Photographs were taken using a Nikon D5600 camera (Nikon, Japan) and Hirox digital microscope KH-8700 (Hirox Co Ltd., Japan) under white-light illumination. All image processing was done using ImageJ software37 (link).
D5600 camera
Manufactured by Nikon
Sourced in Japan
The Nikon D5600 is a digital single-lens reflex (DSLR) camera. It features a 24.2-megapixel DX-format CMOS sensor, EXPEED 4 image processor, and a 3.2-inch articulating touchscreen LCD. The camera can record full HD 1080p video at up to 60 frames per second.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using d5600 camera
1
Microscopic Image Acquisition and Analysis
2
Histological and Collagen Analysis of GTE Tissues
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The GTE tissues were processed for histological examination or for immunohistochemistry (IHC), as was previously described [23 (link),54 (link)]. In addition, tissue sections were stained with Sirius Red F3BA (Sigma-Aldrich) for collagen staining. The stained tissue sections were visualized with a BX60 microscope (Olympus, Tokyo, Japan). Images were taken at 100× with a Nikon D5600 camera (Nikon, Tokyo, Japan) at a 1/250 shutter speed and ISO 12,800 under bright field and 1/40 shutter speed and ISO 8000 under polarized light for Sirius Red F3BA staining. The experiment was run in four sample replicates (n = 4) for each group.
3
Tensile Behavior of PEEK/PEI Composites
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Five samples of each material (neat PEEK, neat PEI, PEEK/PEI@350t10, and PEEK/ PEI@350t120) were tested at ambient temperature using an Instron 8501 servohydraulic universal test machine with a loading capacity of 10 kN, following the requirements of ASTM D638 [35 ], manufacturing type V tensile test samples. For load measurement, a cross-head speed of 1 mm/min was used.
Full displacement and strain fields during the uniaxial tensile test were measured with a commercial digital image correlation code (VIC 2D, Correlated Solutions Inc., Irmo, SC, USA) [36 ] by post-processing the images extracted from a Nikon D5600 camera (magnification of 4× and resolution of 16.9 megapixel) at regular intervals. For image correlation, the speckle pattern on the surface of the samples was generated using water slide paper [37 (link)].
Full displacement and strain fields during the uniaxial tensile test were measured with a commercial digital image correlation code (VIC 2D, Correlated Solutions Inc., Irmo, SC, USA) [36 ] by post-processing the images extracted from a Nikon D5600 camera (magnification of 4× and resolution of 16.9 megapixel) at regular intervals. For image correlation, the speckle pattern on the surface of the samples was generated using water slide paper [37 (link)].
4
Neurological Deficit Assessment and Cerebral Lesion Quantification
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After treatment, the neurological deficit was evaluated by two researchers who were unaware of the groupings using a neurological deficit score as previously described (18 (link),19 (link)). Then, the extent and localization of the cerebral lesion were determined by TTC staining. Briefly, the brain was dissected in the coronal plane at the bifurcation of MCA and anterior cerebral artery, and the cerebral lesion was visualized by TTC staining. Briefly, 2-mm thick brain tissue sections were stained with 2% TTC solution at 37°C for 30 min, followed by fixation with 4% paraformaldehyde. TTC-stained sections were photographed with a D5600 camera (Nikon). The digital images were analyzed using Image-Pro Plus 6.0 software (Media Cybernetics, Inc.).
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