Rabbit anti c myc

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Rabbit anti-c-Myc is a primary antibody that recognizes the c-Myc protein, a transcription factor involved in cell growth and proliferation. This antibody can be used for various applications, including Western blotting, immunoprecipitation, and immunocytochemistry.

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C-Myc (386 citations) Anti-Myc (207 citations) Anti-c-Myc (180 citations) Rabbit anti-Myc (28 citations) Rabbit polyclonal anti-c-Myc (3 citations) Rabbit anti-TM (2 citations) Rabbit anti-c-Myc A-14 (2 citations) Rabbit polyclonal anti-c-Myc (sc-764) (2 citations) Anti-C-myc rabbit antibody (2 citations) Rabbit anti-c-Myc (N-262, (2 citations)

7 protocols using rabbit anti c myc

Western Blot Analysis of Cell Signaling

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Immunoblot analysis was performed
as previously described^{58 (link)−60 (link)} using mouse anti-β-Actin,
mouse anti-p-ERK, rabbit anti-ERK, mouse anti-p-EGFR (Y1045), mouse
anti-p-EGFR (Y1148), mouse anti-p-EGFR (Y1173), mouse anti-CCND1,
mouse anti-CDK4, mouse anti-CCNB1, rabbit anti-c-MYC, mouse anti-BCL2,
mouse anti-BCL-XL, mouse anti-BAD, rabbit anti-CYCS, mouse anti-p-ERK
(44/42), and rabbit anti-ERK antibodies were procured from Santa Cruz
Biotechnology, CA. Mouse anti-CDH1, mouse anti-CDH2, rabbit anti-OCLN,
rabbit anti-pSTAT3, and mouse anti-STAT3 antibodies were obtained
from Abcam, Cambridge, MA. Rabbit anti-p-EGFR (Y992) and rabbit anti-p-CDK2
antibodies were obtained from Cell Signaling. Cell extracts were resolved
on SDS-PAGE and immunoblotted, with the appropriate and respective
antibodies. β-Actin was used as input control for cell lysate.
The sizes of the detected protein bands are shown in kilodaltons on
the left side.

Sebastian A., Pandey V., Mohan C.D., Chia Y.T., Rangappa S., Mathai J., Baburajeev C.P., Paricharak S., Mervin L.H., Bulusu K.C., Fuchs J.E., Bender A., Yamada S., B.a.s.a.p.p.a., Lobie P.E, & Rangappa K.S. (2016). Novel Adamantanyl-Based Thiadiazolyl Pyrazoles Targeting EGFR in Triple-Negative Breast Cancer. ACS Omega, 1(6), 1412-1424.

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Wnt Signaling Pathway Analysis

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Protein extraction, SDS-PAGE separation, and visualization were performed as described [73 (link)], except for the detection step (Immobilon Forte, Millipore WBLUF0500) and band intensity quantification (ImageJ software). Western blot analysis of media was performed similarly, without a lysis step (media were directly diluted with sample buffer X5 and denaturized by heating).
Antibodies: mouse anti-Actin (MP biomedical 69100, 1:10,000), mouse anti-β-catenin (BD 610154, 1:2000), rabbit anti-c-Myc (Santa Cruz 788, 1:500), rabbit anti-Frizzled (Santa Cruz Biotechnology 9169, 1:500), mouse anti-GSK3 (Santa Cruz Biotechnology 7291, 1:500), rabbit anti-LEF1 (Abcam 137872, 1:1000), mouse anti-LRP5 (Abcam 129357, 1:500), rabbit anti-LRP6 (Abcam 134146, 1:1000), mouse anti-TCF4 (Millipore 05–511, 1:1000), rabbit anti-Wnt-3a (Cell Signaling 2721, 1:3000), Goat anti-mouse HRP (Jackson ImmunoResearch 115–035-003, 1:10000), Goat anti-rabbit HRP (Jackson ImmunoResearch 111–035-144, 1:10000).

Zelikson N., Ben S., Caspi M., Tarabe R., Shaleve Y., Pri-Paz Basson Y., Tayer-Shifman O., Goldberg E., Kivity S, & Rosin-Arbesfeld R. (2024). Wnt signaling regulates chemokine production and cell migration of circulating human monocytes. Cell Communication and Signaling : CCS, 22, 229.

