 cell line was cultured in Ham’s F12K medium (F12K) with 10% fetal bovine serum and 1% penicillin/streptomycin. The K562 cell line was cultured in Iscove’s Modified Dulbecco’s medium (IMDM) with 10% fetal bovine serum and 1% penicillin/streptomycin. HeLa cell line was cultured in modified Eagle’s medium (MEM) with 10% fetal bovine serum and 1% penicillin/streptomycin, and others were cultured in Roswell Park Memorial Institute (RPMI)-1640 medium with 10% fetal bovine serum and 1% penicillin/streptomycin. All the cells were cultured at 37 °C with 5% CO2 in a humidified incubator.
Caski
Manufactured by Shanghai Cell Bank
Sourced in China
The CaSki is a laboratory equipment used for cell culture. It is a cervical cancer cell line derived from a human squamous cell carcinoma of the cervix. The CaSki cell line is commonly used in research for various applications, including the study of cervical cancer and related diseases.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Hcerepic, by Shanghai Cell Bank (2 mentions)
Kasumi 1, by Shanghai Cell Bank (1 mentions)
Mda mb 231, by Shanghai Cell Bank (1 mentions)
Hela cells, by Shanghai Cell Bank (1 mentions)
Bel 7402, by Shanghai Cell Bank (1 mentions)
Nci h1975, by American Type Culture Collection (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Merck Group (1 mentions)
Streptomycin, by Merck Group (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
Cisplatin, by Merck Group (1 mentions)
5 protocols using caski
1
Diverse Cancer Cell Line Cultures
2
Cervical Cancer Tissue Collection and Cell Culture
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A total of 50 patients with cervical cancer were recruited into this study at the first affiliated Hospital of Harbin Medical University between January 2013 and September 2015. None of these patients received anti-tumor treatment prior to the surgical resection. Paired cancer tissues and adjacent non-cancerous tissues (>5 cm distant from the cancer tissues) were collected and frozen at −80°C immediately. All procedures performed involving human participants were conducted in accordance with the Declaration of Helsinki. The study was approved by the Medical Ethics Committee of the first affiliated Hospital of Harbin Medical University. Written informed consents were received from all participants.
Human cervical cancer cell lines including Hela, SiHa, Caski, C33A, and the normal human cervical epithelial cell HCerEpiC were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% of fetal bovine serum (FBS; Gibco, Thermo Fisher Scientific, Inc., Waltham, MA, USA), 100 μg/mL streptomycin, and 100 U/mL penicillin (Sigma-Aldrich, Merck KGaA, Darmstadt, Germany). Cells were maintained at 37°C with 5% CO2.
Human cervical cancer cell lines including Hela, SiHa, Caski, C33A, and the normal human cervical epithelial cell HCerEpiC were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% of fetal bovine serum (FBS; Gibco, Thermo Fisher Scientific, Inc., Waltham, MA, USA), 100 μg/mL streptomycin, and 100 U/mL penicillin (Sigma-Aldrich, Merck KGaA, Darmstadt, Germany). Cells were maintained at 37°C with 5% CO2.
3
Culturing Human Cervical Cell Lines
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Human normal cervical epithelial cells (HCerEpiC) and CC cell lines (CaSki, HT-3, C33A and SiHa) were all obtained from Shanghai Cell Bank of the Chinese Academy of Science (Shanghai, China). The cells were cultured in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) containing with 10% fetal bovine serum (FBS; Thermo Fisher Scientific, Waltham, MA, USA) and 1% penicillin-streptomycin (Hyclone, South Logan, UT, USA) at 37°C.
4
Cervical Cancer Tissue and Cell Line Collection
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From 2013 to 2018, 64 pairs of fresh frozen cervical cancer tissues and matched normal tissues were collected at The Third Affiliated Hospital of Zhengzhou University from. No patients had undergone radiotherapy or chemotherapy before surgery. The samples were identified by two pathologists independently. This study was approved by the Institutional Review Boards of The Third Affiliated Hospital of Zhengzhou University, and the samples were conducted in accordance with the Declaration of Helsinki. Informed consent for research purposes was obtained before enrollment.
The cervical cancer cell lines HeLa, SiHa, C33A, CaSki, ME180, MS751 and cervical epithelial cell ECT1/E6E7 were purchased from Shanghai Cell Bank of the Chinese Academy of Science. All cells were cultured in Dulbecco’s Modified Eagle medium (DMEM) medium (HyClone) with 1% penicillin-streptomycin and 10% fetal bovine serum (FBS) (Gibco, USA).
The cervical cancer cell lines HeLa, SiHa, C33A, CaSki, ME180, MS751 and cervical epithelial cell ECT1/E6E7 were purchased from Shanghai Cell Bank of the Chinese Academy of Science. All cells were cultured in Dulbecco’s Modified Eagle medium (DMEM) medium (HyClone) with 1% penicillin-streptomycin and 10% fetal bovine serum (FBS) (Gibco, USA).
5
Establishing Cisplatin-Resistant Cervical Cancer Cell Lines
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Human cervical cancer cell lines CaSki and HeLa were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences (Shanghai, China) and cultured in RPMI‐1640 supplemented with 10% heat‐inactivated fetal bovine serum (Hyclone, Logan, UT, USA). HeLa and CaSki cells were cultured in increasing concentrations of Cisplatin for over 6 months to establish Cisplatin‐resistant (CR) cell lines, HeLa‐CR and CaSki‐CR. All cells were cultured in a humidified atmosphere at 37°C and 5% CO2. Mycoplasma contamination of cell culture was regularly checked every 3 months. Cisplatin, MTT (3‐[4,5‐dimethyl‐2‐yl]‐2,5‐diphenyl tetrazolium bromide) and all other chemicals used in this study were purchased from Sigma (St. Louis, MO) unless stated otherwise.
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