All mice were fasted for 6 h in the morning before blood glucose measurements. Blood glucose was measured through the tail vein by the use of the OneTouch UltraEasy Blood Glucose Monitoring System (Lifescan, Milpitas, CA, USA). Serum insulin levels were determined by a murine enzyme-linked immunosorbent assay kit (Shanghai Enzyme-linked Biotechnology Co., Shanghai, China). Whole blood was withdrawn from the orbital sinus, and plasma was separated by centrifugation at 3400 rpm for 15 min in EDTA-K2-treated microtubes (Kangjian Medical, China).
Onetouch ultraeasy blood glucose monitoring system
Manufactured by LifeScan
Sourced in United States
The OneTouch UltraEasy Blood Glucose Monitoring System is a device used to measure blood glucose levels. It provides a simple and convenient way to monitor blood sugar for individuals who require regular glucose testing.
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4 protocols using onetouch ultraeasy blood glucose monitoring system
1
Fasting Blood Glucose and Insulin Assay
2
Metabolic Characterization of Fasted Mice
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Mice were fasted for 6 h (9:00 a.m. ~ 15:00 p.m.) before blood glucose measurements. Blood glucose was measured through the tail vein using the OneTouch UltraEasy Blood Glucose Monitoring System (Lifescan, Milpitas, CA, USA). Serum insulin levels were determined by a murine enzyme-linked immunosorbent assay (Shanghai Enzyme-linked Biotechnology Co., Shanghai, China). Whole blood was withdrawn from the eyeball, and plasma was separated by centrifugation at 3,000 rpm for 15 min in EDTA-K2-treated microtubes (Kangjian Medical, China). The homeostatic model assessment (HOMA) was used to quantify insulin resistance (HOMA-IR) and β cell function (%B). HOMA-IR was calculated using the following formula: HOMA-IR = (fasting glucose × fasting insulin)/22.5. HOMA %B was calculated using the following formula: HOMA-%B = (20 × fasting insulin)/(fasting glucose - 3.5) %.
3
Fasted Mice Blood Glucose and Insulin Analysis
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Mice were fasted for 6 h (9:00 a. m ∼ 15:00 p. m) before blood glucose measurements. Blood glucose was measured through tail vein using the OneTouch UltraEasy Blood Glucose Monitoring System (Lifescan, Milpitas, CA, USA). Serum insulin levels were measured by mouse enzyme-linked immunosorbent assay (Shanghai Enzyme-linked Biotechnology Co., Shanghai, China), according to manufacturer's instructions. Whole blood was withdrawn by removing eyeballs, and plasma was separated by centrifugation at 3,000 rpm for 15 min in EDTA-K2-treated microtubes (Kangjian Medical, Jiangsu, China). The homeostatic model assessment (HOMA) was the method used to quantify insulin resistance (HOMA-IR) and beta-cell function (%B). HOMA-IR was calculated using the following formula: HOMA-IR = (fasting glucose × fasting insulin)/22.5. HOMA %B was calculated using the following formula: HOMA-%B = (20 x fasting insulin)/(fasting glucose - 3.5)%.
4
Glucose Tolerance and Insulin Sensitivity
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All mice were fasted for 6 h with free access to water the morning before OGTTs and insulin tolerance tests (ITTs). Blood glucose was measured through the tail vein using the OneTouch UltraEasy Blood Glucose Monitoring System (Lifescan, Milpitas, CA, USA) 0, 15, 30, 60, and 120 min after an oral gavage of d -glucose (1.0 g/kg) at postoperative weeks 4 and 14. Blood glucose was measured 0, 15, 30, 60, and 120 min after intraperitoneal injection with human insulin (0.8 U/kg Humulin R; Novo Nordisk) at postoperative week 15. In additional experiments, fasted mice were intraperitoneally injected with sterile saline (0.9% NaCl) with or without the GLP-1R antagonist Ex-9 (50 μg; Tocris Biosciences, Bristol, UK) 120 min before the OGTT in each group at postoperative weeks 4 and 14.
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