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Glomerular Mesangial Cell Response to Compound 1

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Rat glomerular mesangial cells (RMC, HBZY-1, Life-Science Academy of Wuhan University, Wuhan, China) were cultured and maintained in DMEM (Invitrogen, Carlsbad, CA), PH7.4, supplemented with 10% fetal bovine serum (FBS, Invitrogen, Carlsbad, CA), 2 mM glutamine, 100 U/ml penicillin, 100 µg/ml streptomycin at 37 °C. To examine the effect of compound 1, RMC cells were pre-treated with indicated concentration of compound 1 at 37 °C for 1 h, and then exposed to either 5.6 mM (normal glucose, NG) or 25 mM (high glucose, HG) D-glucose for 24 h or 48 h. Whole cell proteins were extracted using a cell lysis buffer kit (Cell signaling, MA, USA) and quantified by the Bradford assay (Bio-Rad, Hercules, CA). The equivalent amount of proteins were resolved with SDS-PAGE and transferred to nitrocellulose membranes. The membranes were blocked and then incubating with a rabbit anti-fibronectin pAb (Sigma, MO, USA), rabbit anti-collagen I (Abcam, MA, USA), rabbit anti-p47phox, rabbit anti-Nox4, rabbit anti-PCNA, rabbit anti-c-myc (all from Santa Cruz, Texas, USA) and mouse anti-α-tubulin (Sigma) at 4 °C overnight. After washing, the membranes were incubated with HRP-conjugated anti-rabbit or anti-mouse IgG secondary antibodies and detected by using ECL detection system.

Lin X., Wu Q., Yu Y., Liang Z., Liu Y., Zhou L., Tang L, & Zhou X. (2017). Penicilliumin B, a novel sesquiterpene methylcyclopentenedione from a deep sea-derived Penicillium strain with renoprotective activities. Scientific Reports, 7, 10757.

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Drosophila Tissue Immunofluorescence Staining

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Drosophila S2 cells grown on cover slips, dissected ring glands and imaginal discs were processed as described [65] (link) and stained overnight at 4°C (tissues) or 2 h at room temperature (S2 cells) with the following antibodies: rat anti-Ecd^Nterm (1∶500, this study), guinea pig anti-Spok (1∶1000) and rabbit anti-Phm (1∶300) [33] (link), rabbit anti-cleaved Caspase-3 (1∶500, ASP175, Cell Signaling, #9661), rabbit anti-pH3 (1∶100, Cell Signaling, #9701), mouse anti-Flag M2 (1∶500, Sigma Aldrich), rabbit anti-c-Myc (1∶500, sc-789, Santa Cruz), and mouse anti-Lamin (1∶500, ADL67.10), mouse anti-EcR (1∶200, DDA2.7), and mouse anti-Fasciclin III (1∶300); the latter three from the Developmental Studies Hybridoma Bank (DSHB, Iowa). After washing, samples were incubated with corresponding secondary antibodies coupled to Cy3 or Cy5 (Jackson ImmunoResearch), counterstained with DAPI (0.5 µg/ml, Invitrogen) to visualize nuclei, and mounted in Dabco:Mowiol (Sigma-Aldrich).

Claudius A.K., Romani P., Lamkemeyer T., Jindra M, & Uhlirova M. (2014). Unexpected Role of the Steroid-Deficiency Protein Ecdysoneless in Pre-mRNA Splicing. PLoS Genetics, 10(4), e1004287.

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Investigating Apoptotic Signaling Pathways

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Goat anti-CYP2D6, rabbit anti-c-Myc, anti-cyclin D1, anti-Rb, anti-p-Rb, anti-PARP-1, anti-Bak, anti-Bax, anti-caspase-3, anti-caspase-9, anti-GRP 94, anti-GRP 78, mouse anti-Bcl-2 and anti-Bcl-xL antibodies were purchased from Santa Cruz (Santa Cruz, CA). Mouse anti-caspase-7, anti-mouse, anti-rabbit, and anti-goat horseradish peroxidase (HRP)-linked antibodies were purchased from Cell Signaling (Danvers, MA). Mouse anti-β-actin antibody, risperidone, paliperidone, tamoxifen, 4-hydroxytamoxifen, endoxifen, fluoxetine, crystal violet, 3-(4,5-cimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), sulforhodamine B (SRB), dimethyl sulfoxide (DMSO), propidium iodide (PI) were purchased from Sigma-Aldrich (St. Louis, MO). RNase A was purchased from Amresco (Solon, OH).

