Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore) was used for RIP assay in accordance with the instructions. The processed cells were conjugated with anti-SUZ12 antibody (#3737, 1/100 dilution, Cell signaling technology) and control IgG antibody (Millipore) on magnetic beads. After digestion, the precipitated RNAs were extracted for RT-qPCR analysis.
Anti suz12 antibody
Manufactured by Cell Signaling Technology
Sourced in United States
The Anti-SUZ12 antibody is a laboratory reagent used for the detection and analysis of the SUZ12 protein in various biological samples. SUZ12 is a core component of the Polycomb Repressive Complex 2 (PRC2), which is involved in the epigenetic regulation of gene expression. The antibody can be utilized in techniques such as Western blotting, immunoprecipitation, and immunohistochemistry to study the expression and localization of the SUZ12 protein.
Automatically generated - may contain errors
Lab products found in correlation
Thermo fisher rip kit, by Thermo Fisher Scientific (2 mentions)
Human anti ezh2 antibody, by Cell Signaling Technology (2 mentions)
Magna rip rna binding protein immunoprecipitation kit, by Merck Group (1 mentions)
Control igg antibody, by Merck Group (1 mentions)
Nc normal mouse igg, by Merck Group (1 mentions)
3 protocols using anti suz12 antibody
1
SUZ12 RNA-Binding Protein Immunoprecipitation
2
RIP Assay for EZH2 and SUZ12
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
RIP was performed using a Thermo Fisher RIP Kit (Thermo Fisher, Waltham MA, USA) based on the manufacturer’s protocol. Cells at 80%–90% confluency were scraped off and lysed in complete RIP lysis buffer. One hundred microliters of whole-cell extract was then incubated with RIP buffer containing magnetic beads conjugated with human anti-EZH2 antibody (Cell Signaling Technology), anti-SUZ12 antibody (Cell Signaling Technology), and NC normal mouse IgG (Millipore).
3
RNA Immunoprecipitation of Polycomb Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
RNA immunoprecipitation was performed using thermo fisher RIP kit (Thermo, USA) based on the manufacturer’s protocol. Cells at 80–90% confluency were scraped off and lysed in complete RIP lysis buffer. Then, 100 μl of whole cell extract was incubated with RIP buffer containing magnetic beads conjugated with human anti-EZH2 antibody (Cell Signaling, USA), anti-SUZ12 antibody (Cell Signaling, USA), anti-EED antibody (Cell Signaling, USA) negative control normal mouse IgG (Millipore).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!