Yeh W.L., Lin H.Y., Wu H.M, & Chen D.R. (2014). Combination Treatment of Tamoxifen with Risperidone in Breast Cancer. PLoS ONE, 9(6), e98805.

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Metabolic Enzyme Profiling in Embryos

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The following antibodies and drugs were used in this study. Fluorescein Phalloidin (Thermo Fisher #F432, at 1:2000 dilution), rabbit anti-PKM2 (CST #4053), goat anti-PFK1 (Santa Cruz #sc-31712), mouse anti-GAPDH (Santa Cruz #sc-32233), rabbit anti-GLS (Abcam #ab93434), rabbit anti-GLUD1 (Abcam #ab168352), and rabbit anti-c-Myc (Santa Cruz #sc-764). All the antibodies are used at 1:100 dilution for IF.
The chemical reagents used in this study were: YZ9 (Cayman Chemical #15352, 1μM), KJ-Pyr-9 (Cayman Chemical #19116, 20nM), CB-839 (Cayman Chemical #22038, 100nM), O-(Carboxymethyl)hydroxylamine hemihydrochloride (AOA) (Sigma #C13408, 0.5mM), L-Methionine sulfoximine (MSO) (Sigma #M5379, 1mM), Etomoxir (Sigma #E1905, 5μM), UK-5099 (Sigma # PZ0160, 500nM). The inhibitors were used at the time indicated in the text to treat groups of 30 embryos, with each treatment performed on several independent cohorts of embryos. To avoid absorption of inhibitors into the oil-overlay, hydrophobic inhibitors were provided to embryos that were cultured under oil-free conditions.

Sharpley M.S., Chi F., Hoeve J.T, & Banerjee U. (2021). Metabolic Plasticity drives Development during Mammalian Embryogenesis. Developmental cell, 56(16), 2329-2347.e6.

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Neuronal Protein Extraction and Quantification

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Cultures were transduced at DIV2 with low-titer virus. Lysates were then harvested between DIV10-12 in Sodium Tris EDTA NP40 (STEN) buffer containing 50 mM Tris HCl pH 7.6, 150 mM NaCl, 2 mM EDTA, 0.2% NP-40, 1% Triton X-100 and 2% protease inhibitors (complete protease inhibitor cocktail, Roche) in H 2 O. Media was removed from each well and replaced with 60 ml of chilled STEN buffer. After 30 min incubation on a shaker at 4 C, cells were scraped, collected, and stored in tubes on ice for 10 min. Samples were then centrifuged for 10 min at 4 C at 14,800 rpm. Supernatant was collected. Protein estimation was performed using the Bio-Rad protein assay kit (500-0006).
Antibodies used were the following: sheep anti-LRRTM1 (R&D Systems), goat anti-FLRT2 (R&D Systems), goat anti-Slitrk1 (R&D Systems), mouse anti-PSD-95 (ThermoScientific), rabbit anti-c-Myc (Santa Cruz Biotechnology), rabbit anti-HA (Sigma), rabbit anti-bIII-tubulin (Abcam), mouse anti-Synaptophysin (Sigma), mouse anti-b-actin (Sigma). Western blot images were quantified in Image Studio Lite (LI-COR Biosciences). Identical ROIs were used for each band on any given blot. Signal intensities were quantified and background was subtracted. For display purposes only, original western blot images were adjusted for brightness and contrast.

Schroeder A., Vanderlinden J., Vints K., Ribeiro L.F., Vennekens K.M., Gounko N.V., Wierda K.D, & de Wit J. (2018). A Modular Organization of LRR Protein-Mediated Synaptic Adhesion Defines Synapse Identity. Neuron, 99(2).

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